The antigenic profile of C-26 and C-51 BALB/c colonic adenocarcinomas was examined by in vivo and in vitro assays. Mice immunized with irradiated C-26 or C-51 tumor cells from freshly excised tumor nodules or from in vitro-growing cell lines were able to reject a challenge of both tumors. Spleen lymphocytes of immune but not of normal mice were effective in cross-inhibiting tumor growth in vivo in a Winn assay. Tissue-associated antigens common to C-26 and C-51 and to their metastases but not to other syngeneic neoplasms were detected in vitro by cytotoxic T lymphocytes obtained after 5 days of a secondary culture of immune lymphocytes and irradiated tumor cells. Activated lymphocytes were obtained by exposure of spleen cells to interleukin 2 or by allostimulation. Such lymphocytes, although cytotoxic in vitro on C-26 and C-51 carcinomas, were unable to significantly reduce in vivo tumor growth in the Winn assay.
BrattainM.G., Strobel-StevensJ., FiveD., WebbM., SarrifA.M.: Establishment of mouse colonic carcinoma cell lines with different metastatic properties.Cancer Res., 40: 2142–2146, 1980.
3.
CarboneG., ColomboM.P., SensiM., CernuschiA., ParmianiG.: In vitro detection of cell-mediated immunity to individual tumor-specific antigens of chemically induced BALB/c fibrosarcomas.Int. J. Cancer, 31: 483–489, 1983.
4.
CarboneG., PierottiM.A., BoiocchiM., De LeoA.B., RadiceP., BorrelloM.G., MeseguerA., CernuschiA., ParmianiG.: Cell surface antigens of chemically induced fibrosarcomas: detection by a monoclonal antibody of a tumor-restricted Mr 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus.Cancer Res., 45: 4980–4985, 1985.
5.
CorbettT.H., GriswoldD.P., RobertB.J., JeckhamJ.C., SchabelF.M.: Tumor induction relationships in development of transplantable cancers of the colon in mice for chemotherapy assays, with a note on carcinogen structure.Cancer Res., 35: 2434–2439, 1975.
6.
De LeoA.B., ShikuH., TakahashiT., JohnM., OldL.J.: Cell surface antigens of chemically induced sarcoma of the mouse.J. Exp. Med., 146: 720–734, 1977.
7.
GiovarelliM., SantoniA., ForniG.: Alloantigenactivated lymphocytes from mice bearing a spontaneous « non-immunogenic » adenocarcinoma inhibit its growth in vivo by recruiting host immunoreactivity.J. Immunol., 133: 3596–3603, 1985.
8.
GriswoldD.P., CorbettT.H.: A colon tumor model for anticancer agent evaluation.Cancer, 36: 2441–2444, 1975.
9.
HaaseP., LowenD.M., KnowlesJ.C.: Evaluation of dimethylhydrazine-induced tumors in mice as a model system for colorectal cancer.Br. J. Cancer, 28: 530–543, 1973.
HellstromK.E., HellstromI., BrownJ.P.: Unique and common tumor-specific transplantation antigens of chemically induced mouse sarcomas.Int. J. Cancer, 21: 317–322, 1978.
13.
HurrellS.M., ZarlingY.M.: Ly-2+ effectors cytotoxic for syngeneic tumor cells: generation by allogeneic stimulation and by supernatants from mixed leukocyte cultures.J. Immunol., 131: 1017–1023, 1983.
14.
KedarE., IkejiriB.L., GorelikE., HerbermanR.B.: Natural cell-mediated cytotoxicity in vitro and inhibition of tumor growth in vivo by murine lymphoid cells cultured with T cell growth factor (TCGF).Cancer Immunol. Immunother., 13: 14–18, 1982.
15.
LawL.W.: Generation of cross-reacting tumor antigens in ascitic derivatives from murine methylcholanthrene-induced sarcomas.Int. J. Cancer, 33: 547–551, 1984.
16.
LeffelM.S., CogginJ.H.Jr.: Common transplantation antigens on methylcholanthrene-induced murine sarcomas detected by three assays of tumor rejection.Cancer Res., 37: 4112–4119, 1977.
