Mobilization of granulocytes into a serum-filled chamber, histochemical nitroblue tetrazolium (NBT) reduction tests, and phagocytosis were performed in 11 patients with solid tumors treated with surgical excision and chemotherapy and in 22 untreated or surgically treated patients. The results revealed a decreased mobilization (p < 0.001) and an impaired capability of neutrophils to reduce NBT after stimulation (p < 0.05) in both groups of patients. The decrease in the values in the stimulated reduction of NBT was more pronounced in untreated patients than in treated ones. At the same time the phagocytic activity of neutrophils on Candida albicans, which was decreased (p < 0.01) in untreated patients, was normal in those who had been treated with chemotherapy. There were no distinctive correlations between circulating immune complexes and granulocyte function. We propose that this newly demostrated defect in neutrophil mobilization and low median C. albicans-stimulated NBT reduction contributes more in the evolution of the tumor than in the pathogenesis of infections and that chemotherapy seems to restore a better granulocyte function.
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References
1.
BergJ.W.: Inflammation and prognosis in breast Cancer: A search for host resistance.Cancer Res., 12: 714–720, 1959.
2.
DeinardA.FortunyI.TheologidesA.KennedyB.: Studies on the neutropenia of cancer chemotherapy.Cancer, 33: 1210–1218, 1974.
3.
DvorakP.A.ConnellA.B.ProppeK.DvorakH.F.: Immunologic rejection of mammary adenocarcinoma (TA3-St) in C57BL/6 mice: Participation of neutrophils and activated macrophages with fibrin formation.I. Immunol., 120: 1240–1248, 1978.
4.
EvansR.AlexanderP.: Cooperation of immune lymphoid cells with macrophages in tumour immunity.Nature (Lond.), 228: 620–622, 1970.
5.
GowlandE.: Studies on the emigration of polymorphonuclear leucocytes from skin lesions in man.J. Pathol. Bacteriol., 87: 347–352, 1964.
6.
HagemeisterF.B.Jr., BujdarA.U.BlumenscheinG.R.: Causes of death in breast.Cancer, 46: 162–167, 1980.
7.
HaimN.ObedeanuN.MeshulamT.RobinsonE.MerzbachD.: Spontaneous and stimulated nitroblue tetrazolium tests of leukocytes from patients with solid malignant tumors.Am. J. Clin. Pathol., 68: 570–574, 1977.
8.
HaimH.ObedeanuN.MeshulamT.RobinsonE.SchreiberH.MerzbachD.: Neutrophil function in cured cancer patients.Cancer, 40: 2273–2277, 1977.
9.
HancockB.W.BruceL.RichmondJ.: Neutrophil function in lymphoreticular malignancy.Br. J. Cancer, 33: 496–500, 1976.
10.
HughesL.E.MeckayW.D.: Suppression of the tuberculin response in malignant disease.Br. Med. J., 2: 1346–1348, 1965.
11.
InagakiJ.RodriquezV.BodeyG.P.: Causes of death in cancer patients.Cancer, 33: 568–573, 1974.
12.
LehrerR.I.ClineM.J.: Interaction of Candida albicans with human leukocytes and serum.J. Bacteriol., 98: 996–1004, 1969.
13.
LehrerR.I.ClineM.J.: Leukocyte candidacidal activity and resistance to systemic candidiasis in patients with cancer.Cancer, 27: 1211–1217, 1971.
14.
LevinJ.TomasuloP.A.OserR.S.: Detection of endotoxin in human blood and demonstration of an inhibitor.J. Lab. Clin. Med., 75: 903–911, 1970.
15.
MaderazoE.C.AntonT.F.WardP.A.: Serum-associated inhibition of leukotaxis in humans with cancer.Clin. Immunol. Immunopathol., 9: 166–176, 1978.
16.
MancaF.MiglioriniP.BombardiertS.CeladaF.: An enzymatically active antigen-antibody probe to measure circulating immune complexes by competition. Use of the E. coli beta-galactosidase in the probe and of bovine conglutinin as the complement-binding reagent.Clin. Immunol. Immunopathol., 16: 131–141, 1980.
