The ultrastructural nature of canine distemper inclusions was studied in the bladder epithelium of 4 dogs. These tissues were compared to 5 control animals. The classical intracytoplasmic inclusion consists of filaments or tubules which are helices of ribonuclcoprotein of the virus. A second type of inclusion is described and occurs in control and infected dogs. The heterogeneous dense body is not related to canine distemper infection.
References
1.
CarréH.:
Sur la maladic des jeunes chiens,
C. R. Acad. Sci.140:
669–690
(1905).
2.
CaulfieldJ.B.:
Effects of varying the vehicle for OsO4 in tissue fixation.
J. biophys. biochem. Cytol.3:
827–830
(1957).
3.
HicksR.M.:
The fine structure of the transitional epithelium of rat ureter.
J. ceel Biol.26:
25–48
(1965).
4.
KanczakN.M.KrallJ.I.HayesE.R.ElliottW.B.:
Lysosomal fractions from transitional epithelium.
J. cell Biol.24:
259–266
(1965).
5.
KoestnerA.LongJ.F.:
Ultrastructure of canine distemper virus in explant tissue cultures of canine cerebellum.
Lab. Invest.23:
196–201
(1970).
6.
LbntzO.:
Spezifische Veränderungen an den Gehirnzellen wut- und staupekranker Tiere.
Zbl. Bakt. (Abt. 1. Ref.)42:
126–127
(1909).
7.
LuftJ.H.:
Improvements in epoxy resin embedding methods.
J. biophys. biochem. Cytol.9:
409–414
(1961).
8.
MoultonJ.BrownC.H.:
Antigenicity of canine distemper inclusion bodies as demonstrated by fluorescent antibody technique.
Proc. Sec. exp. Biol. Med.86:
99
(1954).
9.
NorrbyE.FridingB.RockbornG.GardS.:
The ultrastructure of canine distemper virus.
Arch. Ges. Virttsforsch.13:
335–344
(1963).
10.
PaladeG.E.:
A study of fixation for electron microscopy.
J. exp. Med.95:
285–298
(1952).
11.
ReynoldsE.S.:
The use of lead citrate at high pH as an electron opaque stain in electron microscopy.
J. cell Biol.17:
208–212
(1963).
12.
RichterW.R.MoizeS.M.:
Electron microscopic observations on the collapsed and distended mammalian urinary bladder (Transitional epithelium).
J. ultrastruct. Res.9:
1–9
(1963).