Pigs on nutritional studies developed heterotopic calcifications in the left atrial endocardium, submucosa of the gastric fundus and to a lesser extent in other sites. Light and electron microscopy of mineralized tissue showed early edema and connective tissue change, deposition of calcium, granulomatous reaction and repair. There was no alkaline phosphatase activity at the sites of calcification and the distribution of this enzyme activity was normal in other tissues. Increased acid phosphatase activity was demonstrated only in the lysosomes of macrophages in areas of granulomatous inflammation. Calcium and magnesium were within normal range in serum and femoral bone. The character and distribution of the lesions suggested the possibility of protracted low grade hypervitaminosis D3.
References
1.
BarkaT.AndersonP.J.:
Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler.
J Histochem Cytochem10:
741–753,
1962.
2.
BladenH.A.MartinG.R.:
Preferential mineralization of elastin in a matrix also containing collagen.
V. International congress for electron microscopy, QQ-5;
Academic Press,
New York,
1962.
3.
BurgisserH.JacquierCLeuvenbergerM.:
Hypervitaminose D chez le porc.
Schweiz Arch Tierheilk106:
714–718,
1964.
4.
CapenC.ColeC.R.HibbsJ.W.:
The pathology of hypervitaminosis D in cattle.
Pathol Vet3:
350–378,
1966.
5.
ConnH.J.DarrowM.A.EmmelV.M.:
Staining procedures used by the Biological Stain Commission,
p. 150;
Williams and Wilkins.
Baltimore,
1960.
6.
ConstantM.A.PhillipsP.H.AngevineD.M.:
The occurrence of a calcinosis syndrome in the cotton rat. II.
Pathology J Nutr47:
327–334,
1952.
7.
CullingC.F.A.:
Handbook of Histopathological and Histochemical Techniques,
p. 471,
420;
3rd ed,
Butterworth,
London,
1974.
FriendD.W.CornerA.H.KramerJ.K.G.CharltonK.M.GilkaF.SauerF.D.:
Growth, cardiopathology and cardiac fatty acids of swine fed diets containing soybean oil or low erucic acid rapeseed oil.
Can J Anim Sci55:
49–59,
1975.
11.
FriendD.W.GilkaF.CornerA.H.:
Growth, carcass quality and cardiopathology of boars and gilts fed diets containing rapeseed and soybean oils.
Can J Anim Sci55:
571–578,
1975.
12.
FriendD.W.KramerJ.K.G.CornerA.H.:
Growth and cardiopathology in boars fed rapeseed oil.
Can J Anim Sci56:
361–364,
1976.
13.
GonnermanW.A.RampW.K.ToverudS.U.:
Vitamin D, dietary calcium and parathyroid hormone interactions in chicks.
Endocrinology96:
275–281,
1975.
14.
HarringtonD.D.:
Pathologic features of magnesium deficiency in young horses fed purified rations.
Am J Vet Res35:
503–513,
1974.
15.
HeggtveitH.A.HermanL.MishraR.K.:
Cardiac necrosis and calcification in experimental magnesium deficiency. A light and electron microscopic study.
Am J Pathol45:
757–782,
1964.
16.
HuntR.D.GarciaF.G.WalshR.J.:
A comparison of the toxicity of ergocalciferol and cholecalciferol in rhesus monkeys (Macaca mulatta).J Nutr102:
975–986,
1972.
17.
JubbK.V.F.KennedyP.C.:
Pathology of Domestic Animals,
pg. 43,
2nd ed.,
Academic Press,
New York,
1970.
18.
KayeM.:
Magnesium metabolism in the rat with chronic renal failure.
J Lab Clin Med84:
536–545,
1974.
19.
KopitoL.E.:
Atomic Absorption Methods Manual, Atomic Absorption Spectroscopy in Clinical Chemistry,
vol. III,
Jarrel-Ash Division,
Fisher Scientific,
1970.
20.
KramerJ.K.G.FriendD.W.HulanH.W.:
Lipid changes in tissues of young boars fed rapeseed oil or corn oil.
Nutr Metab19:
279–290,
1975.
21.
LawsonD.E.M.:
Vitamin D: New findings on its metabolism and its role in calcium nutrition.
Proc Nutr Soc30:
47–58,
1971.
22.
LindtS.:
D.-hypervitaminotische Calcinose bei verschiedenen Tieren.
Wien Tieraerztl Mschr55:
148–164,
1968.
23.
LojdaZ.:
Uvod do histochemie enzymu. Skripta Ustavu pro dalsi vzdelavani strednich zdravotnickych pracovniku;
Brno,
1968.
24.
LostrohA.J.:
Relationship of steroid and pituitary hormones to myocardial calcification in the mouse.
Proc Soc Exp Biol Med98:
84–88,
1958.
MillerE.R.UllreyD.E.ZutautC.L.BaltzerB.V.SchmidtD.A.HoeferJ.A.LueckeR.W.:
Magnesium requirement of the baby pig.
J Nutr85:
13–20,
1965.
27.
Nutrient Requirements of Domestic Animals,
No. 2, Nutrient requirements of swine;
National Research Council,
National Academy of Sciences,
Washington, D.C.,
1973.
PondW.G.WalkerE.F.KirtlandD.:
Weight gain, feed utilization and bone and liver mineral composition of pigs fed high or normal Ca-P diets from weaning to slaughter weight.
J Anim Sci41:
1053–1056,
1975.
30.
QuartermanJ.DalgarnoA.C.AdamA.I.FellB.F.BoyneR.:
The distribution of vitamin D between the blood and the liver in the pig, and observations on the pathology of vitamin D toxicity.
Br J Nutr18:
65–77,
1964.
31.
SeeligM. S.:
Vitamin D and cardiovascular, renal and brain damage in infancy and childhood.
Ann NY Acad Sci147:
539–582.
32.
SimesenM.G.:
Calcium inorganic phosphorus, and magnesium metabolism in health and diseaseinClinical Biochemistry of Domestic Animals,
ed. CorneliusKaneko;
p. 319,
2nd ed.,
Academic Press,
New York,
1971.
33.
WassermanR.H.:
Active vitamin D-like substances in Solanum malacoxylon and other calcinogenic plants.
Nutr Rev33:
1–5,
1975.
34.
WassermanR.H.CorradinoR.A.:
Vitamin D, calcium and protein synthesis.
Vitam Horm31:
43–103,
1973.
35.
WilsonJ.W.HarrisS.G.MooreW.D.LeipoldH.W.:
Primary hyperparathyroidism in a dog.
J Am Vet Med Assoc164:
942–946,
1974.
36.
ZugibeF.T.:
Mucopolysaccharides of the arterial wall.
J Histochem Cytochem11:
35–39,
1963.
