Protease Removal by Means of Antiproteases Immobilized on Supports as a Potential Tool for Hemodialysis or Extracorporeal Blood Circulation

Abstract

This work studies protease concentration decrease in aqueous solutions in contact with a modified polyethersulphone graft membrane onto which antiproteases were immobilized. As a model of protease/antiprotease interaction, elastase and α₁-antitrypsin were used. Experiments were carried out either under fixed amounts of immobilized antiproteases and variable protease concentration or under fixed protease concentration and variable amounts of immobilized antiproteases. In both cases, active protease concentrations decreased with increase in contact time with the membrane. Experimental conditions under which active elastase concentration becomes zero were also found. Occurrence of the same phenomenology has also been ascertained with protease solutions obtained from human blood neutrophils. The membrane activated with α₁-antitrypsin showed differential inhibitory power on elastase and cathepsin G.

This technology could open new perspectives in manufacturing new membranes to be used in hemodialysis and extracorporeal circulation when elastase is released.

Keywords

Proteases Immobilized antiproteases Elastase Cathepsin G Human blood cells Hemodialysis Extracorporeal circulation

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