This study was designed to characterize and compare the effects of jet propellant-8 (JP-8) fuel and synthetic-8 (S-8) on cell viability and nitric oxide synthesis in cultured alveolar type II epithelial cells of rats. Exposure times varied from 0.25, 0.5, 1, and 6 hours at the following concentrations of jet fuel: 0.0, 0.1, 0.4, and 2.0 µg/mL. Data indicate that JP-8 presents a gradual decline in cell viability and steady elevation in nitric oxide release as exposure concentrations increase. At a 2.0 µg/mL concentration of JP-8, nearly all of the cells are not viable. Moreover, S-8 exposure to rat type II lung cells demonstrated an abrupt fall in percentage cell viability and increases in nitric oxide measurement, particularly after the 2.0 µg/mL was reached at 1 and 6 hours. At 0.0, 0.2, and 0.4 µg/mL concentrations of S-8, percentage viability was sustained at steady concentrations. The results suggest different epithelial toxicity and mechanistic effects of S-8 and JP-8, providing further insight concerning the impairment imposed at specific levels of lung function and pathology induced by the different fuels.
Anand SS, Campbell JL, and Fisher JW ( 2007) In vitro rat hepatic metabolism of nalkanes: nonane, decane, and tetradecane. International Journal of Toxicology26: 325-329.
2.
Chao YC, Kupper LL, Serdar B., Egeghy PP, Rappaport SM, and Nylander-French LA (2006) Dermal exposure to jet fuel JP-8 significantly contributes to the production of urinary naphthols in fuel-cell maintenance workers. Environmental Health Perspective114: 182-185.
3.
Cooper JR, Mattie DR ( 1996) Developmental toxicity of JP-8 jet fuel in the rat. Journal of Applied Toxicology16, 197-200.
4.
Dossing M., Loft S., and Schroeder E. ( 1985) Jet fuel and liver function. Scandinavian Journal of Work Environment, and Health11: 433-437.
5.
Espinoza LA, Tenzin F., Cecchi AO, Chen Z., Witten ML, and Smulson ME ( 2006) Expression of JP-8-induced inflammatory genes in AEII cells is mediated by NFkappaB and PARP-1. American Journal of Respiratory Cell Molecular Biology35: 479-487.
6.
Harris DT, Sakiestewa D., Robledo RF, and Witten M. ( 1997) Immunotoxicological effects of JP-8 jet fuel exposure . Toxicology and Industrial Health13: 43-55.
7.
Hays AM, Parliman G., Pfaff JK, Lantz RC, Tinajero J., Tollinger B., et al. (1995) Changes in lung permeability correlate with lung histology in a chronic exposure model. Toxicology and Industrial Health11: 325-336.
8.
Inman AO, Monteiro-Riviere JE ( 2008) Inhibition of jet fuel aliphatic hydrocarbon induced toxicity in human epidermal keratinocytes. Journal of Applied Toxicology28: 543-553.
9.
Keil DE, Warren DA, Jenny MJ, EuDaly JG, Smythe J., and Peden-Adams MM ( 2003) Immunological function in rats exposed to JP-8 jet fuel in utero. Toxicological Sciences76: 347-356.
10.
Keil D., Dudley A., EuDaly J., Dempsey J., Butterworth L., Gilkeson G., et al. (2004) Immunological and hematological effects observed in B6C3F1 rats exposed to JP-8 jet fuel for 14 days. Journal of Toxicology, Environment, and Health A67: 1109-1129.
11.
Knave B., Mindus P., and Struwe G. ( 1979) Neurasthenic symptoms in workers occupationally exposed to jet fuel. Acta Psychiatrica Scandinavica60: 39-49.
12.
Koschier FJ ( 1999) Toxicity of middle distillates from dermal exposure. Drug Chemical Toxicology22: 155-164. Mason RJ (2006) Biology of alveolar type II cells. Respirology ( Carlton, Vic.)11: 12-15.
13.
McDougal JN, Rogers JV ( 2004) Local and systemic toxicity of JP-8 from cutaneous exposures . Toxicology Letters149: 301-308.
14.
Preuss R., Angerer J., and Drexler H. ( 2003) Naphthalene- an environmental and occupational toxicant . International Archives of Occupational and Environmental Health76: 556-576.
15.
Robledo RF, Witten ML ( 1999) NK1-receptor activation prevents hydrocarbon induced lung injury in rats. American Journal of Physiology276: L229-L238.
16.
Robledo RF, Barber DS, and Witten ML ( 1999) Modulation of bronchial epithelial cell barrier function by in vitro jet propulsion fuel 8 exposure. Toxicological Sciences51: 119-125.
17.
Smith LB, Bhattacharya A., Lemasters G., Succop P., Puhala E., Medvedovic M., et al. (1997) Effect of chronic low-level exposure to jet fuel on postural balance of US Air Force personnel. Journal of Occupational and Environmental Medicine39: 623-632.
18.
Struwe G., Knave B., and Mindus P. ( 1983) Neuropsychiatric symptoms in workers occupationally exposed to jet fuel-a combined epidemiological and casuistic study. Acta Psychiatrica Scandinavica Supplementum303: 55-67.
19.
Sun NN, Wong SS, Nardi C., Ostroff D., and Witten M. ( 2007) In vitro pro-inflammatory regulatory role of Substance P in alveolar macrophages and type II pneumocytes after JP-8 exposure. Journal of Immunotoxicology4:1, 61-67.
20.
Ullrich SE, Lyons HJ ( 2000) Mechanisms involved in the immunotoxicity induced by dermal application of JP-8 jet fuel. Toxicological Sciences58: 290-298.
21.
US Air Force (1996) History of Aviations Fuel Development in the US Air Force Research Laboratory, Propulsion Directorate, FuelsBranch. US Air Force.
22.
Wang S., Young RS, Witten ML ( 2001) Age-related differences in pulmonary inflammatory responses to JP-8 jet fuel aerosol inhalation. Toxicology and Industrial Health17: 23-29.
23.
Witzmann FA, Bauer MD, Fieno AM, Grant RA, Keough TW, Kornguth SE, et al. (1999) Proteomic analysis of simulated occupational jet fuel exposure in the lung. Electrophoresis20: 3659-3669.
24.
Wong SS, Hyde J., Sun NN, Lantz RC, Witten ML ( 2004) Inflammatory responses in rats sequentially exposed to JP-8 jet fuel and influenza virus. Toxicology197: 139-147.
25.
Wong SS, Vargas J., Thomas A., Fastje C., McLaughlin M., Camponovo R., et al. (2008) In vivo comparison of epithelial responses for S-8 versus JP-8 jet fuels below permissible exposure limit. Toxicology254: 106-111.
