Intercellular glycosaminoglycans (GAGs) from various tissues were analyzed by cellulose acetate electrophoresis and enzymatic treatment with specific mucopolysaccharidases. Each tissue exhibits a particular composition of sulfated and unsulfated molecular species. Invariably, malignant human neoplasias and their metastases show striking variations in the electrophoretic pattern typical of the corresponding normal tissue. An absolute or relative increase in surface ChS A/C and HA seems to be a consistent feature of neoplastic transformation. On the other hand, the GAGs composition of benign noninfiltrative tumors does not vary greatly with respect to the original normal tissue.
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References
1.
AtherlyA.G., BarnhardB.J., KraemerP.: Growth and biochemical characteristics of a detachment variant of CHO cells.J. Cell. Physiol.,89: 375–386, 1977.
2.
Augusti ToccoG., ChiarugiV.P.: Surface glycosaminoglycans as a differentiation cofactor in neuroblastoma cell culture.Cell Differ.,5: 161–170, 1976.
3.
CappellettiR., Del RossoM., ChiarugiV.P.: Rapid mutisample separation of the five most widespread animal glycosaminoglycans.Anal. Biochem.,93: 37–40, 1979.
4.
CappellettiR., Del RossoM., ChiarugiV.P.: A new electrophoretic method for the separation of all known animal glycosaminoglycans in a single run.Anal. Biochem, in press, 1979.
5.
ChiarugiV.P.: Cell coat glycosaminoglycans in cellular transformation and differentiation.Exp. Cell Biol.,44: 251–259, 1976.
6.
ChiarugiV.P., VannucchiS.: Surface heparan sulphate as a control element in eukariotic cells: A working model.J. Theor. Biol.,61: 459–475, 1976.
7.
ChiarugiV.P., VannucchiS., Del RossoM., UrbanoP.: Surface polyanions in normal and virally transformed cells. Proc. 11th Int.Cancer Congress,1: 138–141, 1974.
8.
ChiarugiV.P., VannucchiS., UrbanoP.: Exposure of trypsin removable sulphated polyanions on the surface of normal and virally transformed BHK 21/C13 cells.Biochim. Biophys. Acta,345: 283–293, 1974.
9.
ChiarugiV.P., VannucchiS., CellaM.C., FibbiG., Del RossoM., CappellettiR.: Intercellular glycosaminoglycans in normal and neoplastic tissues.Cancer Res.,38: 4717–4723, 1978.
10.
DietrichC.P., SampaioL.O., ToledoO.M.S.: Characteristic distribution of sulfated mucopolysaccharides in different tissues and in their respective mitochondria.Biochim. Biophys. Res. Commun.,71: 1–10, 1976.
11.
DietrichC.P., SampaioL.O., ToledoO.M.S., CassaroC.M.E.: Cell recognition and adhesiveness: A possible biological role for the sulfated mucopolysaccharides.Biochim. Biophys. Res. Commun.,75: 329–336, 1977.
12.
DietrichC.P., DietrichS.M.C.: Simple micromethod for identification of heparin and other acidic mucopolysaccharides from mammalian tissues.Anal. Biochem.,46: 209–215, 1972.
13.
DietrichC.P., SilvaM.E., MichelacciY.M.: Sequential degradation of heparin in Flavobacterium heparinum. J.Biol. Chem.,248: 6408–6415, 1973.
14.
FranssonL.A., CosterL.: Interaction between dermatosulfate chains structural studies on aggregating glycan chains and oligosaccharides with affinity for dermatolsulfate substituted agarose.Biochim. Biophys. Acta,582: 132–144, 1979.
15.
LudowiegJ., VenneslandB., DorfmanA.: The mechanism of action of hyaluronidase.J. Biol. Chem.,236: 333–340, 1961.
16.
MeyerK.: Biochemistry and biology of mucopolysaccharides.Am. J. Med.,47: 664–671, 1969.
17.
MichelacciY.M., DietrichC.P.: Structure of chondroitin sulfates. Analyses of the products formed from chondroitin sulfates A and C by the action of the chondroitinases C and AC from Flavobacterium heparinum. Biochim.Biophys. Acta,451: 436–443, 1976.
18.
TakeuchiJ.: Effect of chondroitin sulfate on the growth of solid Ehrlich ascites tumor under the influences of other interstitial components.Cancer Res.,28: 1520–1523, 1968.
19.
UnderhillC.B., KellerJ.M.: Heparan sulfates of mouse cells. Analysis of parent and transformed 3T3 cell lines.J. Cell. Physiol.,90: 53–60, 1977.
20.
VannucchiS., ChiarugiV.P.: Surface exposure of glycosaminoglycans in resting, growing and transformed 3T3 cells.J. Cell. Physiol.,90: 503–510, 1977.
