Ozonated autohemotherapy is used as a complementary medical approach in the treatment of vascular disorders. One of the greatest problems concerning an application of ozone in medicine is its induction of oxidative stress. The standards of ozonotherapy were elaborated recently making this treatment useful and probably non toxic. The aim of the present study was to investigate the influence of ozonated autohemotherapy on the oxidative stress extent in hemodialyzed patients, known to be particularly exposed to generation and deleterious effects of free radicals. Twelve continuously hemodialyzed subjects with atherosclerotic ischemia of the lower limbs were examined in a prospective, controlled, single blind study. Autohemotherapy with blood exposure to oxygen served as a control. The protein and lipid peroxidation products, the reduced glutathione level in red blood cells and free hemoglobin plasma concentration were measured. The study showed that ozonated autohemotherapy with ozone concentration 50 μg/ml per gram of blood induced a significant decrease in glutathione level after 9 sessions of this procedure. Therapy did not cause either the enhancement of protein and lipid peroxidation, or erythrocytes damage. It seems likely that the antioxidant defense system, part of which is glutathione, neutralizes oxidative properties of ozone in this concentration and protects against oxidative cell damage.
BocciV.Biological and clinical effects of ozone. Has ozone therapy a future in medicine? Br J Biomed Sci1999; 56: 270–9.
2.
JungersP., KhoaT.N., MassyZ.A.Incidence of atherosclerotic arterial occlusive accidents in predialysis and dialysis patients: A multicentric study in the Ile de France district. Nephrol Dial Transplant1999; 14: 898–902.
3.
SroczynskiJ., AntoszewskiZ., MatyszczykB.Clinical assessment of treatment results for atherosclerotic ischemia of the lower extremities with intraarterial ozone injections. Pol Tyg Lek1992; 47: 964–6.
4.
TylickiL., NieweglowskiT., BiedunkiewiczB., BurakowskiS., RutkowskiB.Beneficial clinical effects of ozonated autohemotherapy in chronically dialysed patients with atherosclerotic ischemia of the lower limbs – pilot study. Int J Artif Organs2001; 24: 79–82.
5.
KorenH., DevlinR., GrahamD.Ozone-induced inflammation in the lower airways of human subjects. Am Rev Respir Dis1989; 139: 407–15.
6.
HaugenE., NathK.A.The involvement of oxidative stress in the progression of renal injury. Blood Purif1999; 17: 58–65.
7.
IulianoL.The oxidant stress hypothesis of atherogenesis. Lipids2001; 36(suppl): S41–4.
8.
UenoI., HoshinoM., MiuraT., ShinrikiN.Ozone exposure generates free radicals in the blood samples in vitro. Detection by the ESR spin-trapping technique. Free Radic Res1998; 29: 127–35.
9.
MorenaM., CristolJ.P., CanaudB.Why hemodialysis patients are in a prooxidant state? What could be done to correct the pro/antioxidant imbalance. Blood Purif2000; 18: 191–9.
10.
EsterbauerH., CheesemanK.H.Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol1990; 186: 407–21.
11.
AndersonM.Enzymatic and chemical methods for the determination of glutathione. In: DolphinD., PoulsonR., AvramovicO., eds. Glutathione: Chemical, biochemical and medical aspects.John Wiley and Sons; 1989: pp. 339–65.
12.
GaribaldiS., AragnoI., OdettiP., MarinariU.M.Relationships between protein carbonyls, retinol and tocopherols level in human plasma. Biochem Mol Biol Int1994; 34: 729–36.
13.
WatkinsB., BermesE.Measurements of plasma hemoglobin. In: SundermanF., ed. Seminar on biochemical hematology.Philadelphia: Institute for Clinical Science Inc1979: pp. 57–62.
14.
HalliwellB., CrossC.E.Oxygen-derived species: their relation to human disease and environmental stress. Environ Health Perspect1994; 102: 5–12.
GirelliD., OlivieriO., StanzialA.M.Low platelet glutathione peroxidase activity and serum selenium concentration in patients with chronic renal failure: Relations to dialysis treatments, diet and cardiovascular complications. Clin Sci1993; 84: 611–7.
17.
WardR.A., McLeishK.R.Hemodialysis with cellulose membranes primes the neutrophil oxidative burst. Artif Organs1995; 19: 801–7.