17.
MatulaG.PetersonP.Y.: Spontaneous in vitro reduction of nitroblue tetrazolium by neutrophils of adult patients with bacterial infections.N. Engl. J. Med., 285: 311–317, 1971.
18.
McCoyJ.L.JeromeE.F.DeanJ.H.CannonG.B.AlfordT.C.DoeringT.HebermanR.B.: Inhibition of leukocytes migration by tumor-associated antigens in soluble extracts of human breast carcinoma.J. Natl. Cancer Inst., 53: 11–17, 1974.
19.
McRipleyR.J.SelvarajR.J.GlovskyM.M.SbarraA.J.: The role of the phagocyte in host-parasite interactions. V. Phagocytic and bactericidal activities of leukocytes from patients with different neoplastic disorders.Cancer Res., 27: 674–685, 1967.
20.
MilesA.A.MilesE.M.BurkeJ.: The value and duration of defence reactions of the skin to the primary lodgement of bacteria.Br. J. Exp. Pathol., 38: 79–96, 1957.
21.
PapsideroL.D.NemotoT.SnydermanM.C.ChuT.M.: Immune complexes in breast cancer patients as detected by CIq binding.Cancer, 44: 1636–1640, 1979.
22.
ParkH.B.FikrigS.M.SmithwickE.M.: Infection and nitroblue-tetrazolium reduction by neutrophils. A diagnostic aid.Lancet, ii: 532–534, 1968.
23.
PhairJ.P.RiesingK.J.MetzgerE.: Bacteremic infection and malnutrition in patients with solid tumors.Cancer, 45: 2702–2706, 1980.
24.
PickeringL.K.AndersonD.C.ChoiS.FeiginR.D.: Leukocyte function in children with malignancies.Cancer, 35: 1365–1371, 1975.
25.
PreisigE.HitzigW.H.: Nitroblue-tetrazolium test for the detection of chronic granulomatous disease.Technical modification. Eur. J. Clin. Invest., 1: 409–412, 1971.
26.
RobertsM.M.Jones-WilliamsW.: The delayed hypersensitivity reaction in breast cancer.Br. J. Surg., 61: 549–552, 1974.
27.
SennH.HollandJ.BanerjeeT.: Kinetic and comparative studies on localized leukocyte mobilization in normal man.J. Lab. Clin. Med., 74: 742–756, 1969.
28.
SherwinR.P.RichtersA.: Pathobiology of lymphocyte interactions. In Pathology Annuals. 1973.SommersS.C. editor, pp. 379–406. Appleton-Century-Crofts Inc., New York1973.
29.
SutherlandR.M.InchW.R.McCredieJ.A.: Phytohemagglutinin (PHA)-induced transformation of lymphocytes from patients with cancer.Cancer, 75: 574–578, 1971.
30.
TanakaF.YonemotoR.H.WaldmanS.R.: Blocking factors in sera of breast cancer patients.Cancer, 43: 838–847, 1979.
31.
TheologidesA.: Pathogenesis of cachexia in cancer.Cancer, 29: 484–488, 1972.
32.
TwomeyP.L.CatelonaW.J.CretienP.B.: Cellular immunity in cured cancer patients.Cancer, 33: 568–573, 1974.
33.
WaneboH.J.ThalerH.T.HansenJ.A.RosenP.P.RobbinsG.F.UrbanJ.A.OettgenH.F.GoodR.A.: Immunologic reactivity in patients with primary operable breast cancer.Cancer, 41: 84–94, 1978.
34.
WardP.A.CohenS.: Regulation of inflammatory responses in neoplastic disease.Mechanisms of tumor immunity.GreenI.CohenS.Mc CluskeyR.T. (eds.). pp. 305–313. John Wiley and Sons, New York1977.
35.
WhittakerM.E.ReesK.ClarkG.C.: Reduced lymphocyte trasformation in breast cancer.Lancet,i: 892–893, 1971.
