Histones prepared from human lung and human lung carcinoma were analyzed by a 2-dimensional elecvtrophoresis method able to separate histone subfractions. The comparative analysis of electrophoretograms showed differences in the relative amounts of H1, H2A, and H3 histone subfractions extracted from human lung and human lung carcinoma chromatin.
Get full access to this article
View all access options for this article.
References
1.
BlanksteinL.A., LevyS.B.: Changes in histone f2 a2 associated with proliferation of Friend leukaemic cells. Nature, 260: 638–640, 1976.
2.
BorunT.W., GabrielliF., AjiroK., ZweidlerA., BaglioniC.: Further evidence of transcriptional and translational control of histone messenger RNA during the HeLa S3 cycle. Cell, 4: 59–67, 1975.
3.
FranklingS.G., ZweidlerA.: Non-allelic variants of histones 2a, 2b and 3 in mammals. Nature, 266: 273–275, 1977.
4.
GabrielliF., BaglioniC.: Maternal messenger RNA and histone synthesis in embryos of the surf clam Spisula Solidissima. Dev. Biol., 43: 254–263, 1975.
5.
GarrardW.T., HancockR.: Preparation of chromatin from animal tissues and cultured cell. Methods Cell Biol., 17: 27–48, 1978.
JohnsE.W., GodwinG.H., WalkerJ.M., SandersC.: Chromosomal proteins related to histones. In: Structure and Function of Chromatin. Ciba Foundation Symposium28(new Series), pp. 95–112, Amsterdam Elsevier Excepta Medica, North Holland, 1975.
8.
LaemmliU.K.: Cleavage of structural protein during assembly of the head of bacteriophage T4. Nature, 227: 680–681, 1970.
9.
MarksD.B., KanefskyT., KellerB.J., MarksA.D.: The presence of histone H1° in human tissues. Cancer Res., 35: 886–889, 1975.
10.
NewrockK.M., AlfagameC.R., NardiR.V., CohenL.H.: Histone changes during chromatin remodeling in embryogenesis. Cold Spring Harbor Symp. Quant. Biol, 42: 421–431, 1978.
RudermanJ., GrossP.R.: Histones and histone synthesis in sea urchin development. Dev. Biol., 36: 286–318, 1974.
13.
SeidmanM.M., LevineA.J., WeintraubH.: The asymmetric segregation of parental nucleosomes during chromosome replication. Cell, 18: 439, 1979.
14.
Unger-UllmanC., ModakS.P.: Gel electrophoretic analysis of histones in lens epthelium, lens fiber, liver, brain, and erythrocytes of late chick embryos. Differentiation, 12: 135–144, 1979.
15.
ZweidlerA.: Resolution of histones by polyacrylamide gel electrophoresis in presence of non ionic detergents. Methods Cell. Biol., 17: 223–232, 1978.