17.
LeshemB., GotsmanB., KedarE.: In vitro elicitation of cytotoxic response against a non-immunogenic murine tumor by allosensitization.Cancer Immunol. Immunother., 17: 117–123, 1984.
18.
MacphailS., PaciucciP.A., StutmanO.: Phenotypic heterogeneity of antisyngeneic tumor killer cells generated in allogenic mixed lymphocyte reactions.J. Immunol., 132: 3204–3210, 1984.
19.
MazumderA., RosenbergS.A.: Successful immunotherapy of natural killer-resistant established pulmonary melanoma metastases by the intravenous adoptive transfer of syngeneic lymphocytes activated in vitro by interleukin 2.J. Exp. Med., 159: 495–507, 1982.
20.
MorishitaH., ShikuH., HoribeK., ObataY., StockertE., OettgenH.F., OldL.J., YamadaK.: Cell surface antigens of murine leukemias induced by radiation leukemia virus. Recognition of individually distinct cell surface antigens by cytotoxic T cells on leukemias expressing cross-reactive transplantation antigens.J. Exp. Med., 163: 452–457, 1986.
21.
MuléJ.J., EttinghausenS.E., SpiessP.J., ShuS., RosenbergS.A.: Antitumor efficacy of lymphokineactivated killer cells and recombinant interleukin-2 in vivo: survival benefit and mechanism of tumor escape in mice undergoing immunotherapy.Cancer Res., 46: 676–683, 1986.
22.
PaciucciP.A., MacphailS., ZarlingJ.M., BachF.H.: Lysis of syngeneic solid tumor cells by allo-antigen stimulated mouse T and non-T cells.J. Immunol., 124: 370–375, 1980.
23.
ParmianiG., SensiM.L., CarboneG., ColomboM.P., PierottiM.A., BallinariD., HilgersJ., HilkensJ.: Cross-reactions between tumor cells and allogeneic normal tissues. Inhibition of a syngeneic lymphoma outgrowth in H-2 and non-H-2 alloimmune BALB/c mice.Int. J. Cancer, 29: 323–332, 1982.
24.
ParmianiG., SensiM.L., BalsariA., ColomboM.P., Gambacorti-PasseriniC., GrazioliL., RodolfoM., CascinelliN., FossatiG.: Adoptive immunotherapy of cancer with immune and activated lymphocytes in experimental and clinical studies.Ric. Clin. Lab., 16: 1–20, 1986.
25.
RosensteinM., YronI., KaufmannY., RosenbergS.A.: Lymphokine-activated killer cells: lysis of fresh syngeneic natural killer-resistant murine tumor cells by lymphocytes cultured in interleukin-2.Cancer Res., 44: 1946–1953, 1984.
26.
SatoN., MichaelidesM.C., WallackM.K.: Characterization of tumorigenicity, mortality, metastasis and splenomegaly of two cultured murine colon lines.Cancer Res., 41: 2267–2272, 1981.
27.
SatoN., MichaelidesM.C., WallackM.K.: Transplantation immunity and cross-protection of two cultured murine colon lines.J. Natl. Cancer Inst., 70: 261–265, 1983.
28.
SatoN., KikuchiK.: Partial characterization of n-butanol-solubilized rejection-type antigens of syngeneic murine colon tumors.J. Natl. Cancer Inst., 74: 883–888, 1985.
29.
SensiM.L., ParenzaM., ParmianiG.: Alloreactivity and tumor antigens: generation of syngeneic anti-lymphoma killer lymphocytes by alloimmunization of mice with normal cells.J. Natl. Cancer Inst., 70: 291–297, 1983.
30.
TsuruoT., NaganumaK., YamoriT., KawabataH., Oh-haraT., IidaH., TsukagoshiS., SakuraiY.: Spontaneous metastasis of highl metastatic variants of mouse tumors and the effect of drugs on the metastasis.Gan, 75: 557–563, 1984.
31.
WinnH.J.: Immune mechanism in homotransplantation. II. Quantitative assay of the immunological activity of lymphoid cells stimulated by tumor homografts.J. Immunol., 86: 228–233, 1961.
