Elaidic acid enhanced the simultaneous neurotoxicity attributable to the cerebral pathological lesion resulted from oxidative damages induced by acrylamide and benzo(a)pyrene

Abstract

Acrylamide (ACR), benzopyrene [B(a)P] and trans-fatty acids (TFA) could be found to co-exist in many foods processed by high temperature. Our study investigated the effects of elaidic acid (ELA), a predominant TFA, on neuropathology induced by simultaneous exposure of ACR and B(a)P to mice. Results showed ELA enhanced the decrease of weight gains induced by simultaneous exposure of ACR and B(a)P (AB). Moreover, ELA enhanced ACR-induced increase of gait abnormality, B(a)P-induced damage to learning and memory, and AB-induced both of the damage above. Meanwhile, ELA enhanced B(a)Pinduced axonal degeneration in hippocamp, ACR- and AB-induced up-regulating of abnormal cerebellar Purkinje cells. ELA enhanced ACR-induced up-regulating of MDA in cerebrum and 8-OHdG in cerebrum and cerebellum; ELA enhanced B(a)P-induced up-regulating of MDA in cerebrum, PCO in cerebellum and 8-OHdG in cerebrum and cerebellum. Meanwhile, the enhancing role of ELA, on ACR-induced reduction of SOD activity in cerebrum and cerebellum, on B(a)P-induced reduction of GPx activity in cerebrum were found. Results suggested that ELA play a enhancing role on ACR-induced and B(a)P-induced oxidative damage, which attributable to the cerebral pathological lesion, and subsequent effect on gait abnormality and deficit on learning and memory in mice.