Incidence,Growth and Antigenicity of Fibrosarcomas Induced by Teflon Disc in Mice 1

Abstract

Italian

Subcutaneous sarcomas were induced in BALB/c, C3Hf Dp, and C57BL/He female mice by implantation of a Teflon disc or by injection of 7,12-dimethylbenz(a)anthracene (DMBA). The incidence of DMBA tumors was homogeneously high (60–86 %) in the 3 strains, and the latency varied from 16 to 26 weeks from treatment. Teflon-induced tumors developed, in the BALB/c, C3Hf/Dp, C57BL/He mice, in 44, 94, and 30 %, with a mean latency of 78, 61, and 82 weeks respectively. Evaluation of the growing capacity of 4 DMBA-induced and 14 Teflon-induced fibrosarcomas at the first and second transplant passage showed that Teflon tumors grew faster and needed a smaller cell dose to take than the DMBA tumors. Transplantation type antigens were detected only on the chemically induced tumors. Cross-reacting antigens were detected on 3 of the 5 Teflon-induced tumors by an in vitro assay for cell-mediated cytotoxicity.

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References

Bischoff

Bryson

: Carcinogenesis through solid state surface. Progr. Exp. Tumor Res., 5: 85–133, 1964.

Carbone

Parmiani

: Nonimmunogenic sarcomas induced by 3-methylcholanthrene treatment of murine fibroblasts in diffusion chambers. J. Nat. Cancer Inst, 55: 1195–1197, 1975.

Hellström

K. E.

: Cell-bound immunity to autologous and syngeneic mouse tumors-induced by methylcholanthrene and plastic discs. Science, 156: 981–983, 1967.

Hellström

K. E.

Pierce

G. E.

: In vitro studies of immune reactions against autochthonous and syngeneic mouse tumors induced by methylcholanthrene and plastic discs. Int. J. Cancer, 3: 467–482, 1968.

Horn

K. H.

Pasternak

Graffi

: Experiments on the induction of tumor-specific resistance against isologous transplants of sarcomas induced by implantation of plastic pellets. Arch. Immunol. Ther. Exp., 14: 737–742, 1966.

Johnson

K. H.

Buoen

L. C.

Brand

: Polymer tumorigenesis: Clonal determination of histopathological characteristics during early preneoplasia; relationships to karyotype, mouse strain, and sex. J. Nat. Cancer Inst, 44: 785–793, 1970.

Klein

Sjorgen

H. O.

Klein

: Demonstration of host resistance against sarcomas induced by implantation of cellophane films in isologous (syngeneic) recipients. Cancer Res., 23: 84–92, 1963.

Menard

Colnaghi

M. I.

Della Porta

: Embryonic antigens and growth of murine fibrosarcomata. Br. J. Cancer, 30: 524–531, 1974.

Menard

Colnaghi

M. I.

Della Porta

: In vitro demonstration of tumor-specific common antigens and embryonal antigens in murine fibrosarcomas induced by 7,12-dimethylbenz(a)anthracene. Cancer Res., 33: 478–481, 1973.

10.

Prehn

R. T.

: The role of immune mechanisms in the biology of chemically and physically induced tumors. pp. 475–485. In: Conceptual Advances in Immunology and Oncology. 16th Annual Symp. Fundamental Cancer Research, 1962.

11.

Sanford

B. H.

Soo

S. F.

: Fibrosarcoma induction in immunodepressed mice. J. Immunol, 110: 595, 1973.

12.

Shimkin

M. B.

Bryan

W. R.

: Morphology and growth of subcutaneous tumors induced with carcinogenic hydrocarbons in strain C3H male mice. J. Nat. Cancer Inst, 4: 25–35, 1943.

13.

Tomatis

: Subcutaneous carcinogenesis by implants and by 7,12-dimethylbenz(a) anthracene. Tumori, 52: 1–16, 1966.

14.

Tomatis

Parmi

: Effect of perinatal administration of 7,12-dimethylbenz(a)-anthracene on the later response to a subcutaneous teflon implant. Tumori, 57: 55–62, 1971.

15.

Vasiliev

J. M.

Olshevskaja

L. V.

Raikhlin

N. T.

Ivanova

O. J.

: Comparative study of alterations induced by 7,12-dimethylbenz(a)anthracene and polymer films in the subcutaneous connective tissue of rats. J. Nat. Cancer Inst, 28: 515–559, 1962.