Two tumor cell lines adapted to grow in vitro were originated from an explant of lung metastases of Lewis lung carcinoma. These lines differ in their malignancy when reinoculated into syngeneic animals; nevertheless, they do not show any difference for their in vitro clonogenic ability. From these lines 2 in vivo sublines of 3LL carcinoma were developed. The TD 50 of the 2 in vivo sublines are different, and both the values obtained are lower than that of the original line. These results are interpreted as a selection of more malignant tumor cell lines.
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References
1.
CourtenayV.D.: A soft agar colony assay for Lewis lung tumour and B16 melanoma taken directly from the mouse.Br. J. Cancer,34: 39–45, 1976.
2.
DethlefsenL.A., PrewittJ.M.S., MendelsohnM.L.: Analysis of tumour growth curve.J. Nat. Cancer Inst.,40: 389–405, 1968.
3.
De WysW.D.: A quantitative model for the study of the growth and treatment of a tumor and its metastases with correlation between proliferative state and sensitivity to cyclophosphamide.Cancer Res.,32: 367–373, 1972.
4.
FidlerI.J.: Tumor heterogeneity and the biology of cancer invasive and metastasis.Cancer Res.,38: 2651–2660, 1978.
5.
FinneyD.J.: Statistical Method in Biological Assay. pp. 525–529, 2nd ed.Griffin and Co., London, 1964.
6.
GrecoC., CorsiA., CaputoM., CavallariA., CalabresiF.: Cyclophosphamide and iphosphamide against Lewis lung carcinoma: Evaluation of toxic and therapeutic effects.Tumori,65: 169–180, 1979.
7.
OvejeraA.A., JohnsonR.K., GoldinA.: Growth characteristics and chemotherapeutic response of intravenously implanted Lewis lung carcinoma.Cancer Chem. Rep.,5: 111–125, 1975.
8.
PuckT.T., MarcusP.I., CieciuraS.J.: Clonal growth of mammalian cells « in vitro ».J. Exp. Med.,103: 273–284, 1956.
9.
PuckT.T., MarcusP.I.: A rapid method of viable cell tritation and clone production with HeLa cells in tissue culture: The use of X-irradiated cells to supply condition factors.Proc. Nat. Acad. Sci.,41: 432–437, 1955.
10.
RockwellS.: «In vivo »-« in vitro» tumor systems: New models for studying the response of tumors to therapy.Lab. Anim. Sci.,27: 831–851, 1977.
11.
RossoR., DonelliM.G., GarattiniS.: Studies on cancer dissemination.Cancer Res.,27: 1225–1231, 1967.
12.
SteelG.G., AdamsK.: Stem-cell survival and tumor control in the Lewis lung carcinoma.Cancer Res.,35: 1530–1535, 1975.
13.
SugiuraK., StockC.C.: Studies in a tumor spectrum III: The effects of phosphoramides on the growth of a variety of mouse and rat tumors.Cancer Res.,15: 38–51, 1955.
