Heat Shock Protein Hsp60 Family Shock Protein (GroEL of Escherichia coli Hsp,DnaJ of Actinobacillus Actinomycetemcomitans Hsp) May Relate PPP (pustulosis palmaris et plantaris),So By the Different Hsp Preparates We Tried to Examine the Amount of Hsp that PPP Patients Have

Abstract

Introduction

PPP is a chronic relapsing skin disease characterized by sterile intrapidermal pustules and usually scaly erythematous skin on the palm, soles or both. Tonsillitis, oral periodontal disease and metal allergy have been suspected to cause dermal diseases including PPP. From human being down to bacteria, all life has Hsp (stress protein). Because the products are caused by heat shock, it was named Hsp (Heat shock protein). Also it was produced under the situation of viral infection, heavy metal stimulation and shortage of oxygen. By the molecular weight, Hsp are classified to Hsp90, Hsp70, Hsp60 and 2600 low weight Hsp. GroEL, DnaJ belong to Hsp60 family. The oral bacterial Hsp which has strong antigenecities are very similar to Hsp of the human body. So by antibody against oral bacterial Hsp which human body products, autoimmunity disease may occur. TCR have α,β and γ,δ type. Specially palms and soles have many γ,δ T cells. These are very easy to respond to Hsp of bacteria.

Method and Result

We examined the titers of PPP patients (IgE, GroEL, DnaJ, HSV-IgG, mycoplasma pneumoniae-IgG, and Chlamydia pneumoniae-IgG) in Tokyo Dental College Chiba Hospital. There are the serum antibody levels measured by ELISA. Control is the average serum levels of people who have not PPP.

The result shows that the IgE antibody levels of PPP are not so high. The antibody levels of GroEL, DnaJ or both are higher than control or average. DanJ are Hsps of A.Actinomycetemcomitans. GroEL is Hsps of E.coli and has a high homology to gram-negative bacteria. If the antibody level is high, an infection of gram-negative bacteria is doubted. The antibody level in sera against HSV and CMV are very high in PPP patients. On both palms and sales of the patients, the strong response of CMV or HSV antibody was showed by the BiDigital O-Ring Test (OMURA, Y. 1977-2000). We made different sample of GroEL, DnaJ.

• GroEL (500pg, 1ng, 2.5ng, 5ng, 10ng, 25ng, 50ng, 75ng, 100ng)

• DnaJ (1pg, 500pg, 1ng, 2.5ng, 5ng, 7.5ng, 10ng, 25ng, 50ng, 75ng, 100ng)

By these samples, we tried to examine the amount of Hsp that PPP patients have. So treating periodontal disease for decreasing bacteria, a prescription of Chinese parsley and DHA/EPA would improve PPP disease.

Diagram 1	IgE	GroEL	DnaJ	HSV (EIA)	CMV (EIA)	mycoplasma pneumoniae(PA)	mycoplasma pneumoniae(CF)
Cut off value	< 250U/M	0.14	0.16	< 2.0	< 2.0	<40	<0.9
female	64	0.691	0.536	53.8	26.9	<40	1.28
female	33	0.451	0.231	73.3	33.4	<40	1.63
female	140	0.33	0.193	2	17.1	< 40
female	11	0.366	0.28	69.9	2	< 40
female	56	0.3	0.263	2	2	<40
female	330	0.359	0.257	11.6	35.5	<40
female	180	0.328	0.24	115	76	< 40
female	350	0.296	0.287	2	21.5	< 40
female	390	0.311	0.315	2.6	41.2	< 40
female	150	0.577	0.16	5.2	44.0	< 40	1.41
female	130			77.5	25.2	<40	0.53
female	33			2	40.8	<40	0.9

Diagram 2	GroEL	DnaJ
Control	0.1305	0.100
IWATA	0.577	0.16
KOBAYASHI	0.3375	0.17
INE	0.4355	0.217
KIKU	0.376	0.1595