In this study, gasoline vapors-induced hematotoxicity, growth-depression and weight-loss reversal effect of vitamins A (retinol) and E (α-tocopherol) was assessed in female Wistar albino rats. The rats were exposed to gasoline vapors (17.8 2.6 cm 3/h/m3/day), 6 hours/day, 6 days/week, for 20 weeks. Vitamins A and E at prophylactic dosage (400 and 200 IU/kg/day, respectively) were orally administered to the rats, separately, in the last 2 weeks of exposure. The levels of hemoglobin (Hb), hematocrit or packed cell volume (PCV), red blood cells (RBC), growth rate and weight gain in the rats exposed to the vapors were significantly lower (p < 0.05) compared, respectively, to the levels obtained for control rats. On the other hand, the levels of white blood cells (WBCs) in the test rats were significantly higher (p < 0.05) compared, respectively, with the level obtained for female control rats. These observations indicated that exposure to gasoline vapors may cause hematotoxicity, growth depression and weight loss in female rats. However, administration of vitamins A and E was observed to produce a significant recovery (p < 0.05) in hematotoxicity, growth depression and weight loss observed to be associated with exposure to gasoline vapors, although the rats administered with vitamin E were noted to respond more favorably than those administered with vitamin A. This suggests that although retinol and α-tocopherol may be used to reverse or prevent hematotoxicity, growth depression and weight loss in subjects exposed to gasoline vapors, the reversal potency of α-tocopherol is higher than that of retinol.
Alexander RR, Griffiths JM ( 1993a) Hematocrit. In: Basic Biochemical Methods . 2nd ed. New York: John Willey & Sons, pp. 186-187.
2.
Alexander RR, Griffiths JM ( 1993b) Hemoglobin determination by the cyanmethhemoglobin method . In: Basic Biochemical methods. 2nd ed. New York: John Willey & Sons. pp. 188-189.
3.
Amarnath V., Anothony DC, Valentine WM, and Graham DG ( 1991) The molecular mechanism of the carbon disulphide mediated cross-linking of proteins. Chemical Research in Toxicology4: 148-150.
4.
Andersson TLG, Matz J., Gerns GAA, and Änggard EE ( 1994) Vitamin E reverses cholesterol-induced endothelial dysfunction in the rabbit coronary circulation. Atherosclerosis111: 39-45.
5.
Carballo M., Nigro ML, Fraga I., and Gadano A. ( 1994) Ethylene oxide: cytogenetic and biochemical studies in persons occupationally exposed. Environmental and Molecular Mutagenesis23: 7-12.
6.
Chatterjea MN, Shinde R. ( 2002) Growth factors. In: Textbook of Medical Biochemistry (5th ed). New Delhi : Jaypee Brothers Medical Publishers Ltd , pp. 714-716.
7.
Cotran RS, Kumar V., and Collins T. ( 1999) Cellular pathology I &II. In: Robbins pathologic basis of disease. 6th ed. Philadelphia: WB Sanders Co, pp. 1-49.
8.
Dacie JV, Lewis SM ( 1975) Practical haematology. 4th ed. London: Churchill Livingstone Publishers , pp. 214-244.
9.
d’Azevedo PA, Tannhauser AL, Tannhauser SL, et al. (1996) Hematological alterations in rats from xylene and benzene. Veterinary and Human Toxicology38: 340-344.
10.
EHC20 (1982) Selected petroleum products. In: Environmental Health Criteria 20, United Nations Environment Programme. Geneva: The International Organisation and World Health Organisation, 5-8.
11.
Heitzer T., Herttuala SY, Wild E., Luoma J., and Drexler H. ( 1999) Effect of vitamin E on endothelial vasodilator function in patients with hypercholesterolemia, chronic smoking or both. Journal of American College of Cardiology33: 499-505.
12.
Jialal I., Grundy SM ( 1993) Effect of combined supplementation with alpha-tocopherol, ascorbate, and beta carotene on low-density lipoprotein oxidation. Circulation88: 2780-2786.
13.
Knert P., Jarvinen R., Teppo L., Aroma A., and Seppanen R. ( 1999) Role of various carotenoids in lung cancer prevention . Journal of National Cancer Institute91: 182-184.
14.
Kushi LH, Folsom AR, Prineas RJ, Mink PJ, Wu Y., and Bostick R. ( 1996) Dietary antioxidant vitamins and death from coronary heart disease in postmenopausal women. New England Journal of Medicine334: 1156-1162.
15.
Lotan R. ( 1999) Lung cancer promotion by β-carotene and tobacco smoke: relationship to suppression of retinoic acid receptor-β and increased activator protein-1. Journal of National Cancer Institute91: 7-9.
16.
