Abstract
A porcine adenovirus isolated from the brain of a pig with encephalitis was shown to be pathogenic for germfree and pathogen-free pigs. Lesions were produced by 5 different routes of inoculation, of which the intranasal was the most effective. The animals inoculated intranasally developed an interstitial pneumonia which involved entire lobules and was especially prominent near the hilus. Distinct intranuclear inclusion bodies were seen. Lesions and inclusion bodies were also found in the kidneys, thyroids, and lymph nodes. Viral antigen was demonstrated in the lesions by immunofluorescence. Intracerebral inoculation resulted in polioencephalitis involving only the cerebral cortex. Many features of this disease were similar to adenoviral diseases of man and other animal species.