Formatted water-soluble ribbon for roll porous scaffold 3D bioprinting

Abstract

Progress of breakthrough Roll Porous Scaffold (RPS) 3D Bioprinting technology with great potential for personalized medicine, targeted therapy and overcoming the shortage of organs for implantation achieves first physical confirmation. The efficiency of RPS for the formation of solid organoid can exceed liters per hour with ~10–35 µm cells in 5, 18, and 84 pL drops due to significantly increased print exposure for cell filtration and based on the lines of many usual Kyocera inkjet printheads “KJ4B-1200,” “KJ4C-0360,” and “KJ4A-0300, KJ4B-0300” with resolution of 1200, 360, and 300 DPI accordingly. Films samples were made and a method was developed to control the formation of an object inside a roll not by one, but by two parameters. After laser perforation of these ribbons with 1200 DPI precision, holes size of ~60–120 µm and boundaries of ~20–45 µm respectively, the MTT (methylthiazolyl tetrazolium) assay with 8 replicates for each experimental group according to GOST ISO 10993-5:2023 showed good cell viability of >78%–97%. An additional parameter for formatting the reinforcing tape from a durable incompressible material, which determines the thickness of the wind layer, allows taking into account its uneven height when forming a 3D model of the organoid.