Rhode Island Red chicks were fed from hatching to 8 weeks of age a commercial ration to which was added a highly toxic groundnut meal; groups were killed at regular intervals for biochemical and histological examinations. Growth was severely retarded and the liver/body weight ratio was increased compared with control chicks. Increased hepatic fat, reduced vitamin A storage and fluctuation in the RNA/DNA ratio were also observed. These biochemical features of groundnut toxicity are discussed in relation to pathological changes.
References
1.
AllcroftR.LewisG.:
Groundnut toxicity in cattle: Experimental poisoning of calves and a report on clinical effects in older cattle.Vet. Rec.75:
487–494
(1963).
2.
AsplinF.D.CarnaghanR.B.A.:
The toxicity of certain groundnut meals for poultry with special reference to their effect on ducklings and chickens.Vet. Rec.73:
1215–1219
(1961).
3.
BrachetJ.:
In: The Nucleic Acids,
Vol. 2.
ChargaffE.DavidsonJ.N. (eds).
(Academic Press,
New York1955).
4.
ButlerW.H.BarnesJ.M.:
Toxic effects of groundnut meal containing aflatoxin to rats and guinea pigs.Brit. J. Cancer17:
699–710
(1963).
5.
CarnaghanR.B.A.LewisG.:
Unpublished data.
6.
CarrF.H.PriceE.A.:
Colour reactions attributed to vitamin A.Biochem. J.20:
497–501
(1926).
7.
DickensF.JonesH.E.H.:
The carcinogenic action of aflatoxin after its subcutaneous injection in the rat.Brit. J. Cancer17:
691–698
(1963).
8.
DischeZ.:
über einige neue charakteristische Farbreaktionen der Thymonukleinsäure und eine Mikromethode zur Bestimmung derselben in tierischen Organen mit Hilfe dieser Reaktionen.Mikrochemie8:
4–32
(1930).
9.
DischeZ.:
In: The Nucleic Acids,
Vol. 1.
ChargaffE.DavidsonJ.N. (eds).
(Academic Press,
New York1955).
10.
HardingJ.D.J.DoneJ.T.LewisG.AllcroftR.:
Experimental groundnut poisoning in pigs.Res. vet. Sci.4:
217–229
(1963).
11.
HillK.:
Comment on the histological appearances in serial liver biopsies and post-mortem specimens.Vet. Rec.75:
493–494
(1963).
12.
de IonghH.Van PeltJ.G.OrdW.O.BarrattC.B.:
A semiquantitative determination of aflatoxin B1 in groundnut meal, groundnuts and peanut butter.Vet. Rec.76:
901–903
(1964).
13.
Interdepartmental Working Party, Report:
Toxicity Associated with Certain Batches of Groundnuts. (Ministry of Agriculture, Fisheries & Food, London
1962).
14.
KingE.J.WootonI.D.P.:
Micro-analysis, in:
Medical Biochemistry,
3rd ed.
(Churchill,
London1956).
15.
LancasterM.C.JenkinsF.P.PhilpJ. McL.:
Toxicity associated with certain samples of groundnuts.Nature, Lond.192:
1095–1096
(1961).
16.
MejbaumW.:
über die Bestimmung kleiner Pentosomengen insbesondere in Derivaten der Adenylsäure.Hoppe. Seylers. Z.258:
117–120
(1939).
17.
PopperH.SchaffnerF.:
Liver: Structure and Function.
(McGraw-Hill,
New York1957).
18.
SargeantK.SheridanA.O'KellyJ.CarnaghanR.B.A.:
Toxicity associated with certain samples of groundnuts.Nature, Lond.192:
1096–1097
(1961).
19.
ShankR.C.WoganG.N.:
Effects of aflatoxin B1 on some aspects of liver metabolism.Fed. Proc.23:
200
(1964).
20.
SmithR.H.:
The influence of toxins of Aspergillus flavus on the incorporation of (C14) leucine into proteins.Biochem. J.88:
50P51P
(1963).
21.
SperryW.M.:
Lipide analysis. In:
Methods of Biochemical Analysis,
Vol. 2.
GlickD. (ed).
(Interscience,
New York1955).
22.
ThompsonS.Y.GangulyJ.KonS.K.:
The conversion of β-carotene to vitamin A in the intestine.Brit. J. Nutr.3:
50–78
(1949).
