Abstract
The aim of this study was to investigate the effects of methanol, acetone, n-hexane and ether extracts obtained from Pseudovernia furfuracea on genotoxicity and total antioxidant capacity (TAC) in cultured human blood cells intoxicated with aflatoxin B 1 (AFB1). Sister chromatid exchange (SCE) and micronucleus (MN) tests were used for genotoxic influences estimation. In both the test systems, it was observed that P. furfuracea extracts suppressed the mutagenic effects of AFB1 due to the type of extracts added to the cultures. Furthermore, a significant reduction in plasma TAC was observed after AFB 1 treatment. Interestingly, the methanol and acetone extracts of the lichen recovered AFB1-induced TAC inhibition. The order of extracts of anti-genotoxicity efficacy against AFB1 was methanol, acetone, ether and n-hexane, respectively. In conclusion, P. furfuracea has been shown to modulate the adverse effects of AFB1 in human blood cells for the first time.
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