A Novel Bioartificial Liver Containing Small Tissue Fragments: Efficiency in the Treatment of Acute Hepatic Failure Induced by Carbon Tetrachloride in Rats
Restricted accessResearch articleFirst published online August, 2003
A Novel Bioartificial Liver Containing Small Tissue Fragments: Efficiency in the Treatment of Acute Hepatic Failure Induced by Carbon Tetrachloride in Rats
The efficiency of a new bioartificial liver (BAL) containing small tissue fragments in the treatment of acute hepatic failure induced by carbon tetrachloride in rats was evaluated. A day after injection (i.p.) of CCl4 the animals were connected to a BAL containing liver fragments (fragment BAL) and a BAL containing no liver fragments (no-fragment BAL), and extracorporeal hemoperfusion was carried out for 4 h. The activities of alanine transaminase and lactate dehydrogenase as well as the concentrations of ammonia, glucose, urea, and amino acids in plasma were measured. A tendency to the stabilisation of ammonia, glucose, phenylalanine, tyrosine, and other amino acids was revealed at the end of hemoperfusion in poisoned rats connected to the fragment BAL. A statistically significant difference in survival between the animals connected to the fragment BAL and no-fragment BAL was found. The results obtained indicate that the bioreactor containing small liver fragments is effective in the treatment of acute hepatic failure in animals.
MiyoshiH., YanagiK., FukudaH., OhshimaN.Long-term continuous culture of hepatocytes in a packed-bed reactor utilizing porous resin. Biotechnol Bioeng1994; 43: 635–44.
2.
NaruseK., NagashimaI., SakaiY.Efficacy of a bioreactor filled with porcine hepatocytes immobilized on nonwoven fabric for ex vivo direct hemoperfusion treatment of liver failure in pigs. Int J Artif Organs1998; 22: 1031–7.
3.
NybergS.L., ShirabeK., PeshwaM.V.Extracorporeal application of a gel-entrapment, bioartificial liver: demonstration of drug metabolism and other biochemical functions. Cell Transplant1993; 2: 441–52.
4.
PatzerJF2nd, MazariegosG.V., LopezR.Novel bioartificial liver support system: preclinical evaluation. Ann N Y Acad Sci1999; 875: 340–52.
5.
ShnyraA., BocharovA., BochkovaN., SpirovV.Bioartificial liver using hepatocytes on biosilon microcarriers: treatment of chemically induced acute hepatic failure in rats. Artif Organs1991; 15: 189–97.
6.
ShirahaH., KoideN., HadaH.Improvement of serum amino acid profile in hepatic failure with the bioartificial liver using multicellular hepatocyte spheroids. Biotech Bioeng1996; 50: 416–21.
7.
RozgaJ., MorsianiE., LePageE., MoscioniA.D., GiorgioT., DemetriouA.A.Isolated hepatocytes in a bioartificial liver: a single group view and experience. Biotech Bioeng1994; 43: 645–53.
8.
EllisA.J., HughesR.D., NichollD.Temporary extracorporeal liver support for severe acute alcoholic hepatitis using the Bio Logic-DT. Int J Artif Organs1999; 22: 27–34.
9.
HughesR.C., StamatoglouS.C.Adhesive interactions and the metabolic activity of hepatocytes. J Cell Sci1987; 8(suppl): S273–91.
10.
MogheP.V., BerthiaumeF., EzzellR.M., TonerM., TompkinsR.G., YarmushM.L.Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials1996; 17: 373–85.
11.
OkamotoM., IshidaY., KeoghA., StrainA.Evaluation of the function of primary human hepatocytes co-cultured with the human hepatic stellate cell (HSC) line LI90. Int J Artif Organs1998; 21: 353–9.
12.
Guguen-GuillouzoC., ClementB., BaffetG.Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res1983; 143: 47–54.
13.
NoseY., MikamiJ., KasaiY., SasakiE., AgishiT., DanjoY.An experimental artificial liver utilizing extracorporeal metabolism with sliced or granulated canine liver. Trans Am Soc Artif Intern Organs1963; 9: 358–62.
14.
SoyerT., LempinenM., WalkerJ.E., SupanwanidP., EisemanB.Extracorporeal assist of anhepatic animals with liver slice perfusion. Am J Surg1973; 126: 20–4.
15.
KoshinoI., SakamotoH., ShinadaY.Use of liver slices for an artificial liver. Trans Am Soc Artif Intern Organs1979; 25: 493–6.
16.
LieT.S., JungV., KachelF., HohnkeC., LeeK.S.Successful treatment of hepatic coma by a new artificial liver device in the pig. Res Exp Med (Berl)1985; 185: 483–94.
17.
ClaytonD.F., HarrelsonA.L., DarnellJ.E.Jr.. Dependence of liver-specific transcription on tissue organization. Mol Cell Biol1985; 5: 2623–32.
18.
PadghamC.R., BoyleC.C., WangX.J., RaleighS.M., WrightM.C., PaineA.J.Alteration of transcription factor mRNAs during the isolation and culture of rat hepatocytes suggests the activation of a proliferative mode underlies their de-differentiation. Biochem Biophys Res Commun1993; 197: 599–605.
19.
DogteromP.Development of a simple incubation system for metabolism studies with precision-cut liver slices. Drug Metab Dispos1993; 21: 699–704.
20.
SolovievV.V., OnishchenkoN.A., AkatovV.S., LezhnevE.I.Functional activity of the hepatocytes in the liver fragments in vitro: dependence on the size of fragments and on duration of their cultivation. (in Russian). Bull Eksp Biol Med1997; 124: 406–8.
21.
SolovievV.V., AkatovV.S., LezhnevE.I.Study of functional activity of the hepatocytes in the liver fragments being in the novel bioreactor “bioartificial liver”. (in Russian). Bull Eksp Biol Med2000; 129: 698–700.
22.
WangD., VerneyE., SidranskyH.Protective effect of tryptophan and cysteine against carbon tetrachloride-induced liver injury. Exp Mol Pathol1985; 43: 364–74.
23.
McIlwainH., BuddleH.L.Techniques in tissue metabolism. 1. A mechanical chopper. Biochem J1953; 53: 412–20.
24.
AkatovV.S., Ryabokon'E.N., MuratovR.M., ScopinI.I., LezhnevE.I.Study of fibroblast migration into tissue of heart valve leaflets in vitro. (in Russian). Tsitologiya2000; 42: 57–61.
25.
MikamiJ., MitoM., NashimuraA., SasakiE., NoseY., TedoI.Surgical treatment of acute liver failure. II. An experimental study of extracorporeal metabolism in the artificial liver using slices of canine liver. Jap J Gastroenterol1959; 56: 1022–7.
26.
SoyerT., LempinenM., EisemanB.In vitro extracorporal liver slices and cell suspensions for temporary hepatic support. Ann Surg1973; 177: 393–401.
27.
CatapanoG.Mass transfer limitations to the performance of membrane bioartificial liver support devices. Int J Artif Organs1996; 19: 18–35.
BarkhartK.K., HallA.H., GeraceR., RumackB.H.Hyperbaric oxygen treatment for carbon tetrachloride poisoning. Drug Saf1991; 6: 332–8.
30.
MourelleM., MezaM.A.CCl4 - induced lipoperoxidation triggers a lethal defect in the liver plasma membranes. J Appl Toxicol1990; 10: 23–7.
31.
FlendrigL.M., CaliseF., Di FlorioE.Significantly improved survival time in pigs with complete liver ischemia treated with a novel bioartificial liver. Int J Artif Organs1999; 22: 701–9.
