Peripheral blood lymphocytes from bladder cancer patients display in vitro cytotoxicity against established bladder tumor target cells. Following surgery and after separation of lymphocytes in non-T and T with high affinity and low affinity for sheep erythrocytes fractions, the cytotoxic activity was significantly decreased in all subsets. After BCG therapy the cytotoxic capacity was restored and the effect was equally distributed in the different subpopulations. The monocyte-enriched fraction did not show any change in the cytotoxic activity after treatment.
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References
1.
AntonaciS., PiccininnoA., DammaccoF., BonomoL.: Natural cytotoxicity: Effector cells and role of the immunoglobulins. Haematologica, 66: 25–31, 1981.
BastR.C., ZbarB., BorsosT., RappH.J.: BCG and cancer. N. Engl. J. Med., 290: 1458–1469, 1974.
4.
BloomM., OssorioR.C., BrosmanS.A.: Cell-mediated cytotoxicity against human bladder cancer. Int. J. Cancer, 14: 326–334, 1974.
5.
BubenikJ., PerlmannP., HelmsteinK., MobergerG.: Cellular and humoral immune responses to human urinary bladder carcinoma. Int. J. Cancer, 5: 310–319, 1970.
6.
BubenikJ., BaresovaM., ViklickyV., JakoubkovaJ., SairenovaH., DonnerJ.: Established cell line of urinary bladder carcinoma (T24) containing tumor-specific antigen. Int. J. Cancer, 11: 765–773, 1973.
7.
FoghJ.: Human tumor cells in vitro, FoughJ. Ed., Plenum Press, New York, 115–159, 1975.
8.
GeffardM., Orbach-ArbouysS.: Enhancement of T suppressor activity in mice by high doses of BCG. Cancer Immunol. Imunother., 1: 41–46, 1976.
9.
HeppnerG., HenryE., StolbachL., CummingsF., McDonoughE., CalabresiP.: Problems in the clinical use of the microcytotoxicity assay for measuring cell-mediated immunity to tumor cells. Cancer Res., 35: 1931–1937, 1975.
10.
KallM.A., KorenH.S.: Heterogeneity of human natural killer cell populations. Cell. Immunol., 40: 58–68, 1978.
11.
KiuchiU., TakasugiM.: The nonselective cytotoxic cell (N cell). J. Natl. Cancer Inst., 56: 575–582, 1976.
12.
MeltzerM.S., TuckerR.W., SynfordK.K., LeonardE.J.: Interaction of BCG-activated macrophages with neoplastic and non-neoplastic cell lines in vitro: quantitation of the cytotoxic reaction by release of tritiated thymidine from prelabelled target cells. J. Natl. Cancer Inst., 54: 1177–1181, 1975.
13.
MoralesA., EidingerD., BruceW.: Intracavitary Bacillus Calmette-Guerin in the treatment of superficial bladder tumors. J. Urol., 116: 180–183, 1976.
14.
O'TooleC., PerlmannP., UnsgaardB., AlmgardL.E., JohanssonB., MobergerG., EdsmyrF.: Cellular immunity to human urinary bladder carcinoma. II. Effect of surgery and preoperative irradiation. Int. J. Cancer, 10: 92–98, 1972.
15.
PapeG.R., TroyeM., PerlmannP.: Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. II. Cytotoxicity to allogeneic and autochthonous tumor cells in tissue culture. J. Immunol., 118: 1925–1930, 1977.
16.
PerlmannH., PerlmannP., PapeG.R., HalldenG.: Purification, fractionation and assay of antibody-dependent lymphocytic effector cells (K cells) in human blood. Scand. J. Immunol., 5: suppl. 5, 57–68, 1976.
17.
SimonH.B., SheagrenJ.N.: Cellular immunity in vitro. I. Immunologically mediated enhancement of macrophage bactericidal capacity. J. Exp. Med., 133: 1377–1389, 1971.
18.
TakasugiM., AkiraD., TakasugiJ., MickeyM.R.: Specificities in human cell-mediated cytotoxicity. J. Natl. Cancer Inst., 59: 69–75, 1977.
19.
ThatcherN., CrowtherD.: Changes in nonspecific lymphoid (NK, K, T Cell) cytotoxicity following BCG immunisation of healthy subjects. Cancer Immunol. Immunother., 5: 105–107, 1978.
20.
TroyeM., PapeG.R., PaulieS., KarlssonM., PerlmannP., BlomgrenH., JohanssonB.: Disease-related in vitro cytotoxicity in patients with transitional cell carcinoma of the urinary bladder. Comparison with other malignancies, acute cystitis and healthy donors. Cancer Immunol. Immunother., 8: 13–26, 1980.
21.
Union internationale contre le cancer. TNM. Classification of malignant tumors.UICC, Geneva, 1974.
22.
WestW.H., CannonG.B., KayH.D., BonnardG. D., HerbermanR.B.: Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol., 118: 355–361, 1977.
23.
WestW.H., PayneS.M., WeeseJ.L., HerbermanR.B.: Human T lymphocyte subpopulations: Correlation between E-rosette forming affinity and expression of the Fc receptor. J. Immunol., 119: 548–554, 1977.
24.
WolfeS., TraceyD.E., HenneyC.S.: Induction of natural killer cells by BCG. Nature, 262: 584–586, 1976.
