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Abstract

Application of different types of organic wastes may have a marked effect on soil microbial biomass and its activity. The objective of this study was to quantify the amount of microbial biomass in a loamy-clayey soil, amended with different types of organic waste residues (composts of municipal solid waste of different ages, sewage sludge and farmyard manure) and incubated for 8 weeks at 25°C and two-thirds of field capacity, using the fumigation–extraction method. Both microbial biomass-C and -N (B _C and B _N, respectively) appeared to be dependent on the type of organic waste residues, on their degree of stability, and on their chemical characteristics. In general, organic wastes increased the microbial biomass-C content in the soil and the microbial B _C was positively correlated with the organic C content, the C/N, neutral detergent fibre/N (NDF/N) and acid detergent fibre/N (ADF/N) ratios. The microbial biomass content decreased according to the period of incubation, especially when the compost used was immature. The microbial biomass-N was positively correlated with the total N and percentage of hemicellulose. The microbial biomass-C was linearly related with the microbial biomass-N and the ratio B _C/B _N was exponentially related with the B _C.

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References

Allison, M.F. & Killham,. K. (1988) Response or soil microbial biomass to straw incorporation . Journal of Soil Science, 39, 237–242 .

Anderson, J.P.E. & Domsch, K.H. (1973): Quantification of bacterial fungal contribution to soil respiration . Archiv für Mikrobiologie, 93, 113–127 .

Anderson, J.P. E. & Domsch, K.H. (1980): Quantities of plant nutrients in the microbial biomass of selected soils . Soil Science, 130, 211–216 .

Anderson, T.H. & Domsch, K.H. (1989): Ratios of microbial biomass carbon to total organic carbon in arable soils . Soil Biology & Biochemistry, 21, 417–479 .

Anderson, T.H. & Domsch, K.H. (1993): The metabolic quotient for CO2 (qCO2) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils . Soil Biology & Biochemistry, 25, 393–395 .

Beyer, L., Blume, H.P. & Peters, M. (1991): Biological activity and organic matter transformations in typical soils of Schleswig-Holstein . Geoderma, 49, 273–284 .

Bonde, T.A & Rosswall, T. (1987): Seasonal variation of potentially mineralizable nitrogen in four cropping systems . Soil Science Society of America Journal, 51, 1508–1514 .

Brookes, P.C., Powlson, D.S. & Jenkinson D.S. (1982): Measurement of microbial biomass: phosphorus in soil . Soil Biology & Biochemistry, 14, 319–329 .

Brookes, P.C., Landman, A., Pruden, G. & Jenkinson D.S. (1985): Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial nitrogen in soil . Soil Biology & Biochemistry, 17, 837–842 .

10.

Chander, K. & Brookes, P.C. (1993): Residual effects of zinc, copper and nickel in sewage sludge on microbial biomass in a sandy loam . Soil Biology & Biochemistry, 25, 1231–1239 .

11.

Franzluebbers, A.T., Hons, F.M. & Zuberer, D.A. (1995): Soil organic carbon, microbial biomass, and mineralizable carbon and nitrogen in sorghum . Soil Science Society of America Journal, 59, 460–466 .

12.

Insam, H. and Domsch, K.H. (1988): Relationship between soil organic carbon and microbial biomass on chronosequences of reclamation sites . Microbial Ecology, 15, 177–188 .

13.

Jenkinson, D.S. (1988): Determination of microbial biomass carbon and nitrogen in soil. In: Wilson, J.R. (ed.) Advances in Nitrogen Cycles in Agricultural Ecosystems, pp. 368–386. CAB International, Wallingford, UK .

14.

Jenkinson, D.S., Davidson, S.A. & Powlson, D.S. (1979): Adenosine triphosphate and microbial biomass in soil . Soil Biology & Biochemistry, 11, 521–527 .

15.

Jenkinson, D.S. & Ladd, J.N. (1981): Microbial biomass in soil measurement and turnover. In: Paul, E.A. and Ladd, J.N. (eds) Soil Biochemistry, Vol 5, pp. 415–471. Marcel Decker, New York, USA .

