Alien Histocompatibility Determinants on the Cell Surface of Sarcomas Induced by Methylcholanthrene. II. in Vitro Serological Studies 1

Abstract

Italian

In vitro serological studies were carried out in an attempt to identify the foreign histocompatibility antigens previously detected by transplantation methods on the cell surface of 2 methylcholanthrene-induced BALB/c fibrosarcomas (TZ15 and ST2). The reactivity of the anti-TZ15 and anti-ST2 syngeneic sera and of the C57BL/6J anti-TZ15 and anti-ST2 sera (the latters preabsorbed on normal BALB/c lymphoid cells) was evaluated on normal lymph node cells of C3Hf, C57BL/6J, DBA/2 and AKR strains by the complement-dependent cytotoxicity and on normal fibroblasts of C3Hf and C57BL/6J mice by the isotopic antiglobulin assay. The ability of in vitro-plated TZ15 and ST2 cells to bind BALB/c anti-C3Hf, anti-C57BL/6J, anti-AKR and anti-DBA/2 alloantisera was also examined by the antiglobulin assay. Contrary to the in vivo data, no cross-reactions were found between TZ15 and AKR normal cells, since both syngeneic and allogeneic anti-TZ15 sera were not cytotoxic to C3Hf, C57BL/6J or AKR lymphoid cells nor could BALB/c anti-C3Hf, anti-C57BL/6J or anti-AKR sera bind to TZ15 tumor cells in the antiglobulin assay. Both BALB/c anti-ST2 and C57BL/6J anti-ST2 sera were also ineffective in killing normal C3Hf, C57BL/6J, AKR and DBA/2 lymphoid cells in the complement–dependent cytotoxic assay, whereas a weak but significant binding of the syngeneic anti-ST2 serum to C57BL/6J but not to C3Hf fibroblasts was detected by the antiglobulin test. ST2 but not TZ15 cells were also able to significantly absorb the complement–dependent cytotoxic activity of a BALB/c anti-C57BL/6J but not of a BALB/c anti-C3Hf antiserum. In addition, BALB/c anti-C3Hf, anti-C57BL/6J and anti-AKR sera but not BALB/c anti-DBA/2 serum strongly bound to ST2 cells in the antiglobulin test; this binding activity was absorbed by C3Hf, C57BL/6J, AKR, and NIH but not BALB/c and DBA/2 lymphoid cells. Thus, among the cross-reactions observed in vivo between ST2 and normal cells of C3Hf, DBA/2 and C57BL/6J strains, only C57BL/6J normal antigens were detectable by serology. A new but still undefined system of alien normal antigens shared by C3Hf, AKR, C57BL/6J, NIH lymphoid and ST2 cells was revealed by the BALB/c alloantisera in the antiglobulin assay. The relationship of these findings with those previously obtained in the transplantation study with the same tumors is discussed.

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