Histologic, histochemical, and electron microscopic studies of generalized ceroid-lipofuscinosis in a cynomolgus monkey are presented. Histologically, a wide variety of tissue cells contained numerous bright eosinophilic intracytoplasmic granules that varied in size from 0.5 μm to 4.0 μm in diameter. Histochemically, the granules gave a weakly positive reaction with periodic acid-Schiff and for lipids. They were weakly acid fast and capable of emitting autofluorescence. Ultrastructurally, the granules were single unit membrane-bound, and contained dense osmiophilic material with frequent concentric or fingerprint-type lamellar formation. The granules were different than hemofuscin, iron, and bilirubin. Tinctorially the granules were unique—they were bright red with hematoxylin and eosin and, thus, differed from typical age-related lipofuscin pigment.
References
1.
ArmstrongD.KoppangN.:
Ceroid-lipofuscinosis, a model for aging. In:
Age Pigments,
ed.
Sohal,
pp. 355–382.
Elsevier/North-Holland Biomedical Press,
Amsterdam,1981
2.
BjörkerudS.:
Isolated lipofuscin granules - a survey of a new field. In:
Advances in Gerontological Research,
ed.
Strehler,
pp. 257–288.
Academic Press,
New York,1964
3.
BjörkerudS.:
Studies of lipofuscin granules of human cardiac muscle. II. Chemical analysis of the isolated granules.Exp Mol Pathol3:
377–389,
1964
4.
BrizzeeK. R.OrdvJ. M.KaackB.:
Early appearance and regional differences in intraneuronal and extraneuronal lipofuscin accumulation with age in the brain of a non-human primate (Macaca mulatto).J Gerontol29:
366–381,
1974
5.
DekkerA.OehrleJ. S.:
Hyaline globules of the adrenal medulla of man.A product of lipid peroxidation? Arch Pathol91:
353–364,
1971
6.
EndicottK. M.LillieR. D.:
Ceroid, the pigment of dietary cirrhosis of rats. Its characteristics and its differentiation from hemofuscin.Am J Pathol20:
149–153,
1944
7.
HartroftW. S.PortaE. A.:
Ceroid.Am J Med Sci250:
324–345,
1965
8.
HendleyD. D.MildvanA. S.ReporterM. C.StrehlerB. L.:
The properties of isolated human cardiac age pigment. II. Chemical and enzymatic properties.J Gerontol18:
144–150,
1963
9.
HowesE. L.Jr.PriceH. M.BlumbergJ. M.:
The effects of a diet producing lipochrome pigment (ceroid) on the ultrastructure of skeletal muscle in the rat.Am J Pathol45:
599–631,
1964
10.
LillieR. D.AshburnL. L.SebrellW. H.DaftF. S.LowryJ. V.:
Histogenesis and repair of the hepatic cirrhosis in rats produced on low protein diets and preventable with choline.Public Health Rep57:
502–508,
1942
11.
LillieR. D.DaftF. S.SebrellW. H.:
Cirrhosis of liver in rats on deficient diet and effect of alcohol.Public Health Rep56:
1255–1258,
1941
12.
LunaL. G.:
Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology,
pp. 1–258.
McGraw-Hill Book Company,
New York,1968
13.
MalkoffD. B.StrehlerB. L.:
The ultrastructure of isolated and in situ human cardiac age pigment.J Cell Biol16:
611–616,
1963
14.
PearseA. G. E.:
Histochemistry, Theoretical and Applied,
pp. 1076–1085.
The Williams & Wilkins Company,
Baltimore,1972
15.
ReynoldsE. S.:
The use of lead citrate at high pH as electronopaque stain in electron microscopy.
J Cell Biol17:
208,
1963
16.
RiisR. C.SheffyB. E.LoewE.KernT. J.SmithJ. S.:
Vitamin E deficiency retinopathy in dogs.Am J Vet Res42:
74–86,
1981
17.
SamorajskiT.OrdyJ. M.KeefeJ. R.:
The fine structure of lipofuscin age pigment in the nervous system of aged mice.J Cell Biol26:
779–795,
1965
18.
SiakotosA.GoebelH.PatelV.WatanabeI.ZemanW.:
The morphogenesis and biochemical characteristics of ceroid isolated from cases of neuronal ceroid-lipofuscinosis. In:
Sphingolipids, Sphingolipidoses and Allied Disorders,
ed.
VolkAronson,
pp. 53–61.
Plenum,
New York,1972
19.
SiakotosA.WatanabeI.SaitoN.FleischerS.:
Procedures for the isolation of two distinct lipopigments from human brain: lipofuscin and ceroid.Biochem Med4:
361–375,
1970
20.
StrehlerB. L.:
On the histochemistry and ultrastructure of age pigment. In:
Advances in Gerontological Research,
ed.
Strehler,
pp. 343–384.
Academic Press,
New York,1964
21.
StrehlerB. L.MarkD. D.MildvanA. S.GeeM. V.:
Rate and magnitude of age pigment accumulation in the human myocardium.J Gerontol14:
430–439,
1959