16.

Lundgren, B. (1981): Fuorescent diacetate as a stain of metabolically active bacteria in soil . Oikos, 6, 17–22 .

17.

Nannipieri, P., Grego, S. & Ceccanti, B. (1990): Ecological significance of the biological activity in soil . Soil Biology Biochemistry, 6, 293–355 .

18.

Nicolardot, B., Chaussod, R. & Catroux, G. (1982): Revue des principales méthodes disponibles pour mesurer la biomasse microbienne et ses activités . Science du Sol, 4, 253–261 .

19.

Ocio, J.A. and Brookes, P.C. (1990): An evaluation of methods wheat straw and the characterization of the biomass that develops . Soil Biology & Biochemistry, 22, 685–694 .

20.

Perrott, K.W., Sarathchandra, S.U. & Dow, B.W. (1992): Seasonal and fertilizer effects on the organic cycle and microbial biomass in a hill country soil under pasture . Australian Journal of Soil Research, 30, 383–394 .

21.

Ross, D.J. (1987): Soil microbial biomass estimated by the fumigation-incubation procedure: seasonal fluctuation and influence of soil moisture content . Soil Biology & Biochemistry, 19, 397–404 .

22.

Shen, S.M., Hart, P.B.S. & Jenkinson, D.S. (1989): The nitrogen cycle in the brad balk weat experiment: 15N labeled fertilizer residues in soil microbial biomass . Soil Biology & Biochemistry, 21, 529–533 .

23.

Sneh, G., Mishra, M.M., Hooda, I.S. & Singh R. (1992): Organic mattermicrobial biomass relationships in field experiment under tropical condition: effects of inorganic fertilization and organic amendments . Soil Biology & Biochemistry, 24, 1081–1084 .

24.

Sparling, G.P. & West, A. (1988): A direct extraction method to estimate soil microbial C: calibration in situ using microbial respiration and 14C-labelled cells . Soil Biology & Biochemistry, 20, 337–343 .

25.

Tate, R.L. (1995): The soil ecosystem: biological participants. In: Soil Microbiology, pp. 30–63. John Wiley and Sons, Inc, New York .

26.

Tate, K.R.Ross, D.J. & Feltham, C.W. (1988): A direct extraction method to estimate soil microbial C: effects of experimental variables and some different calibration procedures . Soil Biology & Biochemistry, 20, 329–335 .

27.

Van Soest, P.J. & Wine, R.H. (1967): Use of detergents in the analysis of fibrous feeds. IV determination of plant cell wall constituents . Journal of AOAC, 50, 50–55 .

28.

Vance, E.D., Brookes P.C. & Jenkinson D.S. (1987a): An extraction method for measurement soil microbial biomass C . Soil Biology & Biochemistry, 19, 703–707 .

29.

Vance, E.D., Brookes, P.C. & Jenkinson, D.S. (1987b): Microbial biomass in forest soils: Determination or KC values and tests of hypotheses to explain the failure of chloroforme-fumigation-incubation method in acid soils . Soil Biology & Biochemistry, 19, 689–696 .

30.

Verstraete, W.H., Van De Werf, H., Kucnirowicz, K. & Ilaiwi, I. (1983): Specific measurement of soil microbial ATP . Soil Biology & Biochemistry, 15, 391–396 .

31.

Vischetti, C., Perucci, P. & Scarponi, L. (2000): Relationship between rimsulfuron degradation and microbial biomass content in a clay loam soil . Biology and Fertility of Soils, 31, 310–314 .

32.

Walkley, A. & Black, I.A. (1934): An examination of the degtijarf method for determination of soil organic matter and proposed modification of the chromic acid titration method . Soil Sciences, 37, 29–38 .

33.

Zuberer, D.A. (1994): Recovery and enumeration of viable bacteria. In: Methods of Soil Analysis, Part 2. Microbiological and Biochemical Properties, Book Series No. 5, pp. 119–144. Soil Science Society of America, Madison, WI, USA .

Microbial Biomass in a Soil Amended with Different Types of Organic Wastes

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