Increased Exhaled H 2 O 2 and Impaired Lung Function in Patients Undergoing Bioincompatible Hemodialysis

Abstract

Background

Chronic renal failure (CRF) and hemodialysis (HD) accumulate an inflammatory milieu, contributing to increased systemic and airway oxidative stress that may lead to lung damage.

Objectives

This study was designed to assess exhaled hydrogen peroxide (H₂O₂), lung function and whole blood chemiluminescence in HD and CRF patients and healthy controls.

Methods

The study included 59 patients (Polyamide S™ or Hemophan® membranes-19, cuprophane-16, hemodiafiltration-14, continuous ambulatory peritoneal dialysis-10), 16 CRF and 16 healthy controls. The assessment of lung function included FVC (forced vital capacity), FEV₁ (forced expiratory volume in the first second) and DLCO_c (single breath CO diffusing capacity). Exhaled H₂O₂ was determined fluorometrically and resting and n-formyl-methionyl-leucyl-phenylalanine (fMLP) luminol-dependent whole blood chemiluminescence (LBCL) were measured simultaneously.

Results

Only cuprophane HD patients presented decreased lung function (FVC 63.8±17.4%, FEV₁ 55.9±20.3 and DLCO_c 72.1± 9.3 % of predicted; p<0.05 vs. controls). These patients exhaled the highest H₂O₂ levels in comparison to CRF (p<0.01): median 0.36 μM (range R: 0.09–0.56 μM) and controls (p<0.05): 0.17 μM (0.2–17.8 μM). These levels were not decreased during the HD session: preHD 1.25 μM (0.2–16.5μM) and postHD 1.3 μM (0.2–17.8 μM). As a marker of systemic oxidative stress, fMLP-induced LBCL (total light emission) was increased in these patients (1570.6 aUxs /10phagocytes; R: 274.2–8598.9) and in the CRF group (2389.4 aUxs /10phagocytes; R: 491.5–6184; p<0.05 vs. controls). Other patient groups did not express elevated LBCL and revealed decreased exhaled H₂O₂ after a session.

Conclusions

An increased oxidative burden in the lungs may contribute to functional lung impairment in patients dialyzed with a cellulose membrane. Biocompatible dialysis with other modalities might reduce airway-borne oxidative stress and is not related with lung damage.