Bacillary hemoglobinuria was expertmenially induced in rabbits by intravenous inoculation with Spores of Clostridium bemolyticum and subsequent hepatic biopsy. The biopsy wound disrupted the hepatic vasculature and damaged the adjacent parenchyma. This damage and the resulting hypoxia created conditions favorable for the germination of the inoculated spores. As the bacteria proliferated, their exotoxin lysed the hepatocytes and the erythrocytes in the immediate area. The bulk of the hepatic necrosis in bacillary hemoglobinuria was due to this exotoxin. Vascular thrombi caused by the exotoxic damage enhanced the development of the lesion.
References
1.
BoydA.G.:
Bovine bacillary hemoglobinuria.
Monthly Bulletin of the Department of Agriculture, State of California18:
534–538
(1929).
2.
CobbL.M.McKayK.A.ArchibaldJ.MarkowitzJ.:
Clostridial infection of the liver in the canine after ligation of the hepatic artery.
Canad. J. Surg.5:
450–460
(1962).
3.
HughesJ.P.:
A simplified instrument for obtaining liver biopsies in cattle.
Amer. J. vet. Res.23:
1111–1113
(1962).
4.
IversonJ.P.:
Eighth Biennial Report of the State Veterinarian of California,
pp. 8–10
(1916).
5.
MackW.B.RecordsE.:
Studies of an obscure cattle disease in western Nevada.
J. amer. vet. med. Ass.52:
143–151
(1917).
6.
MarkowitzJ.ArchibaldJ.DownieH.G.:
Experimental Surgery,
4th Ed.,
p. 850
(Williams and Wilkins,
Baltimore1959).
7.
McClungL.S.ToabeR.:
The egg-yolk plate reaction for the presumptive diagnosis of Clostridium sporogenes and certain species of the gangrene and borulinum groups.
J. Bact.53:
139–147
(1947).
8.
MeyerK.F.:
Studies to diagnose a fatal disease of cattle in the mountainous regions of California.
J. amer. vet. med. Ass.48:
552–565
(1916).
9.
OlanderH.J.HughesJ.P.BibersteinE.L.:
Bacillary hemoglobinuria: Induction by liver biopsy in naturally and experimentally infected animals.
Path. vet.3:
421–450
(1966).
10.
ParapartA.K.DzikmianA.J.:
The chemical composition of the red cell membrane.
Cold Spr. Harb. Symp. Quant, Biol.8:
17–22
(1940).
11.
RecordsE.VawterL.R.:
Bacillary hemoglobinuria of cattle and sheep.
Ner. Agr. Exp. Bul.173:
(1945).
12.
RecordsE.VawterL.R.:
Recent studies of icterohemoglobinuria of cattle.
J. amer. vet. med. Ass.68:
494–512
(1926).
13.
RousP.LarrimoreL.D.:
Relation of the portal blood to liver maintenance. A demonstration of liver atrophy conditional on compensation.
J. exp. Med.31:
609–632
(1920).
14.
SarinF.R.MillerF.R.SmithburnK.C.ThomasR.M.HummelL.E.:
Changes in the bone marrow and blood cells of developing rabbits.
J. exp. Med.64:
97
(1936).
15.
SimmonsJ.S.GentzkowC.J.:
Medical and Public Health Laboratory Methods,
p. 649
(Lea and Febiger,
Philadelphia1955).
16.
TurnerA.W.:
Black disease (infectious necrotic hepatitis) of sheep in Australia.
Bul. 46, Coun. Sci. and Ind. Res. Commonwealth Australia (quoted by Records and Vawter,
1945).
17.
TurnerA.W.EalesC.E.:
Studies on Clostridium oedematiens group; ‘H’ and ‘O’ antigenic analysis.
Austr. exp. Biol. med. Sci.21:
79
(1943).
18.
Van KampenK.R.KennedyP.C.:
Experimental bacillary hemoglobinuria. I. Intrahepatic detection of spores of Clostridium hemolylicum by immunofluorescence in the rabbit.
Amer. J. vet. Res.29:
2173–2177
(1968).
