Periodontal disease (Pd) is characterized by an increased osteoclast resorption and a decreased osteoblast (OB) bone formation. OBs obtained from alveolar bone of Periodontitis patients (Pp) undergo apoptosis in the presence of TNF-related apoptosis-inducing ligand (TRAIL). We studied the intracellular apoptotic pathway induced by TRAIL; TRAIL death (DR4, DR5) and decoy (DcR1, DcR2) receptors expression in Periodontitis patients' OBs (PpOBs), and we measured the concentration of TRAIL in the serum of Pp. We demonstrated that DNA fragmentation and activation of caspase-8 and caspase-3 in PpOBs, following TRAIL stimulation, occurred in shorter time; moreover, a higher amount of both caspases was activated in order to direct OBs. Down-regulation of DcR2 in PpOBs was demonstrated and high TRAIL levels were detected in the serum of Pp. In conclusion, our data suggest that PpOBs are more sensitive to TRAIL-induced apoptosis when compared to the control group. The down-regulation of DcR2 possibly leads to an imbalanced ratio between death and decoy receptors. Our findings highlight a role of TRAIL in the pathogenesis of Pd.
D'OrlandoOGriGCattaruzziGMerluzziSBettoEGatteiVPucilloC. Outside inside signalling in CD40-mediated B cell activation. J Biol Regul Homeost Agents2007; 21:49–62.
7.
KatsanosGSAnogeianakiAOrsoCTetèSSaliniVAntinolfiPLSabatinoG. Substance P: An inflammatory peptide. Eur J Inflamm2008; 6:59–64.
8.
DeviriEGlenvilleBE. Inflammatory response in infective endocarditis. Eur J Inflamm2007; 5:57–63.
9.
MundyGR. Bone remodelling and its disorders1999 chapter 1 second edition Martin Dunitz UK p. 1.
10.
TortoriciSMauroABurruanoFDifalcoPLeoneAGerbinoABuscemiMContiPMastrangeloFTeteS. Matrix metalloproteinase-2 matrix metalloproteinase-9 and inducible nitric oxide synthase in oral leukoplakia: Immunohistochemistry and RT-PCR analysis. J Biol Regul Homeost Agents2008; 22:125–30.
11.
GiganteATorciantiMBoldriniEManzottiSFalconeGGrecoFMattioli-BelmonteM. Vitamin K and D association stimulates in vitro osteoblast differentiation of fracture site derived human mesenchymal stem cells. J Biol Regul Homeost Agents2008; 22:35–44.
12.
AlexanderEHRiveraFAMarriottI. Staphylococcus aureus - induced tumor necrosis factor - related apoptosis and caspase-8 activation in infected osteoblasts. BMC Microbiol2003; 3:5–15.
13.
VenturaMTGelardiMDi GioiaRBuquicchioRAccetturaGTummoloRAArsioniA. Statistical evaluation and parameters of phlogosis in patients sensitized to cypress. J Biol Regul Homeost Agents2007; 21:41–47.
14.
LobertoJCMartinsCA Paivados SantosSS Ferreira. Staphylococcus spp in the oral cavity and periodontal pockets of chronic periodontitis patients. Br J Microbiol2004; 35:64–68.
15.
PaolantonioMDolciMPerfettiGSammartinoGD'ArchivioDSpotoGCiampoliCDe AmicisDTetèS. Effect of a subgingival chlorhexidine chip on the clinical parameters and the levels of alkaline phosphatase activity in gingival crevicular fluid during the non-surgical treatment of periodontitis. J Biol Regul Homeost Agents2008; 22:63–72.
16.
PanGO'RourkeKChinnaiyanAM. The receptor for the cytotoxic ligand TRAIL. Science1997; 276:111–13.
17.
BocchiettoEPaolucciCBredaDSabbioniEBurasteroSE. Human monocytoid THP-1 cell line versus monocyte-derived human immature dendritic cells as in vitro models for predicting the sensitising potential of chemicals. Int J Immunopathol Pharmacol2007; 20:259–65.
18.
PannoneGSanguedolceFDe MariaS. Cyclooxygenase isozymes in oral squamous cell carcinoma: A real-time RT-PCR study with clinic pathological correlations. Int J Immunopathol Pharmacol2007; 20:317–24.
19.
PanGNiJWeiY-F. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science1997; 277:815–18.
20.
SheridanJPMarstersSAPittiRM. Control of TRAIL-induced apoptosis by a family of signalling and decoy receptors. Science1997; 277:818–21.
21.
LechardeurDDrzymalaLSharmaM. Determinants of the nuclear localization of the heterodimeric DNA fragmentation factor (ICAD/CAD). J Cell Biol2000; 150:321–34.
22.
ChiorinoGGrandM MelloScatoliniMOstanoP. From single gene to integrative molecular concept MAPS: Pitfalls and potentials of microarray technology. J Biol Regul Homeost Agents2008; 22:7–16.
LiQMorimotoKKobayashiM. A forest bathing trip increases human natural killer activity and expression of anti-cancer proteins in female subjects. J Biol Regul Homeost Agents2008; 22:45–55.
25.
StassiGCascioAIariaCGazzaraDCostaGBIannelloDArenaA. Modulation of GRO-α and TNF-α production by peripheral blood mononuclear cells treated with killed Helicobacter pylori. Eur J Inflamm2007; 5:83–7.
26.
AtkinsGJBouralexisSEvdokiouA. Human Osteoblasts are resistant to Apo2L/TRAIL-mediated apoptosis. Bone2002; 4:448–56.
27.
CapodiferroSScullyCFicarraG. Orofacial granulomatosis: Report of two cases with gingival onset. Eur J Inflamm2007; 5:51–6.
28.
MoriG.BrunettiGColucciS, Alteration of activity and survival of osteoblasts obtained from human periodontitis patients: Role of TRAIL. J Biol Regul Homeost Agents2007; 21:105–14.
29.
RaffaelliLSeriniSPiccioniE. N-3 Polyunsaturated fatty acid effect in periodontal disease: State of art and possible mechanisms involved. Int J Immunopathol Pharmacol2008; 21:261–6.
30.
LöeH. The gingival index, plaque index and the retention index systems. J Periodontol1967; 38:610–6.
31.
SilnessJLöeH. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scandinavica1964; 22:112–35.
32.
BennDK. Review of the reliability of radiographic measurements in estimating alveolar bone changes. J Clin Periodontol1990; 17:14–21.
33.
LangNPHillRG. Radiographs in periodontics. J Clin Periodontol1977; 4:16–28.
34.
HaoCBeguinotFCondorelliGTrenciaAvan MeirEGYongVWParneyIFRoaWHPetrukKC. Induction and intracellular regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mediated apoptosis in human malignant glioma cells. Cancer Res2001; 61:1162–70.
35.
KinaneDFLappinDF. Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand2001; 59:154–60.
