Collagen is an abundant component in the human body and plays a fundamental role in the integrity and function of various tissues, including skin, bones, joints, and connective tissues. This natural polymer also contributes to physiological balance and individual health. Within this context, this article reviews the structure of collagen, describing intrinsic characteristics that range from its molecular composition to its organization into bundles. Additionally, the review highlights some of the applications of collagen in tissue engineering, particularly its mimicry of the skin’s extracellular matrix. For this review, searches were performed in PubMed, Scopus, and Web of Sciences. The inclusion criteria were established based on the relevance of the studies for the objectives of the review and methodological quality. After selection of the articles, a critical analysis of their content was conducted and the information was synthesized and presented concisely. Analysis of the properties of collagen revealed its key importance for the design of bioactive materials in regenerative applications. However, challenges such as the need for improvement of the integration of implanted materials and a better understanding of the underlying biological processes remain.
WalkoGCastañónMJWicheG.Molecular architecture and function of the hemidesmosome. Cell Tissue Res2015; 360(3): 529–544.
2.
NyströmABruckner-TudermanL.Matrix molecules and skin biology. Semin Cell Dev Biol2019; 89: 136–146.
3.
YuJRNavarroJCoburnJC, et alCurrent and future perspectives on skin tissue engineering: key features of biomedical research, translational assessment, and clinical application. Adv Healthc Mater2019; 8(5): e1801471.
4.
GiornoLPSantosARJr. Skin: injury, repair and tissue engineering. In: BerhartLV (ed.) Advances in medicine and biology. Hauppauge, USA: Nova Science Publisher, 2022, pp. 1–85.
5.
EbrahimnejadNJaafarDGoodarziH.The past, present, future: pathophysiology, diagnosis, and treatment of human skin diseases. Physiologia2024; 4(1): 81–99.
6.
FineJDHintnerH.Life with epidermolysis bullosa (Eb).New York, USA: Springer, 2008.
ShayeganLHLevinLEGalliganER, et alSkin cleansing and topical product use in patients with epidermolysis bullosa: results from a multicenter database. Pediatr Dermatol2020; 37(2): 326–332.
9.
LimaALIllingTSchliemannS, et alCutaneous manifestations of diabetes mellitus: a review. Am J Clin Dermatol2017; 18(4): 541–553.
10.
HinesAAlaviADavisMDP. Cutaneous manifestations of diabetes. Med Clin North Am2021; 105(4): 681–697.
11.
TikhonovaIVGrinevichAATankanagAV, et alSkin microhemodynamics and mechanisms of its regulation in type 2 diabetes mellitus. Biophysics (Oxf)2022; 67(4): 647–659.
12.
LiuDNikooMBoranG, et alCollagen and gelatin. Annu Rev Food Sci Technol2015; 6: 527–557.
13.
ChaudhariAAVigKBaganiziDR, et alFuture prospects for scaffolding methods and biomaterials in skin tissue engineering: a review. Int J Mol Sci2016; 17(12): 1974.
14.
ShamsFRahimpourAVahidnezhadH, et alThe utility of dermal fibroblasts in treatment of skin disorders: a paradigm of recessive dystrophic epidermolysis bullosa. Dermatol Ther2021; 34(4): e15028.
15.
SchaferIAKovachMPriceRL, et alHuman keratinocytes cultured on collagen gels form an epidermis which synthesizes bullous pemphigoid antigens and alpha 2 beta 1 integrins and secretes laminin, type IV collagen, and heparan sulfate proteoglycan at the basal cell surface. Exp Cell Res1991; 195(2): 443–457.
16.
SundararaghavanHGMonteiroGAFiresteinBL, et alNeurite growth in 3D collagen gels with gradients of mechanical properties. Biotechnol Bioeng2009; 102(2): 632–643.
17.
SalvatoreLGalloNNataliML, et alMarine collagen and its derivatives: versatile and sustainable bio-resources for healthcare. Mater Sci Eng C Mater Biol Appl2020; 113:110963.
18.