18.
LonnemannG.The role of contaminated dialysate in hemodialysis-associated cytokine production. Contrib Nephrol1990; 86: 121–7.
19.
MorenaM., CristolJ.P., BoscJ.Y.Convective and diffusive losses of vitamin C during haemodiafiltration session: A contributive factor to oxidative stress in haemodialysis patients. Nephrol Dial Transplant2002; 17: 422–7.
20.
KoenigJ.S., FischerM., BulantE., TiranB., ElmadfaI., DrumlW.Antioxidant status in patients on chronic hemodialysis therapy: Impact of parenteral selenium supplementation. Wien Klin Wochenschr1997; 109: 13–9.
21.
United States Renal Data System: 1999 Annual Data Report. Am J Kidney Dis1999; 34(suppl 1): S1–176.
22.
EckardtK.U.Pathophysiology of renal anemia. Clin Nephrol2000; 53(suppl): S2–8.
23.
MiyataT., NotoyaK., YoshidaK.Advanced glycation end products enhance osteoclast-induced bone resorption in cultured mouse unfractionated bone cells and in rats implanted subcutaneously with devitalized bone particles. J Am Soc Nephrol1997; 8: 260–70.
24.
BoazM., GreenM., FainauruM., SmetanaS.Oxidative stress and cardiovascular disease in hemodialysis. Clin Nephrol2001; 55: 93–100.
25.
BiedunkiewiczB., TylickiL., NieweglowskiT.Beneficial effects of ozonated autohemotherapy in hemodialyzed patients with atherosclerotic ischemia lower limbs (abstract). Nephrol Dial Transplant2002; 17(suppl 7): S260.
26.
Rice-EvansC., MillerN.Total antioxidant status in plasma and body fluids.New York: Academic Press Inc; 1994: pp. 279–93.
27.
PryorW.A.Mechanisms of radical formation from reactions of ozone with target molecules in the lung. Free Radic Biol Med1994; 17: 451–65.
28.
HernandezF., MenendezS., WongR.Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy. Free Radic Biol Med1995; 19: 115–9.
29.
FreemanB.A., MuddJ.B.Reaction of ozone with sulfhydryls of human erythrocytes. Arch Biochem Biophys1981; 208: 212–20.
30.
ShinrikiN., SuzukiT., TakamaK.Susceptibilities of plasma antioxidants and erythrocyte constituents to low levels of ozone. Haematologia1998; 29: 229–39.
31.
BocciV., LuzziE., CorradeschiF.Studies on the biological effects of ozone: 4. Cytokine production and glutathione levels in human erythrocytes. J Biol Regul Homeost Agents1993; 7: 133–8.
32.
BocciV., ValacchiG., CorradeschiF., FanettiG.Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production. Mediators Inflamm1998; 7: 313–7.
33.
KubasovaT., HorvathM., KocsisK., FenyoM.Effect of visible light on some cellular and immune parameters. Immunol Cell Biol1995; 73: 239–44.
34.
HashimotoH., MioT., SuminoK.Lipid abnormalities of erythrocyte membranes in hemodialysis patients with chronic renal failure. Clin Chim Acta1996; 262: 137–45.
35.
CochraneC.G.Mechanisms of oxidant injury of cells. Mol Aspects Med1991; 12: 137–47.
36.
BocciV.Ozone as a bioregulator. Pharmacology and toxicology of ozonetherapy today. J Biol Regul Homeost Agents1996; 10: 31–53.
37.
BocciV., ValacchiG., CorradeschiF.Studies on the biological effects of ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone. J Biol Regul Homeost Agents1998; 12: 67–75.
38.
ZimranA., WasserG., FormanL., GelbartT., BeutlerE.Effect of ozone on red blood cell enzymes and intermediates. Acta Haematol2000; 102: 148–51.
39.
GiuntaR., CoppolaA., LuongoC.Ozonized autohemotransfusion improves hemorheological parameters and oxygen delivery to tissues in patients with peripheral occlusive arterial disease. Ann Hematol2001; 80: 745–8.
40.
GoldsteinB.D., BalchumO.J., DemopoulosH.B., DukeP.S.Electron paramagnetic resonance spectroscopy. Free radical signals associated with ozonization of linoleic acid. Arch Environ Health1968; 17: 46–9.
