Suppression of DNA Synthesis of Cultured Mesothelial Cells by Supplemented Albumin

Abstract

Mesothelial cells, harvested from rat omentum, were cultured with 10% FCS/MEM till the third passage, and then seeded at a density of 2-3x10⁴/cm² in a 96 well micro-titer plate. Both albumin and glucose were added to each culture medium at final concentrations of 0–2 g/dl and 0–90 mM, respectively. On days 2 and 3, 5-bromo-2'-deoxy-uridine (BrdU) was added and incubated for 24-h to be incorporated into cellular DNA and detected by the ELISA principle.

The lowest DNA synthesis was seen when both 2.0 g/dl of albumin and 90 mM of glucose were supplemented to the medium while the highest was obtained without albumin supplement. In the range of 0–90 mM of glucose and 0–2 g/dl of albumin, generally speaking, DNA synthesis of cultured mesothelial cells could be suppressed by either glucose or albumin, and it was greatly enhanced when both concentrations increased.