The axillary lymph nodes from 31 mammary carcinoma patients who had undergone radical mastectomy and were negative for metastases at routine histologic examination of hilar sections, were investigated with E29, an anti-epithelial monoclonal antibody, to detect the presence of neoplastic epithelial cells. In 4 of 433 lymph nodes examined (0.9 %) this antibody revealed the presence of epithelial metastatic foci which had not been observed at routine histological examination or interpreted as histiocytes. The 4 lymph nodes belonged to 4 different patients.
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References
1.
AltmannsbergerM., WeberK., HolscherA., SchauerA., OsbornM.: Antibodies to intermediate filaments as diagnostic tools.Lab. Invest.,46: 520–526, 1982.
2.
DelsolG., GatterK.C., SteinH., ErberW.N., PulfordK.A.F., ZinneK., MasonD.Y.: Human lymphoid cells express epithelial membrane antigen. Implications for diagnosis of human neoplasms.Lancet, II: 1124–1128, 1984.
3.
Forza Operativa Nazionale sul Carcinoma Mammario: I Tumori della Mammella.Protocollo di Anatomia Patologica,FONCAM, Milano, 1981.
4.
GatterK.C., AlcockC., HeryetA., PulfordK.A., HeydermanE., Taylor-PapadimitriouJ., SteinH., MasonD.Y.: The differential diagnosis of routinely processed anaplastic tumors using monoclonal antibodies.Am. J. Clin. Pathol.,82: 33–43, 1984.
5.
HartweitF.: The routine histological investigation of axillary lymph nodes for metastatic breast cancer.J. Pathol.,143: 187–191, 1984.
6.
JaffeE.S.: Surgical Pathology of the Lymph Nodes and Related Organs. pp. 36–38, W.B. Saunders Co., Philadelphia, 1985.
7.
NevilleA.M., MonaghanP., McllhinneyR.A.J., GustersonB., CoombesR.C.: Monoclonal antibodies in the detection and treatment of breast cancer micrometastases. In: New Concepts in Neopasia as Applied to Diagnostic Pathology.Fenoglio-PreiserC.M., WeinsteinR.S., and KaufmanN. (eds.), pp. 193–205, William and Wilkins, Baltimore, 1986.
8.
ReddingW.H., MonaghanP., ImrieS.F., OrmerodM.G., GazetJ.C., CoombesR.C., ClinkH. McD., DearnaleyD.P., SloaneJ.P., PowlesT.J., NevilleA.M.: Detection of micrometastases in patients with primary breast cancer.Lancet,2: 1271–1274, 1983.
9.
RosenP.P., SaigoP.E., BraunD.W., WeathersE., FracchiaA., KinneD.W.: Axillary micro- and macrometastases in breast cancer. Prognostic significance of tumor size.Ann. Surg.,194: 585–591, 1981.
10.
SaphirO., AmroninG.D.: Obscure axillary lymph-node metastasis in carcinoma of the breast.Cancer,1: 238–241, 1948.
11.
SloaneJ.P., OrmerodM.G., ImrieS.F., CoombesR.C.: The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections.Br. J. Cancer,42: 392–398, 1980.
12.
WeinsteinJ.N., KeenanA.M., HoltonO.D., CovellD.G., SieberS.M., BlackC.D.V., BarbetJ., TalleyM.J., ParkerR.J.: Use of monoclonal antibodies to detect metastases of solid tumors in lymph nodes.Dev. Oncol.,35: 218–232, 1985.
13.
WellsC.A., HeryetA., BrochierJ., GatterK.C., MasonD.Y.: The immunocytochemical detection of axillary micrometastases in breast cancer.Br. J. Cancer,50: 193–197, 1984.
14.
WilkinsonE.J., HauseL.: Probability in lymph node sectioning.Cancer,33: 1269–1274, 1974.
