The avian reovirus “UM 1–203” originally isolated in the United States from chickens with tenosynovitis was pathogenic for the newborn chick infected by parenteral inoculation. It induced plurivisceral lesions, which became particularly intense in the liver. The intense local multiplication of the virus provoked a necrotizing hepatitis; viral titers were maintained around an E.I.D.50 of 108/0.2 ml of organ suspension in chicks killed between the 3rd and 5th days after inoculation. As a specific cellular response to the viral multiplication, numerous polykaryocytes formed and increased in number, then regressed and disappeared from the hepatic parenchyma. By histologic and electron microscopic examination at progressive times after infection, the virus was recognizable in the polykaryocyte cytoplasm. In chicks that survived longer (killed the 5th and 6th days after inoculation), regeneration of the hepatic parenchyma occurred and seemed to develop from groups of dense hepatocytes that either lacked the virus or survived the acute phase of infection.
References
1.
BeckerF.F.LaneB.P.:
Regeneration of the mammalian liver. I. Auto-phagocitosis during dedifferentiation of the liver cell in preparation for cell division.
Am J Pathol47:
783–801,
1965.
2.
CarboniA.CervioG.CessiD.LodettiE.MandelliG.ValeriA.:
Studien über eine Virusarthritis (tendo-synovitis) in Italien. 5. Internationaler Kongress World Vet.
Poultry Assoc,
München,
Band II,
1185–1188,
1973.
3.
CarboniA.CervioG.CessiD.LodettiE.LodriniE.MandelliG.ValeriA.:
Studies on viral arthritis (teno-synovitis) in Italy.
Avian Pathol4:
87–96,
1975.
4.
CastelliG.RampinT.ValeriA.:
Osservazioni sulla infezione sperimentale del pulcino neonato con virus della tenosinovite.
Clin Vet (Milano)98:
426–430,
1975.
5.
CessiD.LombardiniF.:
Orientamenti sulla profilassi dell'artrite virale aviare.
Clin Vet (Milano)98:
414–417,
1975.
DeshmukhD.R.SavedH.I.PomeroyB.S.:
Avian reoviruses. 4. Relationship to human reoviruses.
Avian Dis13:
16–22,
1969.
10.
DeshmukhD.R.DuttaS.K.PomeroyB.S.:
Avian reoviruses. 5. Studies of ultrastructural morphology by electron microscopy.
Avian Dis15:
588–595,
1971.
11.
DuttaS.K.PomeroyB.S.:
Isolation and characterization of an enterovirus from baby chicks having an enteric infection. I. Isolation and pathogenicity.
Avian Dis11:
1–8,
1967.
12.
DuttaS.K.PomeroyB.S.:
Isolation and characterization of an enterovirus from baby chicks having an enteric infection. 2. Physical and chemical characteristics and ultrastructure.
Avian Dis11:
9–14,
1967.
13.
FellugaB.BaroniA.PetekM.:
Osservazioni ultrastrutturali su cellule di Hamster (BHK 21/13) infettate con un reovirus aviare.
Boll Soc Ital Patol10:
21–28,
1967/68.
14.
FellugaB.PetekM.BaroniA.:
Cytopathology of two mammalian cell lines infected with an avian reovirus.
J Submicros Cytol6:
159–178,
1974.
15.
JohnsonD.C.:
Diagnosis, pathology and etiology of tenosynovitis in broilers and broiler breeders.
Avian Dis16:
1067–1072,
1972.
16.
KawamuraH.ShimizuF.MaedaN.TsubaharaH.:
Avian reovirus: its properties and serological classification.
Natl Inst Anim Health Q (Tokyo)5:
115–124,
1965.
17.
LombardiD.:
Ricerche sulla immunità al virus dell'artrite virale del pollo.
Clin Vet (Milano)98:
117–123,
1975.
18.
MandelliG.PetekM.BertocchiD.:
Infezione sperimentale da virus di Crawley in pulcini neonati: aspetti morfopatologici.
Clin Vet (Milano)92:
233–243,
1969.
19.
MargolisG.KilhamL.:
Pathogenesis of intrauterine infection in rats due to reovirus type 3. 2. Pathologic and fluorescent antibody studies.
Lab Invest28:
605–613,
1973.
20.
OlsonN.O.:
Viral arthritisinDiseases of Poultry,
pp. 756–760.
Iowa State University Press,
Ames.
1972.
21.
OlsonN.O.WeissR.:
Similarity between arthritis virus and Fahey-Crawley virus.
Avian Dis16:
535–540,
1972.
22.
OuchterlonyO.:
Handbook of Immunodiffusion and Immunoelectrophoresis,
5th ed,
p. 215;
Ann Arbor, London.
1971.
23.
PapadimitriouJ.M.:
Electron micrographic features of acute murine reovirus hepatitis.
Am J Pathol47:
565–585,
1965.
24.
PetekM.BorghiG.FellugaB.BaroniA.:
II virus di Crawley: un reovirus.
Vet Ital17:
3–7,
1966.
25.
PetekM.BorghiG.:
Ciclo di crescita di un reovirus aviare.
Atti Soc Ital Sci Vet20:
736–739,
1966.
26.
PetekM.:
Studi sul reovirus di Crawley.
Atti Soc Ital Sci Vet21:
888–891,
1967.
27.
PetekM.FellugaM.BorghiG.BaroniA.:
The Crawley agent: an avian reovirus.
Arch gesamte Virusforsch21:
413–424,
1967.
28.
PetekM.D'AprileP.N.:
II test di riduzione delle placche nello studio degli anticorpi verso il reovirus dell'artrite virale.
Clin Vet (Milano)98:
418–422,
1975.
29.
PosteG.:
Virus-induced polykaryocytosis and the mechanism of cell fusion.
Adv Virus Res16:
303–356,
1970.
30.
ReedL.T.MuenchH.:
A simple method of estimating 50 per cent end points.
Am J Hyg27:
493–497,
1938.
31.
RossiC.BeccariaE.GuardaF.:
La tenosinovite virale dei polli in Piemonte.
Ann Fac Med Vet Torino20:
3–18,
1973.
32.
SahuS.P.OlsonN.O.:
Comparison of the characteristics of avian reovirus isolated from the digestive and respiratory tract, with viruses isolated from the synovia.
Am J Vet Res36:
847–850,
1975.
33.
Van der HeideL.:
Infectious tenosynovitis in poultry: diagnosis and possible control. 5.
Internationaler Kongress World Vet Poultry Assoc,
München,
Band II,
1196–1197,
1973.
34.
Van der HeideL.:
Infectious tenosynovitis: immunization experiments with a viral isolate.
Clin Vet (Milano)98:
409–413,
1975.
35.
VielitzE.:
1974, quoted by Woernle,
1974(reference 38 below).
36.
ViraghS.BartókI.:
An electron microscopic study of the regeneration of the liver following partial hepatectomy.
Am J Pathol49:
825–839,
1966.
37.
WaltersM.N.I.JoskeR.A.LeakP.J.StanleyN.F.:
Murine infection with reovirus. I. Pathology of the acute phase.
Br J Exp Pathol44:
427–436,
1936.
38.
WoernleH.BrunnerA.KussmaulK.F.:
Nachweis aviärer Reo-Viren im Agar-Gel-Präzipitationtest.
Tierärztl Umsch29:
307–312,
1974.
