Monocytes and polymorphonuclear leukocytes (PMN) were tested for functional integrity after exposure to flat sheet plasmapheresis equipment. Purified PMN were tested for chemiluminescence activity in response to a variety of triggers of the respiratory burst. Monocytes were assessed for their capacity to ingest erythrocytes sensitized with varying amounts of IgG antibodies. Both assays were demonstrated to be sensitive hallmarks of functional modulation. However, no functional differences were noted between phagocytes from blood collected prior to pheresis and those exposed to flat sheet plasmapheresis cylinders. These data suggest that plasmapheresis with the Au-topheresis C system does not influence the respiratory and phagocytic function of phagocytes returned back to the donor and implies that contact of phagocytes with artificial surfaces and/or their exposure to surface-activated plasma factors generated in the collection cylinder are minimal when using this plasmapheresis method.
McCarthyK., HensonP.M.Induction of lysosomal enzyme secretion by alveolar macrophages in response to the purified complement fragment C5a and C5ades-arg. J Immunol1979; 123: 2511–17.
2.
NguyenA.T., LethiasC., ZingraffJ. Hemodialysis membrane-induced activation of phagocyte oxidative metabolism detected in vivo and in vitro within micro-amounts of whole blood. Kidney Int1985; 28: 156–67.
3.
HensonP.M.Interaction of cells with immune complexes: adherence, release of constituents and tissue injury. J Exp Med1971; 134: 114s–135s.
4.
RagsdaleS.G., ArendW.P.Neutral protease secretion by human monocytes. Effect of surface-bound immune complexes. J Exp Med1979; 149: 954–68.
KurlanderR.J.Reversible and irreversible loss of Fc receptor function of human monocytes as a consequence of interaction with immunoglobulin. J Clin Invest1980; 66: 773–88.
7.
MichlJ., UnkelessJ.C., PieczonkaM.M., SilversteinS.C.Modulation of Fc receptors of mononuclear phagocytes by immobilized antigen-antibody complexes. Quantitative analysis of the relationship between ligand number and Fc receptor response. J Exp Med1983; 157: 1746–57.
8.
JohnstonR.B.Jr., LehmeyerJ.E., GuthrieL.A.Generation of superoxide anion and chemiluminescence by human-monocytes during phagocytosis and on contact with surface-bound immunoglobulin G. J Exp Med1976; 143: 1551–6.
9.
JungiT.W., von BelowG., LerchP.G., SpaethP.J.Modulation of human monocyte Fc receptor function by surface-adsorbed IgG. Immunology1987; 60: 261–8.
10.
WrightS.D., CraigmyleL.S., SilversteinS.C.Fibronectin and serum amyloid P component stimulate C3b- and C3bi-mediated phagocytosis in cultured human monocytes. J Exp Med1984; 158: 1338–43.
11.
MacGregorR.R.Granulocyte adherence changes induced by hemodialysis, endotoxin, epinephrine and glucocorticoids. Ann Intern Med1977; 86: 35–9.
12.
KellerH.U.Shape, motility and locomotor response of neutrophil granulocytes. In: Leukocyte locomotion and chemotaxis. Agents and Actions1982; 12(suppl): 54–72.
13.
DonabedianH., GallinJ.I.Deactivation of human neutrophil chemotaxis by chemoattractants; effect on receptors for the chemotactic factor f-met-leu-phe. J Immunol1981; 127: 839–44.
14.
KellerH.U., GerberH., HessM.W., CottierH.Studies on the regulation of neutrophil chemotactc response using a rapid and reliable method for measuring random migration and chemotaxis of neutrophil granulocytes. Agents and Actions1976; 6: 326–39.
15.
WeberL., PeterhansE.Stimulation of chemiluminescence in bovine polymorphonuclear leucocytes by virus-antibody complexes and by antibody-coated infected cells. Immunobiol1983; 164: 333–42.
16.
JungiT.W.A rapid and sensitive method allowing photometric determination of erythrophagocytosis by mononuclear phagocytes. J Immunol Methods1985; 82: 141–53.
17.
JungiT.W., BarandunS.Estimation of the degree of op-sonisation of homologous erythrocytes by IgG for intravenous and intramuscular use. Vox Sang1985; 49: 9–19.
18.
JungiT.W., HafnerS.Quantitative assessment of Fc receptor expression and function during in vitro differentiation of human monocytes to macrophages. Immunology1986: 58: 131–7.
19.
CastagnaM., TakaiY., KaibuchiK., SanoK., KikkawaV., NishizukaY.Direct activation of calcium-activated, phos-pholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem1982; 257: 7847–51.
JohnstonR.B.Jr., KitagawaS.Molecular basis for the enhanced respiratory burst of activated macrophages. Fed Proc1985; 44: 2927–32.
22.
HumesJ.L., BonneyR.J., PelusL. Macrophages syn-thesise and release prostaglandins in response to inflammatory stimuli. Nature1977; 269: 149–50.
23.
LiliusE-M, WarisM.A very sensitive and rapid chemiluminescence method for the measurement of phagocytosis. In: CrickaL.J., StanleyP.E., ThorpeG.H.G., WhiteheadT.P., eds. Analytical application of bioluminescence and chemiluminescence.New York, London: Academic Press, 1984; 401–4.
24.
KretschmerV.Leukozytenseparation und -transfusion. In: Reissigl H, Bässler KH, Hennenberg U, eds. Beiträge zur Infusionstherapie und klinischer Ernährung - Forschung und Praxis1981; 6: 1–189.