AmirrahINLokanathanYZulkifleeI, et alA comprehensive review on collagen type I development of biomaterials for tissue engineering: from biosynthesis to bioscaffold. Biomedicines2022; 10(9): 2307.
19.
Rezvani GhomiENourbakhshNAkbari KenariM, et alCollagen-based biomaterials for biomedical applications. J Biomed Mater Res B Appl Biomater2021; 109(12): 1986–1999.
20.
Grand View Research. Collagen market size, share & trends analysis report by source (bovine, porcine), by product (gelatin, native, hydrolyzed), by application (Food & Beverages, Healthcare), and segment forecasts, 2019–2025, 2025. https://www.grandviewresearch.com/industry-analysis/native-collagen-market (accessed 07 January 2024).
21.
LanzaRLangerRVacantiJP, et al (ed.). Principles of tissue engineering. London, United Kingdom: Academic Press, 2020.
22.
RamshawJAGlattauerV. Biophysical and chemical properties of collagen: biomedical applications. Philadelphia, USA: IOP Publishing, 2019.
23.
SorushanovaADelgadoLMWuZ, et alThe collagen suprafamily: from biosynthesis to advanced biomaterial development. Adv Mater2019; 31(1): 1801651.
NaffaRMaidmentCAhnM, et alMolecular and structural insights into skin collagen reveals several factors that influence its architecture. Int J Biol Macromol2019; 128: 509–520.
26.
CreemersLBJansenIDDochertyAJ, et alGelatinase A (MMP-2) and cysteine proteinases are essential for the degradation of collagen in soft connective tissue. Matrix Biol1998; 17(1): 35–46.
27.
VelosaAPTeodoroWRYoshinariNH.Colágeno na cartilagem osteoartrótica. Rev Bras Reumatol2003; 43: 160–166.
28.
HoqueMENugeTYeowTK, et alGelatin based scaffolds for tissue engineering: a review. Polym Res J2015; 9(1): 15.
29.
GilmartinDJSoonAThrasivoulouC, et alSustained release of Cx43 antisense oligodeoxynucleotides from coated collagen scaffolds promotes wound healing. Adv Healthc Mater2016; 5(14): 1786–1799.
30.
La MonicaFCamporaSGhersiG. Collagen-based scaffolds for chronic skin wound treatment. Gels2024; 10(2): 137.
31.
HamaRReinhardtJWUlziibayarA, et alRecent tissue engineering approaches to mimicking the extracellular matrix structure for skin regeneration. Biomimetics (Basel)2023; 8(1): 130.
32.
BayerIS.Advances in fibrin-based materials in wound repair: a review. Molecules2022; 27(14): 4504.
33.
MarjubanSMHRahmanMDuzaSS, et alRecent advances in centrifugal spinning and their applications in tissue engineering. Polymers (Basel)2023; 15(5): 1253.
34.
KetharanathanV. Vascular prostheses. US Patent 4319363, 1982.
35.
HellAFSimbaraMMORodriguesP, et alProduction of fibrous polymer scaffolds for tissue engineering using an automated solution blow spinning system. Res Biomed Eng2018; 34: 273–278.
36.
ChenLZhuCFanD, et alA human-like collagen/chitosan electrospun nanofibrous scaffold from aqueous solution: electrospun mechanism and biocompatibility. J Biomed Mater Res A2011; 99(3): 395–409.
37.
GautamSChouCFDindaAK, et alSurface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Mater Sci Eng C Mater Biol Appl2014; 34: 402–409.
38.
PowellHMBoyceST.Engineered human skin fabricated using electrospun collagen-PCL blends: morphogenesis and mechanical properties. Tissue Eng Part A2009; 15(8): 2177–2187.
39.
LawJXLiauLLSaimA, et alElectrospun collagen nanofibers and their applications in skin tissue engineering. Tissue Eng Regen Med2017; 14(6): 699–718.
40.
