Well-differentiated lymphocytic leukemia was found during routine hematologic examination of a 12-year-old female poodle with signs of anxiousness, panting and pica. The total white blood cell count was 106,900/μ1 and at least 90% of the leukocytes were small, normal-appearing lymphocytes. The dog was examined several times during a 23-month period before it was killed at the owner's request for conditions unrelated to the leukemia. The hematologic picture of the leukemia was essentially unchanged during this time. A deep nuclear cleft was seen in most lymphocytes examined by electron microscopy. Cell surface markers showed most blood lymphocytes to be B-cells. These cells responded poorly to mitogen stimulation.
References
1.
AisenbergA.C.BlochK.J.:
Immunoglobulins on the surface of neoplastic lymphocytes.N Engl J Med287:
272–276,
1972
2.
AltmanN.H.SquireR.A.:
Diagnosis of canine lymphoma.J Am Vet Med Assoc157:
1676–1680,
1970
3.
AnderssonJ.MöllerG.SjobergO.:
Selective induction of DNA synthesis in T- and B-lymphocytes.Cell Immunol4:
381–393,
1972
BraundK.G.EverettR.M.AlbertR.A.:
Neurologic manifestations of monoclonal IgM gammopathy associated with lymphocyte leukemia in a dog.J Am Vet Med Assoc172:
1407–1410,
1978
6.
BrouetJ.C.ValensiF.DanielM.T.FlandrinG.PreudhommeJ.L.SeligmannM.:
Immunological classification of acute lymphoblastic leukemias: evaluation of its clinical significance in a hundred patients.Br J Haematol33:
319–328,
1976
7.
ByrdW.J.von BoehmerH.RoseB.T.:
The role of the thymus in maturational development of phytohemagglutinin and pokeweed mitogen responsiveness.Cell Immunol6:
12–24,
1973
8.
DuttaS.K.NovillaM.N.BumgardnerM.K.InglingA.:
Lymphocyte responsiveness to mitogens and quantitation of T- and B-lymphocytes in canine malignant lymphoma.Am J Vet Res39:
455–458,
1978
9.
GerberJ.D.BrownA.L.:
Effect of development and aging on the response of canine lymphocytes to phytohemagglutinin.Infect Immun10:
695–699,
1974
10.
HanT.:
Studies of correlation of lymphocyte response to phytohemagglutinin with the clinical and immunological status in chronic lymphocytic leukemia.Cancer31:
280–285,
1973
11.
HolmbergC.A.ManningJ.S.OsburnB.I.:
Malignant lymphoma with B-lymphocyte characteristics in dogs.Am J Vet Res38:
1877–1879,
1977
12.
JanossyG.GreavesM.F.:
Lymphocyte activation. I. Response of T- and B-lymphocytes to phytomitogens.Clin Exp Immunol9:
483–498,
1971
13.
JayaswalU.RoathS.HydeR.D.ChisholmD.M.SmithJ.L.:
Blood lymphocyte surface markers and clinical findings in chronic lymphoproliferative disorders.Br J Haematol37:
207–215,
1977
14.
KongshavnP.A.L.GoldP.ShusterJ.ColquhounB.FreedmanS.D.:
Ability of anti-brain heteroantisera to distinguish thymus derived lymphocytes in various species.Clin Immunol Immunopathol3:
1–15,
1974
15.
LohrmannH.-P.NovikovsL.GrawR.G.Jr.:
Cellular interactions in the proliferative response of human T and B lymphocytes to phytomitogens and allogenic lymphocytes.J Exp Med139:
1553–1567,
1974
16.
MacEwenE.G.HurvitzA.I.HayesA.:
Hyperviscosity syndrome associated with lymphocytic leukemia in three dogs.J Am Vet Med Assoc170:
1309–1312,
1977
17.
MathieuO.ClaassenH.WeibelE.R.:
Differential effect of glutaraldehyde and buffer osmolality on cell dimensions. A study on lung tissue.J Ultrastruct Res63:
20–34,
1978
18.
MaziaD.SchattenD.SaleW.:
Adhesion of cells to surfaces coated with polylysine.J Cell Biol66:
198–200,
1975
19.
OnionsD.:
B- and T-cell markers on canine lymphosarcoma cells.J Natl Cancer Inst59:
1001–1006,
1977
20.
OwenL.N.BostockD.E.BettonG.R.OnionsD.E.HomesJ.YoxallA.GormanN.:
The role of spontaneous canine tumors in the evaluation of the aetiology and therapy of human cancers.J Small Anim Pract16:
155–162,
1975
PeifferR.L.JerajK.MehlhoffT.O'LearyT.P.:
Lymphosarcoma: small lymphocyte type with ocular manifestations in a dog.Canine Pract3:
50–54,
1976
23.
PincusS.BiancoC.NuzzenzweigV.:
Increased proportion of complement-receptor lymphocytes in the peripheral blood of patients with chronic lymphocytic leukemia.Blood40:
303–310,
1972
24.
PolliackA.:
Normal, Transformed and Leukemic Leukocytes. A Scanning Electron Microscopic Atlas,
pp. 1–35,
112–126.
Springer-Verlag,
New York,
1977
25.
RundlesR.W.:
Chronic lymphocytic leukemia.In:Hematology,
ed. WilliamsBeutlerErslevRundles,
pp. 1002–1020,
2nd ed.McGraw-Hill,
New York,
1977
26.
SchalmO.W.JainN.C.CarrollE.J.:
Veterinary Hematology,
pp. 506–507,
3rd ed.Lea and Febiger,
Philadelphia,
1975
27.
ShadduckJ.A.ReddyL.LawtonG.FreemanR.:
A canine cutaneous lymphoproliferative disease resembling mycosis fungoides in man.Vet Pathol15:
716–724,
1978
28.
ShifrineM.TaylorN.J.RosenblattL.S.WilsonF.D.:
Comparison of whole blood and purified canine lymphocytes in a lymphocyte stimulation microassay.Am J Vet Res39:
687–690,
1978
29.
SonodaM.KobayashiK.:
Lymphocytes in canine peripheral blood in electron microscopy.Jpn J Vet Res18:
71–74,
1970
30.
SquireR.A.BushM.MelbyE.C.NealyL.M.YarbroughB.:
Clinical and pathologic study of canine lymphoma: clinical staging, cell classification and therapy.J Natl Cancer Inst51:
565–572,
1973
31.
TaylorD.W.SiddiquiW.A.:
Responses of enriched populations of feline T and B lymphocytes to mitogen stimulation.Am J Vet Res38:
1969–1971,
1977
32.
TerrellT.G.HolmbergC.A.OsburnB.I.:
Immunologic surface markers on nonhuman primate lymphocytes.Am J Vet Res38:
503–507,
1977
33.
ToivanenP.ToivanenA.:
Selective activation of chicken T lymphocytes by concanavalin A.J Immunol111:
1602–1603,
1973
34.
WeberW.T.:
Direct evidence for the response of B and T cells to pokeweed mitogen.Cell Immunol9:
482–487,
1973
35.
WeberW.T.:
The in vitro response of chicken B cell populations to lipopolysaccharide.J Immunol111:
1277–1280,
1973
36.
WintrobeM.M.LeeG.R.BoggsD.R.BithellT.C.AthenJ.W.FoersterJ.:
Clinical Hematology,
pp. 1432–1449,
1520–1534,
7th ed.Lea and Febiger,
Philadelphia,
1974
37.
ZanderA.R.BoopelamN.EpsteinR.B.:
Surface markers on canine lymphocytes.Transplant Proc7:
369–373,
1975
