Sage Journals: Discover world-class research

Abstract

This study investigates cross-talk of the related bioactivity mediators in silica-induced pulmonary inflammatory and fibrosis on rats, which contributes to the preventive and therapeutic effect of soluble TNF-α receptor. Wistar rats received saline or 50 mg of quartz by intratracheal instillation. Rats in drug-treated groups were given soluble tumor necrosis factor-α (TNF-α) receptor (500 μg) by hypodermic injection on days 1, 5 and 8 after operation. At 7 days or 14 days after instillation, rats were killed to observe the degree of injury and expression of the related bioactivity mediators including nuclear factor KB (NF-KB), nitric oxide, interleukin-1β, interleukin-10, transforming growth factor beta 1 (TGF-β1), TNF-α, interferon-Y (IFN-Y) and granulocyte macrophage colony-stimulating factor (GM-CSF). The area percentages of type I and III collagens in intervention group were lower than those in silica group. The expression of NF-κB, TGF-β1, and COL I were lower in intervention group than in silica group(p < 0.05) and GM-CSF was significantly higher (p < 0.05) at 7 days after instillation, however, NF-κB, TGF-β1, and COL I were identically lower in intervention group than in silica group, and TNF-α, IFN-γ, and GM-CSF were higher at 14 days after instillation. It may be concluded that soluble TNF-α receptor upregulating or downregulating the expression of the related bioactivity mediators results in decreasing lung injury induced by silica.

Keywords

Silicosis soluble TNF-α receptor bioactivity mediator TGF-β NF-κB IFN-γ

Get full access to this article

View all access options for this article.

References

Berclaz PY , Carey B. , Fillipi MD , Wernke-Dollries K. , Geraci N. , Cush S. , et al. (2007) GM-CSF Regulates a PU.1-Dependent Transcriptional Program Determining the Pulmonary Response to LPS. American Journal of Respiratory Cell and Molecular Biology 36(1): 114-121.

Broide DH , Stachnick G. , Castaneda D. , Nayar J. , and Sriramarao P. ( 2001) Inhibition of eosinophilic inflammation in allergen-challenged TNF receptor p55/p75-and TNF receptor p55-deficient mice. American Journal of Respiratory Cell and Molecular Biology 24: 304-311.

Cheong R. , Hoffmann A. , and Levchenko A. ( 2008) Understanding NF-κB signaling via mathematical modeling . Molecular and System Biology 4: 192.

Crisby M. , Nordin-Fredriksson G. , Shah PK , Yano J. , Zhu J. , and Nilsson J. ( 2001) Pravastatin treatment increases collagen content and dreasea lipid content, inflammation, metalloproteinases, and cell death in human carotid plaques. Circulation 103: 926-933.

Di Giuseppe M. , Gambelli F. , Hoyle GW , Lungarella G. , Studer SM , Richards T. , et al. (2009) Systemic Inhibition of NF-κB Activation Protects from Silicosis. PLoS ONE 4(5): e5689.

Gabbiani G. ( 2003) The myofibroblast in wound healing and fibrocontractive diseases. Journal of Pathology 200: 500-503.

Giannopoulou M. , Iszkula SC , Dai C. , Tan X. , Yang J. , Michalopoulos GK , et al. (2006) Distinctive role of Stat3 and Erk-1/2 activation in mediating interferon-γ inhibition of TGF-β1 action. American Journal of Physiology and Renal Physiology 290(5): 1234-40.

Greten FR and Karin M. ( 2004) The IKK/NF-κB activation pathway-A target for prevention and treatment of cancer. Cancer Letter 206:193-199.

Hubbard AK , Timblin CR , Shukla A. , Rincón M. , and Mossman BT ( 2002) Activation of NF-κB dependent gene expression by silica in lungs of luciferase reporter mice. American Journal of Physiology and Lung Cell Molecular Physiolology 282:968-975.

10.

Jiang D. , Liang J. , Hodge J. , Lu B. , Zhu Z. , Yu S. , et al.(2004) Regulation of pulmonary fibrosis by chemokine receptor CXCR3. Journal of Clinical Investment 114(2): 291-299.

11.

Kolb M. , Margetts PJ , Anthony DC , Pitossi F. , and Gauldie J. ( 2001) Transient expression of IL-1β induces acute lung injury and chronic repair leading to pulmonary fibrosis. Journal of Clinical Investment 107(12): 1529-1536.

12.

Moore BB , Coffey MJ , Christensen P. , Sitterding S. , Ngan R. , Wilke CA , et al. (2000) GM-CSF Regulates bleomycin-induced pulmonary fibrosis via a prostaglandin-dependent mechanism. Journal of Immunology 165(7): 4032-4039.

13.

Nam HS , Lee SY , Kim SJ , Kim JS , Kwon SS , Kim YK , et al. (2009) The Soluble Tumor Necrosis Factor-Alpha Receptor Suppresses Airway Inflammation in a Murine Model of Acute Asthma. Yonsei Medecine of Journal 50(4): 569-575.

14.

Paul J. , Marc B. Bailie, Richard E. Goodman , Aidan D. O’Brien, Galen B. Toews , and Robert Paine III (2000) Role of diminished epithelial GM-CSF in thepathogenesis of bleomycin-induced pulmonary fibrosis. American Journal of Physiology and Lung Cell Molecular Physiology 279: 487-495.

15.

Robertson M. , Liversidge J. , Forrester JV , and Dick AD ( 2003) Neutralizing tumor necrosis factor activity suppresses activation of infiltrating macrophages in experimental autoimmune uveoretinitis . Investigative Ophthalmology & Visual Science. 2003; 44(7):3034-3041.

16.

Sakurai H. , Miyoshi H. , Toriumi W. , and Sugita T. ( 1999) Functional interactions of TGF-β-activated kinase 1 with IκB kinases to stimulate NF-κB activation. Journal of Biological Chemistry 274: 10641-10648.

17.

Sakurai H. , Nishi A. , Sato N. , Mizukami J. , Miyoshi H. , and Sugita T. ( 2002) TAK1-TAB1 fusion protein: a novel constitutively active mitogen-activated protein kinase kinase kinase that stimulates AP-1 and NF-κB signaling pathways. Biochemical and Biophysical Research Communications 297(5):1277-1281.

18.

Srirupa Mukhopadhyay , John R Hoidal , and Tapan K Mukherjee (2006) Role of TNF-α in pulmonary pathophysiology. Respiratory Research 7(1): 125.

19.

Takaesu G. , Surabhi RM , Park KJ , Ninomiya-Tsuji J. , Matsumoto K. , and Gaynor RB ( 2003) TAK1 is critical for IκB kinase-mediated activation of the NF-κB pathway. Journal of Molecular Biology 326(1): 105-115.

20.

Ye Q. , Chen B. , Tong Z. , Nakamura S. , Sarria R. , Costabel U. , et al. (2006) Thalidomide reduces IL-18, IL-8 and TNF-alpha release from alveolar macrophages in interstitial lung disease. European Respiratory Journal 28(4): 824-831.

21.

Xing Z. , Gauldie J. , Tremblay GM , Hewlett BR , and Addison C. ( 1997) Intradermal transgenic expression of granulocyte-macrophage colony-stimulating factor induces neutrophilia, epidermal hyperplasia, Langerhans’ cell/macrophage accumulation, and dermal fibrosis. Laboratory Investigation 77(6): 615-622.

Cross-talk of the related bioactivity mediators in serum after injection of soluble TNF-α receptor on silicosis model of rats

Abstract

Keywords

Get full access to this article

References