In a series of experiments the prolonged feeding of rats with thioacetamide and 4-dimethylaminoazobenzene provoked an increase in mitochondrial calcium. It seems likely that this continuous mitochondrial overloading with Ca2+ is related to metabolic and functional changes of the liver cell leading to a neoplastic transformation. The drastic changes in cell membrane permeability of cholangiocarcinoma tumor cells, reflected by an increased concentration of extracellular cations (calcium and sodium), while the intracellular cations tended to decrease, indicate that the mitochondrial function of specification of cell membrane characteristics is possibly impaired by that calcification.
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