N-ethyl-N-nitrosourea (ENU) administered intraperitoneally or transplacentally to Sprague-Dawley (CD) and BD-IV (Berlin Druckrey IV) rats increased the incidence (26.6%) of an uncommon ovarian tumor with testicular characteristics compared either to controls (3.0%) or rats administered diethylnitrosamine (3.3%). The induced tumors were composed of tubular structures that resembled seminiferous tubules lined by Sertoli's-like cells. The abundant electron-lucent cytoplasm of the tumor cells contained polyribosomes, lipid bodies, and mitochondria but few additional organelles. Sertoli's cell-like tumors appeared to develop from the ovarian stroma in the hilar region of the ovary. They usually were benign and resulted in unilateral enlargement of the ovary. The mean serum concentrations of testosterone, estrone, and estradiol in selected rats with ovarian tumors were elevated above mean serum values in controls. There was not a consistent direct correlation between tumor diameter and circulating hormone level. Ethyl nitrosourea-induced ovarian tumors composed of testicular (Sertoli's-like) cells will provide a reproducible animal model to investigate the histogenesis and hormone secreting properties of this unique gonadal neoplasm.
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