Cytogenetic analyses have demonstrated the association of specific chromosomal changes with particular types of soft tissue tomors. This work describes the molecular cytogenetic approaches to genetic analysis of these tumors. It illustrates how molecular cytogenetics may provide a rapid and sensitive method of diagnosis and can contribute to identify specific genes implied in the aetiology of soft tissue tumors.
Dal CinP., KodsP., SciotR., De WeverI., Van DammeB., Van de VenW., Van Den BergheH.: Cytogenetic and fluorescence in situ hybridization investigation of ring chromosomes characterizing a specific pathologic subgroup of adipose tissue tumors. Cancer Genet. Cytogenet., 68: 85–90, 1993.
2.
DesmazeC., ZucmannJ., DelattreO., MelotT., ThomasG., AuriasA.: Interphase molecular cytogenetics of Ewing's sarcoma and peripheral neuroepithelioma t (11; 22) with flanking and overlapping cosmid probes. Cancer Genet. Cytogenet., 74: 13–18, 1994.
3.
GiovanniniM., SelleriL., BiegelJ.A., ScotlandK., EmanuelB.S., EvansG.A.: Interphase cytogenetics for the detection of t (11; 22) (q24; q12) in small round cell tumors. J. Clin. Invest., 90: 1911–1918, 1992.
4.
HindkjaerJ., KockJ., TerkelsenC., BrandtC.A., KolvraaS., BolundL.: Fats, sensitive multicolor detection of nucleic acids in situ by primed in situ labeling (PRINS). Cancer Genet. Cytogenet., 66: 152–154, 1994.
LeeW., HanK., HarrisC.P., ShimS., MeisnerL.F.: Use of FISH to detect chromosomal translocations and deletions. Analysis of chromosome rearrangement in synovial sarcoma cells from paraffin-embedded specimens. Am. J. Pathol., 143: 15–19, 1993.
7.
RabbittsT.H.: Chromosomal translocations in human cancer. Nature, 372: 143–149, 1994.
8.
ShipleyJ.M., ClarkJ., CrewA.J., BirdsallS., RocquesP.J., GillaS., ChellyJ., MonacoA.P., AbeS., GustersonB.A., CooperC.S.: The t (X; 18) (p11.2; q11.2) translocation found in human synovial sarcomas involves two distinct loci on the X chromosome. Oncogene, 9: 1447–1453, 1994.
9.
SreekantaiahC., LadanyiM., RodriguezE., ChagantiR.S.K.: Chromosomal aberration in soft tissue tumors. Relevance to diagnosis, classification, and molecular mechanisms. Am. J. Pathol., 144: 1121–1134, 1994.
10.
SuijkerbuijkR.F., Olde WeghuisD.E., Van Der BergM., PedeutourF., ForusA., MyklebostO., GlierC., CarelTurc, Geurts Van KesselA.: Comparative hybridization as a tool to define two distinct chromosome 12-derived amplification units in well-differentiated liposarcomas. Genes Chromosom. Cancer, 9: 292–295, 1994.
11.
TaylorC., PatelK., JonesT., KielyF., De StavolaB.L., SheerD.: Diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor based on the detection of t (11; 22) using fluorescence in situ hybridization. Br. J. Cancer, 67: 128–133, 1993.
