The enzyme arginase, purified from bovine liver, was covalently immobilized by the glutaraldehyde method to the inner surface of Cuprophan hollow fibers of a conventional hemodialyzer with a surface 1.3 m3. The yield of the process was 0.3 μg/cm2 of active enzyme at physiological pH. The immobilization method did not adversely affect the physical and mechanical properties of hollow fibers neither their hemocompatibility. After sterilization with ethylene oxide, the bioreactor was able to metabolize five liters of 50 μM arginine solution at pH 7.4, in six hours.
AbuchowskiA., Van EsT., PalczukN.C., and DavisF.F.: Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol.J. Biol. Chem.252, 3578, 1977a.
2.
AbuchowskiA., McCoyJ.R., PalczukN.C., Van EsT., and DavisF.F.: Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine catalase.J. Biol. Chem.252, 3582, 1977b.
3.
AdriaenssensK., KarcherD., LowenthalA., and TerheggenH.G.: Use of enzyme-loaded erythrocytes in vitro correction of arginase-deficient erythrocytes in familial hyperargininemia.Clin. Chem.22, 323, 1976.
4.
AllisonJ.P., DavidsonL., Gutierrez-HartmanA., and KittoG.B.: Insolubilization of L-asparaginase by covalent attachment to nylon tubing.Biochem. Biophys. Res. Comm.47, 66, 1972.
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BachS.J., and SwaineD.: The effect of arginase on the retardation of tumour growth.Br. J. Cancer, 19, 379, 1965.
6.
BernarthF.R., OlanoffL.S., and ViethW.R.: Therapeutical perpectives of enzyme reactors. In: Biomedical Applications of Immobilized enzymes and proteins. Vol. 1, Ed. by ChangT.M.S., 351, Plenum Press, New York, 1977.
7.
BerüterJ., ColomboJ.P., and BachmannC.: Purification and properties of arginase from human liver and erythrocytes.Biochem. J.175, 449, 1978.
8.
CederbaumS.D., ShawK.N.F., and ValenteM.: Hyperargininaemia, J.Pediatrics, 90, 569, 1977.
9.
CederbaumS.D., ShawK.N.F., SpectorE.B., VerityM.A., SnodgrassP.J., and SugarmanG.I.: Hyperargininaemia with arginase deficiency.Pediat. Res.13, 827, 1979.
10.
CooneyD.A., WeetallH.H., and LongE.: Biochemical and Pharmacological properties of L-asparaginase bonded to Dacron vascular prostheses.Biochem. Pharm.24, 503, 1975.
11.
CurrieG.A., and BashamC.: Differential arginine dependence and the selective cytotoxic effects of activated macrophages for malignant cells in vitro.Br. J. Cancer38, 653, 1978a.
DavisF.F., AbuchowskiA., Van EsT., PalczukN.C., ChenR., SavocaK., and WiederK.: Enzyme-polyethylene glycol adducts: modified enzymes with unique properties. In: Enzyme Engineering; Vol. 4 Ed. by BrounG.B., ManeckeG., and WingardL.B.Jr., 169, 1977.
14.
GoldsteinL., and ManeckeG.: The chemistry of enzyme immobilization in: Applied biochemistry and Bioengineering; Vol. 1 Ed. by WingardL.B.Jr., Katchalski-KatzirE., and GoldsteinL., 23, Academic Press. Inc.1976.
15.
HaganJ.J., and DallanR.D.: Measurement of Arginase Activity, Anal.Biochem.22, 518. 1968.
16.
HarellD., and SokolowskyM.: Beef-liver arginase.Isolation and molecular properties. Eur. J. Biochem.25, 102, 1972.
17.
HershL.S., and WeestallH.H.: Some in vivo and in vitro studies of biologically active molecules on organic matrixes for potential therapeutic applications. In: Biomedical applications of immobilized enzymes and proteins. Vol. 1, Ed. by ChangT.M.S., 329, Plenum Press, New York, 1977.
18.
HydénH.: Enzyme Therapy and immuno-adsorption by an extracorporeal device.Biomat. Med. Dev., Art. Org., 8, 1, 1980.
19.
JacksonJ.A., HalvorsonH.R., FulrongJ.W., LucastK.D., and ShoreJ.D.: A new extracorporeal reactor-dialyzer for enzyme therapy using immobilized L-as-paraginase.J. Pharm. Exp. Ther.209, 271, 1980.
20.
JefferiesS.R., La PrestoP., BernathF.R., and JoyeuseR.: L-asparaginase bound to collagen membranes: effect of glutaraldehyde crosslinking on stabilization of catalytic activity.J. Biom. Mat. Res.12, 491, 1978.
21.
MarshallJ.J.: Manipulation of the properties of enzymes by covalent attachment of carbohydrate.Trends in Biochemical Sciences, April, 79, 1978.
22.
StorrJ.M., and BurtonA.F.: The effects of arginine deficiency on Lymphoma cells.Br. J. Cancer, 30, 50, 1974.
23.
TerheggenH.G., LowenthalA., LavinhaF., and ColomboJ.P.: Familial Hyperargininemia.Arch. Dis. In childhood, 50, 57, 1975.
