The aim of this study was to evaluate the effects of a hybrid liver support system (LSS) on cardiopulmonary function in nine healthy pigs. A hybrid LSS containing primary pig hepatocytes was connected to fully alert animals. The extracorporeal blood flow was maintained between 200-240 ml/min using a roller pump. Continuous plasma flow through the hybrid LSS was 50-60 ml/min. Hemodynamic and pulmonary gas exchange parameters were compared 1 hour before and 1 hour after connection to as well as 1 hour before and 1 hour after disconnection from the hybrid LSS. The hybrid LSS did not influence significantly hemodynamics and pulmonary gas exchange in this group of healthy and awake pigs. It can be concluded that the used LSS did not cause a cardiopulmonary effect per se and should be evaluated further concerning its function as a liver support system in an animal model of acute hepatic failure.
CampbellD.A., HamJ.M., Mc CurryK.R., LuceyM.R., TurcotteJ.G., MerionR.M.. Liver transplantation for fulminant hepatic failure.Am Surg1991; 57: 546–9.
6.
DevictorD., DesplanquesL., DebrayD.. Emergency liver transplantation for fulminant liver failure in infants and children.Hepatology1992; 16: 1156–62.
7.
ScottoJ., OpolonP., EteveJ.. Liver biopsy and prognosis in acute liver failure.Gut1973; 14: 927–33.
8.
NybergS.L., ShatfordR.A., HuW.S., CerraF.B.. Hepatocyte culture systems for artificial liver support: Implications for critical care medicine.Crit Care Med1992; 20: 1157–68.
9.
ChangT.M.S.. The role of biotechnology in bioartificial or hybrid artificial cells and organs.Biomat Artif Cells Artif Organs1988; 16: 5–6.
10.
UchinoJ., TsuburayaT., KumagaiF.. A hybrid bioartificial liver composed of multiplated hepatocyte monolayers.ASAIO Trans1988; 34: 927–7.
11.
SussmanN.L., ChongM.G., KoussayerT.. Reversal of fulminant hepatic failure using an extracorporeal liver assist device.Hepatology1992; 16: 60–6.
12.
KellyJ.H., ChongM.G., KoussayerT.. An improved model of acetaminophen-induced fulminant hepatic failure in dogs.Hepatology1992; 15: 329–35.
13.
KellyJ.H., ChongM.G., KoussayerT.. Assessment of an extracorporeal liver assist device in anhepatic dogs.Artif Organs1992; 16: 418–22.
14.
RozgaJ., WilliamsF., RoM.S.. Development of a bioartificial liver: Properties and function of a hollow-fiber module inoculated with liver cells.Hepatology1993; 17: 258–65.
15.
RozgaJ., HolzmanM.D., RoM.S.. Development of a hybrid bioartificial liver.Ann Surg1993; 217: 502–11.
16.
NybergS.L., PayneW.D., AmiotB.. Demonstration of biochemical function by extracorporeal xenohepatocytes in an anhepatic animal model.Transplant Proc1993; 25: 1944–5.
17.
NybergS.L., MannH.J., RemmelR.P., HuW.S., CerraF.B.. Pharmacokinetic analysis verifies P 450 function during in vitro and in vivo application of a bioartificial liver.ASAIO J1993; 39: M252–6.
18.
NybergS.L., PlattJ.L., ShirabeK., PayneW.D., CerraF.B.. Immunoprotection of xenocytes in a hollow fiber bioartificial liver.ASAIO J1992; 38: M463–7.
19.
GerlachJ., EnckeJ., HoleO., MüllerC., RyanC.J., NeuhausP.. Bioreactor for a larger scale hepatocyte in vitro perfusion.Transplantation1994; 58: 984–8.
20.
GerlachJ., SchnoyN., EnckeJ., SmithM.D., MüllerC., NeuhausP.. Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies.Hepatology1995; 22: 546–52.
21.
GerlachJ.C., EnkeJ., HoleO., MüllerC., CourtneyJ.M., NeuhausP.. Hepatocyte culture between three dimensionally arranged biomatrix-coated independent artificial capillary systems and sinusoidal endothelial cell co-culture compartments.Int J Artif Organs1994; 17: 301–6.
22.
GerlachJ.C., BrombacherJ., KlöppelK., SchnoyN., NeuhausP.. Comparison of four methods for mass hepatocyte isolation from pig and human livers.Transplantation1994; 57: 1318–22.
23.
BerggrenS.M.. The oxygen deficit of arterial blood caused by non-ventilated parts of the lung.Acta Physiol Scand1942; 4, Suppl. XI: 4–92.
24.
HughesH.C.. Swine in cardiovascular research.Lab Anim Sci1986; 36: 348–50.
25.
MountL.E., IngramD.L.. Pig physiology. In: The pig as a laboratory animal.London: Academic Press1971; 65–78.
26.
LundeenG., ManoharM., ParksC.. Systemic distribution of blood flow in swine while awake and during 1.0 and 1.5 MAC isoflurance anesthesia with or without 50% nitrous oxide.Anesth Analg1983; 62: 499–512.
27.
NeuhausP., BlumhardtG.. Extracorporeal liver perfusion: applications of an improved model for experimental studies of the liver.Int J Artif Organs1993; 16: 729–39.
28.
HannonJ.P.. Hemodynamic characteristics of the conscious resting pig: a brief review. In: TumblesonM.E., ed. Swine in biomedical research.New York: Plenum Press, 1986; 1341–52.
