Mice, homozygous for the motheaten gene, developed an unusual pneumonia that was the cause of natural death of mice by 7 weeks of age. Initial lesions consisted of focal accumulations of alveolar macrophages in alveoli, especially adjacent to bronchioles. Needle-like crystals formed in lysosomes of macrophages and numerous macrophages with crystals filled most alveoli in 5- to 7-weck-old mice. Although motheaten mice had lesions in other tissues and were shown by other investigators to have immunological defects, the unusual pneumonia was the only lesion severe enough to cause death.
References
1.
ArcherG.T.BlackwoodA.:
Formation of Charcot-Leyden crystals in human eosinophils and basophils and study of the composition of isolated crystals.
J Exp Med122:
173–180,
1965.
2.
AyresW.W.StarkeyN.M.:
Studies on Charcot-Leyden crystals.
Blood5:
254–266,
1950.
3.
BarkaT.AndersonP. J.:
HistochemistryinTheory, Practice, and Bibliography,
pp. 239–242;
Harper and Row,
New York,
1963.
4.
BessisM.C.:
Living Blood Cells and Their Ultrastructure,
pp. 767;
Springer-Verlag,
New York,
1973.
5.
BessisM.C.:
Ultrastructure of lymphoid and plasma cells in relation to globulin and antibody formation.
Lab Invest10:
1040–1067,
1961.
6.
BonikosD.S.BenschK.G.NorthwayW.H.:
Oxygen toxicity in the newborn.
Am J Pathol85:
623–650,
1976.
7.
BrodyA.R.CraigheadJ.E.:
Cytoplasmic inclusions in pulmonary macrophages of cigarette smokers.
Lab Invest32:
125–132,
1975.
8.
CornellR.WalkerW.A.IsselbacherK.J.:
Small intestinal absorption of horseradish peroxidase.
Lab Invest25:
42–48,
1971.
9.
CotchinE.RoeF.J.C.:
Pathology of Laboratory Rats and Mice,
pp. 848;
Blackwell,
Oxford,
1967.
10.
DaweC.J.WilliamsW.L.:
Histochemical studies of Charcot-Leyden crystals.
Anat Rec116:
53–73,
1953.
11.
DunnT.B.AndervontH.B.:
Histology of some neoplasms and some non-neoplastic lesions found in wild mice maintained under laboratory conditions.
J Natl Cancer Inst31:
873–901,
1963.
GreenE.U.:
On the occurrence of crystalline material in the lungs of normal and cancerous Swiss mice.
Cancer Res2:
210–217,
1942.
14.
GreenM.C.SchultzL.D.:
Motheaten, an immunodeficient mutant of the mouse. I. Genetics and pathology.
J Hered66:
250–258,
1975.
15.
HersH.G.VanHoofF.:
Lysosomes and Storage Diseases,
p. 666;
Academic Press,
New York,
1973.
16.
KuhnC.IIICallawayL.A.AskinF.B.:
The formation of granules in the bronchiolar Clara cells of the rat. I. Electron microscopy.
J Ultrastruct Res49:
387–400,
1974.
17.
LutznerM.A.HansenC.T.:
Motheaten: an immunodeficient mouse with markedly less ability to survive than the nude mouse in a germfree environment.
J Immunol116:
1496–1497,
1976.
18.
NicholsB.A.:
Normal rabbit alveolar macrophages. II. Their primary and secondary lysosomes as revealed by electron microscopy and cytochemistry.
J Exp Med144:
920–932,
1976.
19.
OliverC.EssnerE.:
Formation of anomalous lysosomes in monocytes, neutrophils, and eosinophils from bone marrow of mice with Chediak-Higashi syndrome.
Lab Invest32:
17–27,
1975.
20.
RabsteinL.S.PetersR.L.SpahnG.J.:
Spontaneous tumors and pathologic lesions in SWR-J mice.
J Natl Cancer Inst50:
751–758,
1973.
21.
SandersB.J.:
Animal Histology Procedures,
pp. 185;
Government Printing Office,
Washington, D. C.,
1972.
22.
SchoberR.BenschK.G.KosekJ.C.NorthwayW.H.:
On the origin of the membranous intraalveolar material in pulmonary alveolar proteinosis.
Exp Mol Pathol21:
246–258,
1974.
23.
SchultzL.D.GreenM.C.:
Motheaten, an immunodeficient mutant of the mouse. II. Depressed immune competence and elevated serum immunoglobulins.
J Immunol116:
936–943,
1976.
24.
SorokinS.P.:
A morphologic and cytochemical study on the great alveolar cell.
J Histochem Cytochem14:
884–897,
1967.
