A correlation was obtained between a positive nitrite test in urine and the severity of urinary bacterial infection. Bacteria isolated from the urine of bilharzial or bladder cancer patients were found to be rich in nitrate reductase activity. « Escherichia coli » was the most common microorganism isolated from these specimens. Urine and several urinary constituents activate bacterial nitrate reductase. β-Glucuronidase activity in the urine of patients with chronic « Schistosoma haematobium » infection and bladder cancer was measured and shown to be significantly greater than that of urine of normal control subjects. Urinary bacterial infection was shown to be the source of the increased urinary level of enzyme activity at pH 7.0.
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References
1.
Abdel TawabG.A., El-ZoghbyS.M., Abdel SamieY.M., ZakiA.M., KhalefT.S., El-SewedyS.M.: Urinary β-glucuronidase enzyme activity in some bilharzial urinary tract diseases. Trans. R. Soc. Trop. Med. Hyg., 62: 501–505, 1968.
2.
AyanabaA., VerstraeteW., AlexanderM.: Possible microbial contribution to nitrosamine formation in sewage and soil. J. Natl. Cancer Inst., 50: 811–813, 1973.
3.
BeuhlerH.J., KatzmanP.A., DoisyP.P., DoisyE.A.: Studies on β-glucuronidase from E. coli. Proc. Soc. Exp. Biol., 72: 297–299, 1949.
4.
BolyandE., WallaceD.M., WilliamsD.C.: Activity of enzymes sulphatase and β-glucuronidase in urine serum and bladder tissue. Br. J. Cancer, 9: 62–79, 1955.
5.
DolbeyR.V., MooroA.W.: The incidence of cancer in Egypt. An analysis of 671 cases. Lancet, 1: 587, 1924.
6.
El-AaserA.A., El-MerzabaniM.M., HiggyN.A., Abdel-KaderM.M.: A study on the aetiological factors of bilharzial bladder cancer in Egypt: 3. Urinary β-glucuronidase. Eur. J. Cancer, 15: 573–583, 1979.
7.
El-SebaiI.: Cancer of bladder in Egypt. Kasr El-Aini J. Surg., 2: 183–230, 1961.
8.
FishmanW.H.: In Methods of Biochemical Analysis.GlickO. (Ed.), New York, Interscience Press, p. 77, 1967.
9.
FrippP.I.: The origin of urinary β-glucuronidase. Br. J. Cancer, 19: 330–335, 1965.
10.
GoharM.A.: Urinary sepsis in bilharziasis. Proc. 1st Int. Symp. on Bilharziasis, Part1: 569–573, 1962.
11.
HawksworthG.M., HillM.J.: Bacteria and the N-nitrosation of secondary amines. Br. J. Cancer, 25: 520–526, 1971.
12.
HillM.J., HawksworthG.M.: Bacterial production of nitrosamines in vitro and in vivo. IARC Scientific Publications No.3: 127–129, 1972.
13.
HoeprichP.D.: Culture of the urine. J. Lab. Clin. Med., 56: 899, 1960.
14.
JacoxR.F.: Streptococcal β-glucuronidase. J. Bacteriol., 65: 700, 1953.
15.
KlubesP., CarnaL., RasinowitzedF., JondorfW.R.: Factors affecting dimethylnitrosamine formation from simple precursors by rat intestinal bacteria. Food Cosmet. Toxicol., 10: 757–767, 1972.
16.
LewisF.J.W., PlaiceC.H.J.: Urinary β-glucuronidase activity in cancer of the bladder and other diseases. Br. J. Cancer, 14: 106–120, 1960.
MontgomeryH.A., DymockJ.F.: The determination of nitrite in water. Analyst, 86: 41–416, 1961.
21.
NordenD.A., GelfandM.: Bilharzial and bladder cancer. An investigation of urinary β-glucuronidase associated with S. haematobium infection. Trans. R. Soc. Trop. Med. Hyg., 66: 864, 1972.
22.
SanderJ.: Nitrosaminsynthese durch Bakterien. Hoppe-Seyler's Z. Physiol. Chem., 349: 429–432, 1968.
23.
SanderJ., SchweinsbergF.: In vivo and in vitro experiments on the formation of N-nitroso compounds from amines or amides and nitrates or nitrites. IARC Scientific Publications No.3: 97–103, 1971.
24.
SenN.P., SmithD.C., SchwinghamerL.: Formation of N-nitrosamines from secondary amines and nitrite in human and animal gastric juice. Food Cosmet. Toxicol., 7: 301–307, 1969.
25.
TannenbaumS.R., SinskayA.J., WeismanM., BishopW.: Nitrite in human saliva. Its possible relatioship to nitrosamine formation. J. Natl. Cancer Inst., 53: 79–84, 1974.
26.
TredtH.J., MullerK.H., ThieleH.J., LestinH., BrashC., SeifertA., StotkoM.: Ergebnisse eines Nierenscreenings bei 16000 Presonen eines Landkreises 11 Klinische Resultate. Dtsch. Ges. Wesen., 25: 1323–1327, 1970.
