Genetic Variability among Blastoschizomyces Capitatus Isolates from Different Clinical Sources

Abstract

Sixteen clinical isolates and nine ATCC reference strains of Blastoschizomyces capitatus were analysed genetically, examined for the cellobiose, arbutin and salicin assimilation and tested for the aspartyl-proteinase secretion. The restriction endonuclease analysis (REA) with HpaII and HinfI enzymes and the electrophoretic karyotype (EK) were investigated. Both the restriction enzymes revealed two groups (I, II) constituted by the same isolates: 17 isolates (68%) in group I and 8 (32%) in group II. The EK analysis revealed sixteen groups. These data prompts for a genetic variability of the isolates of Blastoschizomyces capitatus and their account in two distinct genetic groups as suggested by REA. This grouping was confirmed by examing the utilisation of cellobiose, arbutin and salicin. The tests for secretory aspartyl proteinase (Sap) were positive only for three isolates, suggesting a marginal role of this specific enzyme in pathogenesis for these isolates.

Keywords

blastoschizomyces capitatus REA (Restriction endonucleases Analysis)EK (electrophoretic karyotype)carbon compounds utilisation Sap (secretory aspartyl proteinase)

References

Hazen

K.C.

1995. New and emerging yeast pathogens. Clin. Microbiol. Rev. 8:462

Perfect

J.R.

Schell

W.A.

, 1996. The new fungal opportunists are coming. Clin. Infect. Dis. 22(S):112.

Martino

Salavert

Parody

Tomas

J.F.

de la Camara

Vazquez

Jarque

Prieto

Sastre

J.L.

Gadea

Peman

Sierra

, 2004. Blastoschizomyces capitatus infection in patents with leukemia: Report of 26 cases. Clin. Infect. Dis. 38:335.

Salkin

I.F.

Gordon

M.A.

Samsonoff

W.A.

Rieder

C.L.

, 1982. Blastoschizomyces pseudotrichosporon, gen. et. sp. nov. Mycotaxon 14:497.

Salkin

I.F.

Gordon

M.A.

Samsonoff

W.A.

Rieder

C.L.

, 1985. Blastoschizomyces capitatus a new combination. Mycotaxon 22:375.

Polacheck

Salkin

I.F.

Kitzes-Cohen

Raz

, 1992. Endocarditis caused by Blastoschizomyces capitatus and taxonomic review of the genus. J. Clin. Microbiol. 30:2318.

Guarro J., J. Genè and A.M. Stchigel.1999. Developments in fungal taxonomy. Clin. Microbiol. Rev. 12:454.

Kurtzman

C.P.

Fell

J.W.

, 1998. In The yeast, a taxonomy study. 4th ed.

Elsevier Science BV

Amsterdam The Netherlands, p.1.

Barnett

J.A.

Payne

R.W.

Yarrow

, 2000. In Yeasts: Characteristics and identification. 3rd ed.

Cambridge University Press

UK, p. 1.

10.

Guého

De Hoog

G.S.

Smith

M.T.

Meyer

S.A.

, 1987. DNA relatedness, taxonomy and medical significance of Geotrichum capitatum. J. Clin. Microbiol. 25:1191.

11.

Smith

M.T.

Poot

G.A.

, 1998. Dipodascus capitatus, Dipodascus spicifer and Geotrichum clavatum: Genomic characterization. Antonie van Leewenhoek 74:229.

12.

Arnold

A.G.

Gribbin

De Levai

MacArtney

Slack

, 1981 Trichosporon capitatum causing recurrent fungal endocarditis. Thorax 36:478.

13.

Gemeinhardt

1965. Lungenpathogenitat von Tricosporon capitatum beim menschen Zentralbl. Bakteriol. Mikrobiol. Hyg. Ser. A. 196:121.

14.

Gemeinhardt

1967. Zur Frage da Pathogenitat des Mensken ein Beitrag zur Diagnostik der Lungenmykosen. Z. Tuberk. Erkr. Thoraxorgane. 124:190.

15.

Buchta

Zak

Kohout

Otcenasek

, 2001. Case report. Disseminated infection of Blastoschizomyces capitatus in a patient with acute myelocytic leukaemia. Mycoses 44:505.

16.

D'Antonio

Piccolomini

Fioritoni

Iacone

Betti

Fazii

Mazzoni

, 1994. Osteomyelitis and intervertebral discitis caused by Blastoschizomyces capitatus in a patient with acute leukemia. J. Clin. Microbiol. 32:224.

17.

D'Antonio

Romano

Iacone

Violante

Fazii

Pontieri

Staniscia

Caracciolo

Bianchini

Sferra

Vetuschi

Gaudio

Carruba

, 1999. Onychomycosis caused by Blastoschizomyces capitatus. J. Clin. Microbiol. 37:2927.

18.

Martino

P.M.

