Five cases of amebiasis were diagnosed in goldfish (Carassius auratus) from home aquariums and from a laboratory aquarium. Granulomas containing amoebae were in many organs but were most numerous in kidneys. Because there were pseudopods, food vacuoles, vesicular nucleoli and other ultrastructural characteristics of the organisms, we identified the organisms as amoebae. On the basis of mitotic stages it is possible they belong in the family Hartmannellidae.
References
1.
BowersB.KornE.D.:
The fine structure of Acanthamoeba castellanii (Neff Strain). II. Encystment.
J Cell Biol41:
786–805,
1969.
2.
CarterR.F.:
Primary amoebic meningo-encephalitis. An appraisal of present knowledge.
Trans R Soc Trop Med Hyg66:
193–213,
1972.
3.
CulbertsonC.G.SmithJ.W.CohenH.K.MinnerJ.R.:
Experimental infection of mice and monkeys by Acanthamoeba.Am J Path35:
185–197,
1959.
MartinezA.J.DumaR.J.NelsonE.C.MorettaF.L.:
Experimental Naegleria meningoencephalitis in mice. XPenetration of the olfactory mucosal epithelium by Naegleria and pathologic changes produced: a light and electron microscope study.
Lab Invest29:
121–133,
1973.
9.
MilnerR.J.:
Nosema whitei, A microsporidian pathogen of some species of Tribolium. II. Ultrastructure.
J Invertebr Pathol19:
239–247,
1972.
10.
NobleE.R.NobleG.A.:
Amebic parasites of fishes.
J Protozool13:
478–480,
1966.
11.
PageF.C.:
Taxonomic criteria for limax amoebae, with description of three new species of Hartmannella and three of Vahlkampfia.J Protozool14:
499–521,
1967.
12.
PerkinsF.O.:
Electron microscope studies of sporulation in the oyster pathogen, Minchinia costalis (Sporozoa: Haplosporida).
J Parasitol55:
897–920,
1969.
13.
PlehnM.:
Parktikum der Fischkrankheiten,
pp. 422–424;
E. Schweizerbart'sche Verlagsbuchhandlung,
Stuttgart,
1924.
14.
ReynoldsE.S.:
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
J Cell Biol17:
298–312,
1963.
15.
RichardsC.S.:
Acanthamoeba as tissue invader in freshwater mollusks.
J Protozool9(Suppl):14,
1962.
16.
RichardsC.S.:
Two new species of Hartmannella amebae infecting freshwater mollusks.
J Protozool15:
651–656,
1968.
17.
SinghB.N.:
Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order Amoebida.Philos Trans R Soc Lond236:
435–476,
1952.
18.
SpragueV.VernickS.H.:
Light and electron microscopic study of a new species of Glugea (Microsporida, Nosematidae) in the 4-spined stickleback Apeltes quadracus.J Protozool15:
547–571,
1968.
19.
VickermanK.:
Patterns of cellular organization in limax amoebae. An electron microscope study.
Exp Cell Res26:
497–519,
1962.