Anaya MancipeJMBoldrini PereiraLCde Miranda BorchioPG, et alNovel polycaprolactone (PCL)-type I collagen core-shell electrospun nanofibers for wound healing applications. J Biomed Mater Res B Appl Biomater2023; 111(2): 366–381.
41.
ZhaoSLiLWangH, et alWound dressings composed of copper-doped borate bioactive glass microfibers stimulate angiogenesis and heal full-thickness skin defects in a rodent model. Biomaterials2015; 53: 379–391.
42.
SasakiYSathiGAYamamotoO.Wound healing effect of bioactive ion released from Mg-smectite. Mater Sci Eng C Mater Biol Appl2017; 77: 52–57.
43.
DhandCBalakrishnanYOngST, et alAntimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering. Int J Nanomedicine2018; 13: 4473–4492.
44.
SharifiEYousefiaslSLaderianN, et alCell-loaded genipin cross-linked collagen/gelatin skin substitute adorned with zinc-doped bioactive glass-ceramic for cutaneous wound regeneration. Int J Biol Macromol2023; 251: 125898.
45.
LoSMahmoudiEQi HaoL, et alFabrication of nanocollagen using enhanced cryogenic milling method with graphene oxide. Int J Nanomedicine2024; 19: 6845–6855.
46.
JanaSDattaPDasH, et alCopper and cobalt doped bioactive glass-fish dermal collagen electrospun mat triggers key events of diabetic wound healing in full-thickness skin defect model. J Mech Behav Biomed Mater2022; 134: 105414.
47.
DaristotleJLLauLWErdiM, et alSprayable and biodegradable, intrinsically adhesive wound dressing with antimicrobial properties. Bioeng Transl Med2019; 5(1): e10149.
48.
CarrilesJNguewaPGonzález-GaitanoG.Advances in biomedical applications of solution blow spinning. Int J Mol Sci2023; 24(19): 14757.
49.
SinghRKhanSBasuSM, et alFabrication, characterization, and biological evaluation of airbrushed gelatin nanofibers. ACS Appl Bio Mater2019; 2(12): 5340–5348.
50.
SinghRRoopmaniPHasanU, et alAirbrushed nanofibers with bioactive core and antibacterial shell for wound healing application. Eur J Pharm Biopharm2024; 195: 114169.
51.
MercanteLASchneiderRFacureM, et alEletrofiação: parâmetro e configurações de sistema, tipos de fibras e métodos de modificação. In: MercanteLACorrêaDS (orgs.), Eletrofiação e nanofibras: Fundamentos e aplicações. Capítulo 2. 2024, pp. 13–51.
52.
ZhangXXuSShenL, et alFactors affecting thermal stability of collagen from the aspects of extraction, processing and modification. J Leather Sci Eng2020; 2: 19.
53.
FujiiKKTagaYTakagiYK, et alThe thermal stability of the collagen triple helix is tuned according to the environmental temperature. Int J Mol Sci2022; 23(4): 2040.
54.
WuKLiYChenJ.Effect of pH on the structure, functional properties and rheological properties of collagen from greenfin horse-faced filefish (Thamnaconus septentrionalis) skin. Mar Drugs2024; 22(1): 45.
55.
ZhongSTeoWEZhuX, et alAn aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. J Biomed Mater Res A2006; 79(3): 456–463.
56.
AraujoTATAlmeidaMCAvanziI, et alCollagen membranes for skin wound repair: a systematic review. J Biomater Appl2021; 36(1): 95–112.
57.
SharmaSRaiVKNarangRK, et alCollagen-based formulations for wound healing: a literature review. Life Sci2022; 297: 120436.
58.
TianJFuCLiW, et alBiomimetic tri-layered artificial skin comprising silica gel-collagen membrane-collagen porous scaffold for enhanced full-thickness wound healing. Int J Biol Macromol2024; 266(Pt 1): 131233.
59.
GorellESLeungTHKhuuP, et alPurified type I collagen wound matrix improves chronic wound healing in patients with recessive dystrophic epidermolysis bullosa. Pediatr Dermatol2015; 32(2): 220–225.