Venditti

Micozzi

Morace

L. G.

Polonelli

Mantovani

Petti

M.C.

Bugio

M.L.

Santini

Serra

Mandelli

, 1990. Blastoschizomyces capitatus: An emerging cause of invasive fungal disease in leukaemia patients. Rev. Infect. Dis. 12:570.

19.

Herbrecht

Liu

K.L.

Koenig

Waller

Dufour

Maloisel

Bergerat

J.P.

Oberling

, 1990. Trichosporon capitatum septicemia in immunocompromised patients. Pathol. Biol. 38:585.

20.

Naficy

A.B.

Murray

H.W.

, 1990. Isolated meningitis caused by Blastoschizomyces capitatus. J. Infect. Dis. 161:1041.

21.

Matthews

R.C.

Burnie

J.P.

Fox

Woods

Tabaqchali

, 1986. Immunoblot analysis of the serological response in invasive Trichosporon beigelii and Blastoschizomyces capitatus infections. J. Clin. Microbiol. 23:395.

22.

Pincus

D.H.

Salkin

I.F.

Hurd

N.J.

Levy

I.L.

Kemna

M.R.

, 1988. Modification of potassium nitrate assimilation test for identification of clinically important yeasts. J. Clin. Microbiol. 26:366.

23.

Van der Walt

J.P.

Yarrow

, 1984. Methods for isolation, maintenance, classification and identification of yeasts. In The yeasts: A taxonomic study. Rij

N.J.W. Kreger-Van

, 3rd ed.

Elsiever Science Publishers

Amsterdam The Netherlands, p.45.

24.

Carruba

Pontieri

De Bernardis

Martino

Cassone

, 1991. DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J. Clin. Microbiol. 29:916.

25.

Sherman

Fink

G.R.

Lawrence

T.H.

, 1978. In Methods in yeast genetics: A laboratory course manual. Ed. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y., p. 1.

26.

Sambrook

Fritsch

E.F.

Maniatis

, 1989. In Molecular cloning: A laboratory manual. 2nd ed.

Cold Spring Harbor Laboratory Press

Cold Spring Harbor N.Y., p.1.

27.

Pontieri

Gregori

Gennarelli

Ceddia

Novelli

Dallapiccola

De Bernardis

Carruba

, 1996. Correlation of SfiI macrorestriction endonuclease fingerprint analysis of Candida parapsilosis isolates with source of isolation. J. Med. Microbiol. 45:173.

28.

Vollrath

Davis

R.W.

, 1987. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res. 15:7865.

29.

Cassone

De Bernardis

Pontieri

Carruba

Girmenia

Martino

Fernandez-Rodriguez

Quindos

Ponton

, 1995. Biotype diversity of Candida parapsilosis and its relationship to the clinical source and experimental pathogenicity. J. Infect. Dis. 171:967.

30.

De Bernardis

Agatensi

Ross

K.I.

Emerson

Lorenzini

Sullivan

P.A.

, 1990. Evidence for a role for secreted aspartate proteinase of Candida albicans in vulvovaginal candidiasis J. Infect. Dis. 161:1276.

31.

Fan

J.B.

Chikashige

Smith

C.L.

Niwa

Yanagidea

Cantor

C.R.

, 1988. Construction of a NotI restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res. 17:2801.

32.

D'Antonio

Mazzoni

Icone

Violante

Capuani

M.A.

Schioppa

Romano

, 1996. Emergence of fluconazole resistant strains of Blastoschizomyces capitatus causing nosocomial infections in cancer patients. J. Clin. Microbiol. 34:753.

33.

Ito

Ishikawa

Fujii

Hattori

Konno

Kawakami

Kosakai

, 1988. Disseminated Trichosporon capitatum infection in a patient with acute leukemia. Cancer 61:585.

34.

Liu

K.L.

Herbrecht

Bergerat

G.P.

Koenig

Waller

Oberling

, 1990. Disseminated Trichosporon capitatum infection in a patient with acute leukaemia undergoing bone marrow transplantation. Bone Marrow Transplant 6:219.

35.

Oelz

Schaffner

Frick

Schaer

, 1983. Trichosporon capitatum: Thrush-like oral infection, local invasion, fungaemia and metastatic abscess formation in a leukemic patient. J. Infect. 6:183.

36.

Scherer

Stevens

D.A.

, 1987. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J. Clin. Microbiol. 25:675.

37.

Roy

Meyer

S.A.

, 1998. Confirmation of the distinct genotype groups within the form species Candida parapsilosis. J. Clin. Microbiol. 36:216.

38.

Zolan

M.E.

1995. Chromosome-length polymorphism in fungi. Microbiol. Rev. 59:686.

39.

Chu

Magee

B.B.

Magee

P.T.

, 1993. Construction of an Sfil macrorestriction map of the Candida albicans genome. J. Bacteriol. 175:6637.