The influence of autologous serum on DNA synthesis and phytohemagglutinin (PHA) or pokeweed mitogen (PWM) stimulation of peripheral blood lymphocytes from mammary tumor-bearing women has been studied. Blood samples were collected from patients at the 4 stages of the TNM classification who had not undergone any therapeutic treatment. Lymphocyte functions were significantly reduced only in patients with the largest tumors and in the latest stage of disease. Autologous cancer sera inhibited spontaneous DNA synthesis of lymphocytes and did not inhibit lymphocyte stimulation by mitogens. It is possible that unrecognized inhibiting and stimulating factors, possibly acting in synergism with mitogens, are present in mammary cancer sera.
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References
1.
AlfordC., HollinsheadA. C., HerbermanR. B.: Delayed cutaneous hypersensitivity reactions to extracts of malignant and normal human breast cells. Ann. Surg., 178: 20–27, 1973.
2.
BoyumA.: Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest., 21(Suppl. 97): 1–109, 1968.
3.
CurrieG. A., BashamC.: Serum mediated inhibition of immunological reactions of the patient to his own tumor: a possible role for circulating antigen. Br. J. Cancer, 26: 427–438, 1972.
4.
EilbertF., MortonD. L.: Sarcoma specific antigens: detection by complement fixation with serum from sarcoma patients. J. Nat. Cancer Inst., 44: 651–656, 1970.
5.
FossatiG., CanevariS., Della PortaG., BalzariniG. P., VeronesiU.: Cellular immunity to human breast carcinoma. Int. J. Cancer, 10: 391–396, 1972.
GoldP., FreedmanS. O.: Specific carcinoembryonic antigens of the human digestive system. J. Exp. Med., 122: 467–481, 1965.
8.
HartzmanR. J., SegallM., BachM. L., BachF. H.: Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation, 11: 268–273, 1971.
9.
HellströmI., HellströmK. E., SjögrenH. O., WarnerG. A.: Demonstration of cell-mediated immunity to human neoplasms of various histological types. Int. J. Cancer, 7: 1–16, 1971.
10.
HellströmI., SjögrenH. O., WarnerG. A., HellströmK. E.: Blocking of cell–mediated tumor immunity by sera from patients with growing neoplasms. Int. J. Cancer, 7: 226–237, 1971.
JehnU. W., NathansonL., SchwartzR. S., SkinnerM.: In vitro lymphocyte stimulation by a soluble antigen from malignant melanoma. New Eng. J. Med., 283: 329–333, 1970.
13.
LeviM.: Antigenicity of ovarian and cervical malignancies with a view towards possible immunodiagnosis. Am. J. Obstet. Gynec., 109: 689–698, 1971.
14.
MatchettK. M., HuangA. T., KremerW. B.: Impaired lymphocyte transformation in Hodgkin's disease evidence for depletion of circulating T-lymphocytes. J. Clin. Invest., 52: 1908–1917, 1973.
15.
Mc CoyJ. L., JeromeL. P., DeanJ. H., CannonG. B., AlfordT. C., DoeringT., HerbermanR. B.: Inhibition of leukocyte migration by tumor-associated antigens in soluble extracts of human breast carcinoma. J. Nat. Cancer Inst., 53: 11–17, 1974.
16.
NimbergR. B., GlasgowA. H., MenzoianJ. O., ConstantianM. B., CooperbandS. R., MannickJ. A., SchmidK.: Isolation of an immunosuppressive peptide fraction from the serum of cancer patients. Cancer Res., 35: 1489–1494, 1975.
17.
RiecheK., ArndtA., PasternakG.: Cellular immunity in mammary cancer patients as measured by the leukocyte migration test (LMT). A follow-up study. Int. J. Cancer, 17: 212–218, 1976.
18.
SegallA., WeilerO., GeninJ., LacourJ., LacourF.: In vitro study of cellular immunity against autochthonous human cancer. Int. J. Cancer, 9: 417–425, 1972.
19.
SilkM.: Effect of plasma from patients with carcinoma of in vitro lymphocyte transformation. Cancer, 20: 2088–2089, 1967.
20.
VankyF., KleinE., StjernswärdJ., TrempeG.: Lymphocyte stimulation by autologous tumor cells in the presence of serum from the same patient or from healthy donors. Int. J. Cancer, 16: 850–860, 1975.
21.
WhiteheadR. H., ThatcherJ., TeasdaleC., RobertsG. P., HughesL. E.: T and B lymphocytes in breast cancer stage relationship and abrogation of T-lymphocyte depression by enzyme treatment in vitro. Lancet, 1: 330–333, 1976.
22.
WhittakerM. G., RefsK., ClarkC. G.: Reduced lymphocyte transformation in breast cancer. Lancet, 1: 892–893, 1971.
