Bouvardin, a new antineoplastic plant product, inhibits macromolecular synthesis in P388 cells in a dose-dependent manner. At the same concentration of bouvardin, protein synthesis was inhibited to a greater extent than the synthesis of DNA and RNA. There was a reversal of inhibition of both DNA and RNA synthesis after the cells were washed free of bouvardin. However, there was partial reversal of inhibition of protein synthesis when the cells were washed free of the drug.
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References
1.
AdwankarM.K., ChitnisM.P.: Antitumour effect of extracts of Rubia cordifolia.Indian J. Exptl. Biol., 180: 102, 1980.
2.
AdwankarM.K., ChitnisM.P.: Anticancer activity of RC-18, a pure isolate from Rubia cordifolia on spectrum of experimental tumour models.Chemotherapy, 28: 291, 1982.
3.
AdwankarM.K., ChitnisM.P.: Effect of RC-18, a new anticancer principle isolated from Rubia cordifolia on cells of P388 tumour system.Biomedicine, 36: 104–105, 1982.
4.
AdwankarM.K., ChitnisM.P.: Alteration in natural resistance of sarcoma-180 tumour to bouvardin (NSC 259968) following hyperthermia.Tumori, 69: 309–312, 1983.
5.
AdwankarM.K., KhandalekarD.D., ChitnisM.P.: Combination of chemotherapy of early and advanced murine P388 leukemia with bouvardin, cis-diamminedichloroplatinum and vincristine.Oncology, 41: 370–373, 1984.
BrownF.U., KleinM., KandaswamyT.S., MandelH.G.: Rapid membrane filtration techniques for studying the action of 6-thioguanine on tumor cells in vitro.Cancer Res., 23: 254–259, 1963.
8.
ChitnisM.P., AlateA.D., MenonR.S.: Effect of bouvardin (NSC 259968) on growth characteristics of nucleic acids and protein synthesis profiles of P388 leukemia cells.Chemotherapy, 27: 126–130, 1981.
9.
GeranR.I., GreenbergN.M., MacDonaldM.M., SchumacherA.M., AbbottB.J.: Protocols for screening chemical agents and natural products against animal tumors and other biological systems.Cancer Chemother. Rep., 3: 1–87, 1972.
JohnsonR.K., ChitnisM.P.: Antineoplastic and biochemical effects in murine tumors of bouvardin cyclic hexapeptide from Bouvardia ternifloria (Rubiaceae).Proc. Am. Assoc. Cancer Res., 19: 218, 1978.
12.
MenonR.S., ChitnisM.P., AdwankarM.K., BasrurV.S., AmonkarA.J.: Tween-80 alters natural resistance of sarcoma-180 to bouvardin (NSC 259968).Tumori, 70: 23–27, 1984.
13.
SuffnessM., DourosJ.: Drugs of plant origin.Methods Cancer Res., 16: 73–126, 1979.
14.
TobeyR.A., OrlickyD.J., DeavenL.L., RallL.B., KissaneR.J.: Effects of bouvardin (NSC 259968), a cyclic hexapeptide from Bouvardia terniflora, on the progression capacity of cultured Chinese hamster cells.Cancer Res., 38: 4415–4421, 1978.
15.
UmezawaH.: Cancer drugs of microbial origin.Methods Cancer Res., 16: 43–72, 1979.
16.
ZalacainM., ZaeraE., VasquezD., JimenezA.: The mode of action of the antitumor drug bouvardin, an inhibitor of protein synthesis in eukaryotic cells.FEBS Lett., 148: 95–97, 1982.
