Morphological Lesions in Red Blood Cells from Herring Gulls and Atlantic Puffins Ingesting Prudhoe Bay Crude Oil

Abstract

Red blood cells from nestling herring gulls and Atlantic puffins that had ingested 10 ml or more of a Prudhoe Bay crude oil/kg body weight/day for four to five days were examined by light and electron microscopy. In stained smears, red blood cells from oil-dosed birds were characterized by anisocytosis, poikilocytosis, reticulocytosis, and Heinz body formation. In transmission electron micrographs, affected cells had intracytoplasmic and intranuclear Heinz bodies, a variety of abnormal cytoplasmic vesicles, degenerate mitochondria, absence of circumferential microtubules, abnormal shape, and crenulation of the plasma membrane. The latter two cell surface anomalies were evident in scanning electron micrographs. Identical lesions were present in red cells from gulls injected with phenylhydrazine. Reticulocytosis was the only change evident in blood from gulls made anemic by hemorrhage. These observations support the hypothesis that the toxicity of ingested Prudhoe Bay oil to red cells was exerted by oxidant chemical compounds.