Abstract
In (1) Figures 1, 4, 5 and 6 were obscured
owing to a file corruption issue. The Figures are reproduced correctly below: Calcitonin gene-related peptide (CGRP)- and glutamine synthetase (GS)-
immunoreactivity (-ir) in fresh and incubated trigeminal ganglia. (A) Numerous
nerve cell bodies showed CGRP-ir in fresh ganglia (arrows). (B) SGCs, closely
surrounding the neurons, were identified with GS (arrows). (C) GS did not
co-localize with CGRP in the satellite glial cells (arrows). (D) A large number of
neurons (arrowheads) displayed CGRP-ir after 24 hours of culture. SGC nuclei
(arrows), and other glial cells nuclei, were seemingly immunopositive for CGRP
(arrows), but this was judged to be high-background staining. (E) Arrows point at
GS-ir SGCs. (F) Merged image demonstrating GS-ir SGCs (arrows). (G–H) In some
areas, intense CGRP-ir SGCs (arrows) were found 24 and 48 hours after culture.
SGC = satellite glial cell. Demonstration of RAMP1-ir in (A) fresh and (B) cultured rat trigeminal ganglion
for 24 hours. Neurons displayed RAMP1-ir (arrows). (C) After organ culture, SGCs
exhibited RAMP1-ir (arrowheads). (D) Organ culture for 24 hours displayed enhanced
expression of RAMP1-ir in neurons. Data are given as mean ± standard error of the
mean, N = 4. Non-parametric Mann-Whitney U test was applied.
*p < .05. RAMP1-ir = receptor activity–modifying
protein1-(immunoreactivity). SGCs = satellite glial cells. Double immunohistochemistry of pERK1/2 and CGRP in fresh trigeminal ganglia (A–C)
and after 48 hours of culture (D–F). Co-localization of pERK1/2 and CGRP was found
in neurons (arrows) in fresh tissue (A–C). (D–F) Following 48 hours of culture,
co-localization was also demonstrated in SGCs (arrows) as well as in the neurons
(arrowhead). pERK1/2 = phosphorylated extracellular signal-regulated kinase 1 and
2. CGRP = calcitonin gene-related peptide. SGCs = satellite glial cells. (A) pERK1/2 staining of fresh trigeminal ganglion. (B) An enhanced expression of
pERK1/2-ir was seen in SGCs (arrowheads) and in neurons (arrows) after 24 hours of
culture. (C) Co-incubation with the MEK1/2 inhibitor U0126 (10−5 M)
markedly reduced the pERK1/2 activity both in SGCs (arrowheads) and in neurons
(arrows) at 48 hours of organ culture. (D) U0126 decreased the CGRP-ir expression
in neurons after 24 and 48 hours of culture (N = 6), and also in
the SGCs (E) at 48 hours (N = 5), but not 24 hours (data not
shown). Data are given as mean ± SEM. Non-parametric Mann-Whitney U test was
applied. **p < .01. pERK1/2 = phosphorylated extracellular
signal-regulated kinase 1 and 2. pERK1/2-ir = pERK-immunoreactivity.
SGCs = satellite glial cells. MEK1/2 = MAP kinase/ERK kinase 1/2.
CGRP-ir = calcitonin gene-related peptide-immunoreactivity.
