The effect of Hodgkin patient cytotoxic sera on the electrophoretic mobility of normal and Hodgkin peripheral blood allo-lymphocytes has been studied. Contact with cytotoxic serum determined a significant decrease in the electrophoretic mobility of lymphocytes, due to the presence of cytotoxic antibody on the lymphocyte surface. The antibody seems to be directed against T-lymphocytes. The results are discussed in the light of the preceding data by the authors on the role of anti-T-autoantibodies in Hodgkin's disease.
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References
1.
AnderssonL. C., NordlingS., HayryP.: Fractionation of mouse T and B lymphocytes by preparative cell electrophoresis.Cell. Immun., 8: 235–248, 1973.
2.
BertG., Laiolo di CossanoD., PeccoP.: The detection by cellular electrophoresis of surface antibodies in human lymphocytes.Clin. Exp. Immun., 5: 669–672, 1969.
3.
Del GiaccoG. S., ManconiP. E., TognellaS., MantovaniG., GrifoniV.: IT-linfociti come cellula bersaglio degli anticorpi antilinfocitari nella malattia di Hodgkin.Com. XXIV Congr. Soc. Ital. Emat., Pavia, 1973.
4.
Del GiaccoG. S., ManconiP. E., TognellaS., MantovaniG., GrifoniV.: La situazione immunologica della malattia di Hodgkin.Relaz. XIV Congr. Soc. Jonico-Salentina Med. Chir., Lecce, 1974.
5.
Del GiaccoG. S., ManconiP. E., TagnellaS., MantovaniG., TurnoR., GrifoniV.: Anticorpi antilinfocitari e deficit immunitario cellulare nella malattia di Hodgkin.Com. XXIV Congr. Soc. Ital. Emat., Pavia, 1973.
6.
GrifoniV.: Recent immunological findings in Hodgkin's disease.In: Symposium: Immunological aspects of Hodgkin's disease. Tumori, 59: 363–374, 1973.
7.
GrifoniV.: Anticorpi antilinfociti nella malattia di Hodgkin. In: I linfomi maligni.BucalossiP., VeronesiU., BonadonnaG., EmaneulliH. (eds.), pp. 313–318. CEA, Mlano, 1974.
8.
GrifoniV., Del GiaccoG. S., ManconiP. E., TognellaS.: Surface immunoglobulins on lymphocytes in Hodgkin's disease.LancetI, 848–848, 1972.
9.
GrifoniV., Del GiaccoG. S., TognellaS., ManconiP. E., MantovaniG.: Lymphocytotoxins in Hodgkin's disease.Ital. I. Immun. Immunopath., 1: 21–31, 1970.
10.
GrifoniV., Del GiaccoG. S., TognellaS., MantovaniG., ManconiP. E.: Aspetti immunologia della malattia di Hodgkin.II Congr. Soc. Ital. Immun. Immunopat., Roma, 1973.
11.
GrifoniV., TognellaS., Del GiaccoG. S., MantovaniG., ManconiP.E., FlorisC., CengiarottiL.: Effects of hodgkinian cytotoxic serum on lymphocytes cultured in vitro.Meeting Europ. Tissue Cult. Soc., Genoa, 1974.
12.
GrifoniV., TognellaS., MantovaniG., Del GiaccoG. S.: Studi di citoferometria nella malattia di Hodgkin.Haematologica, 57: 533–535, 1972.
13.
SabolovicD., SabolovicN., DumontF.: Identification of T and B cells in mouse and man.LancetII, 927–927, 1972.
14.
SteinG., FladH. D., PabstR., TrepelF.: Separation of human lymphocytes by free-flow electrophoresis.Biomedicine (Express), 19: 388–391, 1973.
15.
TerasakiP. I., McClellandJ. D.: Microdroplet cytotoxicity test. In: Manual of tissue typing techniques. pp. 10–12. U. S. Dept. Health, Education, and Welfare, PHS, NIH, Bethesda, 1968.
16.
TognellaS.: ricerche non pubblicate.1974.
17.
TognellaS., MantovaniG., Del GiaccoG. S., GrifoniV.: Electrophoretic mobility of lymphocytes in Hodgkin's disease, pp. 307. Abstr. 1.st Meeting Europ. Div. Intern. Soc. Haemat., Milano, 1971.
18.
ZeillerK.: Separation of lymphocyte sub-populations by use of free-flow electrophoresis. A comprehensive survey of recent research.Behring Inst. Mitt., 52: 11–25, 1972.
