Following a single dose of 3-methylcholanthrene in rat liver the RNA polymerase activity of Mg++ and Mn++/(NH4)2SO4 - dependent forms is stimulated in isolated nuclei and particularly in the chromatin of extranucleolar fraction. The early enhancement of N-2-fluorenylacetamide microsomal hydroxylating activity does not parallel the shift of the monomer-polysome equilibrium to more aggregated forms. It is suggested that the rate of synthesis and the transport mechanism of RNA messengers into the cytoplasm can be selectively regulated by 3-methylcholanthrene and its metabolites.
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