Maellaro E., Deal-Beelo BD, Syherini L., Pompella A., Casin A., and Comporti M. ( 1994) Protection by ascorbic acid against oxidative injury of isolated hepatocytes. Xenobiotica24: 281-289.
17.
Machlin LJ, Bendich A. ( 1999) Free radical tissue damage: Protective role of antioxidant nutrients. European Journal of Clinical Nutrition . 53: 216-225.
18.
Marcus R., Coulston AM ( 1996) Fat-soluble vitamins: vitamins A, E and K. In: JG Hardman, LE Limibird (eds) Goodman and Gulman: The Pharmacological basis of Therapeutics. New York: McGraw Hill Co, pp. 1573-1590.
19.
Massague J. ( 1998) TGF-β signal transduction. Annual Review of Biochemistry67: 733.
20.
Moser GJ, Wong BA, Wolf DC, Moss OR, and Goldsworthy TL ( 1996) Comparative short-term effects of methyl tertiary butyl ether and unloaded gasoline vapour in female B6C3F1 mice. Fundamental and Applied Toxicology31: 173-183.
21.
Murray RK ( 2000a) Polypeptide growth factors are mitogenic. In: Murray RK, Granner PA, Mayes and Rodwell VW (eds) Harper’s Biochemistry. 25th ed. New York: McGraw-Hill, pp. 796-799.
22.
Murray RK ( 2000b) Red & white blood cells. In: Granner RK, Mayes PA and Rodwell VW (eds) Harpers’s Biochemistry . New York: McGraw-Hill, pp. 780-786.
23.
Peshlow L., Hesse S. ( 1991) The milk thistle (Silyhum marianum) and silymarin as hepatic therapeutic agents. Zeitschrift Fur Phytotherapy12: 162-174.
24.
Rabble GK, Wong O. ( 1996) Leukaemia mortality by cell type in petroleum workers with potential exposure to benzene. Environmental Health Perspectives104: 1381-1392.
25.
Reaven PD, Khouw A., Beltz WF, Parthasarathy S., and Witztum JL ( 1993) Effect of dietary antioxidant combinations in humans . Arteriosclerosis and Thrombosis13: 590-600.
26.
Rothman N., Li GL, Dosemeci M., Bechtold WE, Marti GE, Wang YZ, et al. (1996) Hematotoxicity among Chinese workers heavily exposed to benzene. American Journal of Industrial Medicine29: 236-246.
27.
Sheweita SA, Abd El-Gabar M., and Bastawy M. ( 2001) Carbontetrachloride-induced changes in the activity of Phase II drug-metabolozing enzyme in the liver of male rats: role of antioxidants . Toxicology165: 217-224.
28.
Sporn MN, Roberts AB ( 1983) Role of retinoids in diferentiation and carcinogenesis . Cancer Research43: 3034.
29.
Standeven AM, Goldsworthy TL ( 1994) Identification of hepatic mitogenic and cytochrome P-450-inducing fractions of unleaded gasoline in B6C3F1 mice. Journal of Toxicology and Environmental Health43: 213-224.
30.
Synder R., Hedhi CC ( 1996) An overview of benzene metabolism (review). Environmental Health Perspectives104: 1165-1171.
31.
Tilbury L., Bulterworth BE, Moss O., and GoldworthyTL (1993) Hepatocyte cell proliferation in mice after inhalation exposure to unleaded gasoline vapour. Journal of Toxicology and Environmental Health38: 293-307.
32.
Uboh FE, Ebong PE, Eka OU, Eyong EU, and Akpanabiatu MI ( 2005) Effect of inhalation exposure to kerosene and petrol vapors on some anaemia-diagnostic indices in rats. Gobal Journal of Environmental Science4: 60-65.
33.
Uboh FE, Akpanabiatu MI, Atangwho IJ, Ebong PE, and Umoh IB ( 2008) Effect of vitamin A on weight-loss and hematotoxicity associated with gasoline vapors exposure in rats. International Journal of Pharmacology4: 40-45.
34.
Valentine WM, Amarnath V., Graham DG, and Anthony DC ( 1993) Covalent cross-linking of erythrocytes spectrin by carbon disulphide in vivo. Toxicology and Applied Pharmacology121: 71-77.
35.
Verlangieri AJ, Bush MJ ( 1992) Effects of d-alpha-tocopherol supplementation on experimentally induced primate atherosclerosis. Journal of American College Nutrition11: 131-138.
36.
Zahlsen K., Nilsen AM, Eide I., and Nilsen OG ( 1993) Inhalation kinetics of carbon-8 to Carbon-10, L-alkanes and iso-alkanes in the rats after repeated exposures. Pharamacology and Toxicology73: 163-168.
