Transmission and/or scanning electron microscopic features of leukemic cells from seven dogs with myelomonocytic leukemia, four dogs with monocytic leukemia, and three dogs with lymphocytic leukemia were studied. Few ultrastructural features distinguished normal cells from leukemic cells. Nuclear to cytoplasmic asynchrony, abnormal cytoplasmic granules, nuclear pockets, and cytoplasmic fibrillar bundles were seen more frequently in leukemic cells. Small ridges or ruffles and a few microvilli were surface characteristics of cells from dogs with myelomonocytic leukemia. An occasional cell had large undulating ruffles. Cells from dogs with monocytic leukemia and lymphocytic leukemia usually had smoother surfaces.
References
1.
BennettJ.M.CatovskyD.DanielM.T.FlandrinG.GaltonD.A.G.GralnickH.R.SultanC.(FAB Cooperative Group):
Proposals for the classification of acute leukemia.Br J Haematol33:
451–458,
1976
2.
ChapmanA.L.BoppW.S.TorresJ.Jr.CohenH.:
An electron microscopic study of the cell types in canine lymphoma and leukaemia.J Comp Pathol91:
331–340,
1981
3.
DvorakA.M.MonahanR.A.DickersinG.R.:
Diagnostic electron microscopy. I. Hematology: Differential diagnosis of acute lymphoblastic and acute myeloblastic leukemia. Use of ultrastructural peroxidase cytochemistry and routine electron microscopic technology.Pathol Annu16:
101–137,
1981
4.
GrindemC.B.StevensJ.B.PermanV.:
Morphologic classification and clinical and pathologic characteristics of spontaneous leukemia in 17 dogs.J Am Anim Hosp Assoc21:
219–227,
1985
5.
HarveyJ.W.TevellT.G.HydeD.M.JacksonR.I.:
Well-differentiated lymphocytic leukemia in a dog; long term survival without therapy.Vet Pathol18:
37–47,
1981
6.
HolmbergC.A.ManningJ.S.OsburnB.I.:
Canine malignant lymphomas: Comparison of morphologic and immunologic parameters.JNCI56:
125–135,
1976
7.
JoinerG.N.FraserC.J.JardineJ.H.TrujilloJ.M.:
A case of chronic granulocytic leukemia in a dog.Can J Comp Med40:
153–160,
1976
8.
LatimerK.S.DystraM.J.:
Acute monocytic leukemia in a dog.J Am Vet Med Assoc184:
852–854,
1984
9.
LinnabaryR.D.HolscherM.A.GlickA.D.PowellH.S.McCollumH.M.:
Acute myelomonocytic leukemia in a dog.J Am Anim Hosp Assoc14:
71–75,
1978
10.
LoebW.F.LamontP.H.CapenC.C.:
Monocytic leukemia in a dog.In:
Myeloproliferative Disorders of Animals and Man,
ed. ClarkeHowardHackett,
pp. 687–702.
USAEC Conf 680529,
Oak Ridge, Tenn.,
1970
11.
MackeyL.J.JarrettW.F.H.LauderI.M.:
Monocytic leukaemia in the dog.Vet Rec96:
27–30,
1975
12.
MeutenD.J.KocibaG.J.CapenC.C.ChewD.J.SegreG.V.LevineL.TashjianA.H.Jr.VoelkelE.F.NagodeL.A.:
Hypercalcemia in dogs with lymphosarcoma.Lab Invest49:
553–562,
1983
13.
PolliackA.:
Surface features of circulating human leukemic cells: Experience with 175 cases of leukemia examined by scanning electron microscopy.In:
Scanning Electron Microscopy/1976/II,
ed. JohariBecker,
pp. 31–40,
120.
IITRI,
Chicago,
1976
14.
RanganS.R.S.CalvertR.E.VitolsK.:
Fibrillar bundles in canine lymphomas: An ultrastructural study.J Ultrastruct Res36:
425–436,
1971
15.
SchalmO.W.JainN.C.CarrollE.J.:
Veterinary Hematology,
pp. 471–514,
3rd ed.Lea, and Febiger,
Philadelphia,
1975
16.
SeedT.M.TolleD.V.FritzT.E.DevineR.L.PooleC.M.NorrisW.P.:
Irradiation-induced erythroleukemia and myelogenous leukemia in the beagle dog: Hematology and ultrastructure.Blood50:
1061–1079,
1977
17.
ShivelyJ.N.FeldtC.DavisD.:
Fine structures of formed elements in canine blood.Am J Vet Res30:
893–905,
1969
18.
SonodaM.KobayashiK.:
Eosinophils of canine peripheral blood in electron microscopy.Jpn J Vet Res18:
43–46,
1970
19.
SonodaM.KobayashiK.:
Lymphocytes of canine peripheral blood in electron microscopy.Jpn J Vet Res18:
71–74,
1970
20.
SonodaM.KobayashiK.:
Monocytes of canine peripheral blood in electron microscopy.Jpn J Vet Res18:
67–69,
1970
21.
SonodaM.KobayashiK.:
Neutrophils of canine peripheral blood in electron microscopy.Jpn J Vet Res18:
37–41,
1970
22.
SonodaM.KobayashiK.:
Plasmacytoid cells of canine peripheral blood in electron microscopy.Jpn J Vet Res18:
125–129,
1970
23.
TolleD.V.SeedT.M.FritzE.T.LombardL.S.PooleC.M.NorrisW.P.:
Acute monocytic leukemia in the irradiated beagle.Vet Pathol16:
243–254,
1979
24.
WeberA.BinghamC.PomeroyK.:
Correlation of blood lymphocyte nuclear pocket prevalence with enzootic bovine leukemia p24 viral antibody titers.Am J Vet Res42:
1395–1399,
1981
25.
YounessE.TrujilloJ.M.AhernM.J.McCredieK.M.CorkA.:
Acute unclassified leukemia. A clinicopathologic study with diagnostic implications of electron microscopy.Am J Hematol9:
79–88,
1980
