Autohemotherapy with ozone has been used for four decades with encouraging results but, owing to the lack of clinical studies, it has never been adopted by orthodox medicine. Confident of the valid principles of ozone therapy, we have endeavoured to increase its therapeutic efficacy. Over a ten-year period we have developed an apparatus that makes it possible to treat large quantities of blood with ozone in extracorporeal circulation (extracorporeal blood oxigenation and ozonation EBOO). One of us volunteered to test the system and after six treatments noted the disappearance of two lipomas. This prompted us to treat a patient with Madelung disease and several patients with atherosclerotic vasculopathy. Besides showing therapeutic effects, the preliminary results indicate that EBOO is clinically valid, without side-effects and worthy of testing in various diseases.
CrossC.E., EiserichJ.P., HalliwellB.. General biological consequences of inhaled environmental toxicants. In: CrystalR.G., WestJ.B., eds. The lung: scientific foundations.Philadelphia: Lippincott-Raven Publishers, 1997; 2421–37.
2.
PryorW.A.. Mechanisms of radical formation from reactions of ozone with target molecules in the lung.Free Radical Biol Med1994; 17: 451–65.
3.
PryorW.A., SquadritoG.L., FriedmanM.. The cascade mechanism to explain ozone toxicity: The role of lipid ozonation products.Free Rad Biol Med1995; 19: 935–41.
4.
BocciV.. Ozone as bioregulator: pharmacology and toxicology of ozone-terapy today.J Biol Regul Homeost Agents1996; 10: 31–53.
5.
BocciV.. Ozonetherapy may act as a biological response modifier in cancer. Forsch.Komplementarmed.1998; 5: 54–60.
6.
BocciV.. Ozonization of blood for the therapy of viral disease and immunodeficiencies. A hypothesis.Med Hypot1992; 39: 30–4.
7.
BocciV., LuzziE., CorradeschiF.. Studies on the biological effects of ozone: 3. An attempt to define conditions for optimal induction of cytokine.Lymphokine Cytokine Res1993; 12: 121–6.
8.
ViebahnR.. Phisikalisch-chemische, grundlagen der ozontherapie.Erfahrungsheilkunde,1975; 5: 129.
9.
HernandezF., MenendezS., WongR.. Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy.Free Rad Biol Med1995; 19: 115–9.
10.
ViebahnR.. The Use of ozone in medicine.2nd ed.Heidelberg: Karl F. Haug Publishers,1994; 1–178.
11.
WongR., MenendezS., CastanerJ.. Ozonotherapy in ischemic cardiopathy. In: Proceedings: Ozone in Medicine. 12th World Congress of the International Ozone Association, 15–18 May 1995, Lille France, edited By International Ozone Association. Tours: Instaprint, 1995; 73–7.
12.
RokitanskyO.. Klinik und Biochemie der Ozontherapie.Hospitalis,1982; 52: 643–7.
13.
SweetF., KaoM.-S, Lee S- C.D.,. Ozone selectively inhibits growth of human cancer cells.Science1980; 209: 931–3.
14.
BocciV., Di PaoloN., GarosiG.. Ozonation of blood during extracorporeal circulation. I. Rationale, methodology and preliminary studies.
15.
Van der VlietA., O'NeillC.A., EiserichJ.P., CrossC.E.. Oxidative damage to extracellular fluids by ozone and possible protective effects of thiols.Arch Biochem Biophys1995; 321: 43–50.
16.
WainerD.D.M., BurtonG.W., IngoldK.U., LockeS.. Quantitative measurement of the total, peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation.FEBS Lett1985; 187: 33–7.
17.
Effects of blood extracorporeal oxigenation and ozonation on the release of plastic substances in a new gas-eschanger.Braun Carex Laboratory (Mirandola, Italy), Internal Communication, 1–46, 1998.
18.
FreemanB.A., MuddJ.B.. Reaction of ozone with sulphydryls of human erythrocytes.Arch Biochem Biophys1981; 208: 212–20.
19.
GoldsteinB.D., BalchumO.J.. Effect of ozone on lipoid peroxidation in the red blood cell.Proc Soc Exp Biol Med1967; 126: 356–9.
20.
HuM-L. Measurement of protein thiol groups and glutathione in plasma.Methods Enzymol1994; 233: 380–5.
LeonarduzziG.. The lipid peroxidation end product 4-Hydroxy- 2,3 nonenal up-regulates transforming growth factor B1 expression in the macrofage lineage: a link between oxidative injury and fibrosclerosis.FASEB J1997; 11: 851–7.
23.
MorrowJ.D., Jackson RobertsL.. The isoprostanes: Unique bioactive products of lipid peroxidation.Prog Lipid Res1997; 36: 1–21.
24.
SerhanC.N., HaeggstromJ.Z., LeslieC.C.. Lipid mediator networks in cell signaling: Update and impact of cytokines.FASEB J1996; 10: 1147–58.
25.
BocciV., ValacchiG., CorradeschiF.. Studies on the biological effects ozone: 7. Generation of reactive oxygen species (ROS) after exposure of human blood to ozone.J Biol Regul Homeost Agents1998; 12: 67–75.
26.
ArsalaneK., GossetP., VanheeD.. Ozone stimulates synthesis of inflammatory cytokines by alveolar macrophages in vitro.Am J Respir Cell Molec Biol1995; 13: 60–8.
27.
BocciV., LuzziE., CorradeschiF.. Studies on the biological effects of ozone: 4. Cytochine production and Glutathione levels in human erythrocytes.J Biol Regul Homeost Agents1998; 12: 67–75.
28.
BocciV., PaulesuL.. Studies on the biological effects of ozone: 1. Induction of interferon gamma on human leucocytes.Haematologica1990; 75: 510–5.
29.
BocciV., ValacchiG., CorradeschiF., FanettiG.. Studies on the biological effects of ozone: 8. Effects on the total antioxidant status and on interleukin-8 production.Mediat Inflam1998; 7: 313–17.
30.
HaddadE.B., SalmonM., KotoH., BarnesP.J., AdcockI., ChungK.F.. Ozone induction of cytokine-induced neutrophil chemoattractant (CINC) and nuclear factor-kappa b in rat lung: inhibition by corticosteroids.FEBS Lett1996; 379: 265–8.
31.
JaspersI., FlescherE., ChenL.C.. Ozone-induced IL-8 expression and transcription factor binding in respiratory epithelial cells.Am J Physiol1997; 272: L504–11.
32.
LosM., DrogeW., StrickerK.. et al. Hydrogen peroxide as a potent activator of T-lymphocyte functions.Eur J Immunol1995; 25: 159–65.
33.
PaulesuL., LuzziE., BocciV.. Studies on the biologial effects of ozone: 2. Induction of tumor necrosis factors (TNF) on human leucocytes.Lymphokyne Cytokine Res1991; 10: 409–12.
34.
BocciV.. Does ozone theraphy normalize the cellular redox balance?Med Hypotheses1996; 46: 150–4.
35.
BocciV., ValacchiG., RossiD.. Studies on the biological effects of ozone: 9. Effects of ozone on human platelets.Platelets1999; 10: 110–16.
